IL-1ra/IL-1F3/IL1RN Antibody (OTI2B1)

Novus Biologicals | Catalog # NBP2-45408

Novus Biologicals

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI2B1
View all IL-1ra/IL-1F3 Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human IL1RN (NP_776215) produced in E.coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Description

Novus Biologicals Mouse IL-1ra/IL-1F3/IL1RN Antibody (OTI2B1) (NBP2-45408) is a monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for IL-1ra/IL-1F3/IL1RN Antibody (OTI2B1)

Western Blot: IL-1ra/IL-1F3/IL1RN Antibody (OTI2B1) [NBP2-45408]

Western Blot: IL-1ra/IL-1F3/IL1RN Antibody (OTI2B1) [NBP2-45408]

Western Blot: IL-1ra/IL-1F3/IL1RN Antibody (2B1) [NBP2-45408] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IL1RN.
Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (OTI2B1) [NBP2-45408]

Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (OTI2B1) [NBP2-45408]

Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (2B1) [NBP2-45408] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (OTI2B1) [NBP2-45408]

Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (OTI2B1) [NBP2-45408]

Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (2B1) [NBP2-45408] - Analysis of Human breast tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (OTI2B1) [NBP2-45408]

Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (OTI2B1) [NBP2-45408]

Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (2B1) [NBP2-45408] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (OTI2B1) [NBP2-45408]

Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (OTI2B1) [NBP2-45408]

Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (2B1) [NBP2-45408] - Analysis of Human colon tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (OTI2B1) [NBP2-45408]

Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (OTI2B1) [NBP2-45408]

Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (2B1) [NBP2-45408] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (OTI2B1) [NBP2-45408]

Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (OTI2B1) [NBP2-45408]

Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (2B1) [NBP2-45408] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (OTI2B1) [NBP2-45408]

Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (OTI2B1) [NBP2-45408]

Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (2B1) [NBP2-45408] - Analysis of Carcinoma of Human kidney tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (OTI2B1) [NBP2-45408]

Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (OTI2B1) [NBP2-45408]

Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (2B1) [NBP2-45408] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (OTI2B1) [NBP2-45408]

Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (OTI2B1) [NBP2-45408]

Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (2B1) [NBP2-45408] - Analysis of Carcinoma of Human liver tissue.(Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (OTI2B1) [NBP2-45408]

Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (OTI2B1) [NBP2-45408]

Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (2B1) [NBP2-45408] - Analysis of Human lung tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (OTI2B1) [NBP2-45408]

Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (OTI2B1) [NBP2-45408]

Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (2B1) [NBP2-45408] - Analysis of Carcinoma of Human lung tissue.(Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (OTI2B1) [NBP2-45408]

Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (OTI2B1) [NBP2-45408]

Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (2B1) [NBP2-45408] - Analysis of Human Ovary tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (OTI2B1) [NBP2-45408]

Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (OTI2B1) [NBP2-45408]

Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (2B1) [NBP2-45408] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (OTI2B1) [NBP2-45408]

Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (OTI2B1) [NBP2-45408]

Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (2B1) [NBP2-45408] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (OTI2B1) [NBP2-45408]

Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (OTI2B1) [NBP2-45408]

Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (2B1) [NBP2-45408] - Analysis of Human thyroid tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (OTI2B1) [NBP2-45408]

Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (OTI2B1) [NBP2-45408]

Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (2B1) [NBP2-45408] - Analysis of Carcinoma of Human thyroid tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (OTI2B1) [NBP2-45408]

Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (OTI2B1) [NBP2-45408]

Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (2B1) [NBP2-45408] - Analysis of Human endometrium tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (OTI2B1) [NBP2-45408]

Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (OTI2B1) [NBP2-45408]

Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (2B1) [NBP2-45408] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (OTI2B1) [NBP2-45408]

Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (OTI2B1) [NBP2-45408]

Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (2B1) [NBP2-45408] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (OTI2B1) [NBP2-45408]

Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (OTI2B1) [NBP2-45408]

Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (2B1) [NBP2-45408] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (OTI2B1) [NBP2-45408]

Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (OTI2B1) [NBP2-45408]

Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (2B1) [NBP2-45408] - Analysis of Human bladder tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (OTI2B1) [NBP2-45408]

Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (OTI2B1) [NBP2-45408]

Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (2B1) [NBP2-45408] - Analysis of Carcinoma of Human bladder tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (OTI2B1) [NBP2-45408]

Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (OTI2B1) [NBP2-45408]

Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (2B1) [NBP2-45408] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (OTI2B1) [NBP2-45408]

Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (OTI2B1) [NBP2-45408]

Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (2B1) [NBP2-45408] - Analysis of Human lymphoma tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)

Applications for IL-1ra/IL-1F3/IL1RN Antibody (OTI2B1)

Application
Recommended Usage

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: IL-1ra/IL-1F3

IL-1ra (IL-1 receptor antagonist) is a widely expressed acute phase protein that serves to dampen inflammation. IL-1ra binds to both IL-1 RI and IL-1 RII. It competitively inhibits IL-1 (alpha or beta) binding to IL-1 RI and does not trigger recruitment of the accessory molecule IL-1 R AcP or activation of IL-1 RI signaling. IL-1ra blocks the proinflammatory actions of IL-1 including Prostaglandin E2 and IL-6 release from endothelial cells, fever generation, thrombocytosis, the production of hepatic acute phase proteins, and neutrophil expansion and infiltration.

Long Name

Interleukin 1 Receptor Antagonist

Alternate Names

DIRA, ICIL-1ra, IL-1F3, IL-1ra3, IL-1RN, IL1ra, IL1RN, MVCD4

Gene Symbol

IL1RN

Additional IL-1ra/IL-1F3 Products

Product Documents for IL-1ra/IL-1F3/IL1RN Antibody (OTI2B1)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for IL-1ra/IL-1F3/IL1RN Antibody (OTI2B1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for IL-1ra/IL-1F3/IL1RN Antibody (OTI2B1)

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    • Q: Can I use this antibody (IL-1ra/IL-1F3/IL1RN Antibody NBP2-45408; mouse monoclonal) for immunofluorescence staining of paraffin-embedded tissue?
      A: Yes, this product is guaranteed 100% for IHC on paraffin sections and feel free to use it on the said application with any detection method of your choice. The dilution that we suggested (1:150) is based on our testing using HRP-DAB detection and for IF based secondaries, you can do some pilot testing with a couple of different dilutions such as 1:150 and 1:500 to see what dilution gives you the best outcome.
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Associated Pathways

IL-1 Family Signaling Pathways IL-1 Family Signaling Pathway Thumbnail