IL-1ra (IL-1 receptor antagonist) is a widely expressed acute phase protein that serves to dampen inflammation. IL-1ra binds to both IL-1 RI and IL-1 RII. It competitively inhibits IL-1 (alpha or beta) binding to IL-1 RI and does not trigger recruitment of the accessory molecule IL-1 R AcP or activation of IL-1 RI signaling. IL-1ra blocks the proinflammatory actions of IL-1 including Prostaglandin E2 and IL-6 release from endothelial cells, fever generation, thrombocytosis, the production of hepatic acute phase proteins, and neutrophil expansion and infiltration.
IL-1ra/IL-1F3/IL1RN Antibody (OTI2B1)
Novus Biologicals | Catalog # NBP2-45408
![Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (OTI2B1) [NBP2-45408]](https://resources.rndsystems.com/images/products/IL-1ra-IL-1F3-IL1RN-Antibody-2B1-Immunohistochemistry-NBP2-45408-img0024.jpg "Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (OTI2B1) [NBP2-45408]")
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # OTI2B1
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Product Specifications
Immunogen
Full length human recombinant protein of human IL1RN (NP_776215) produced in E.coli.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for IL-1ra/IL-1F3/IL1RN Antibody (OTI2B1)
In support of our commitment to scientific transparency, some data images have been temporarily removed from this page pending internal review. Contact us for more information.
Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (OTI2B1) [NBP2-45408]
Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (2B1) [NBP2-45408] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)![Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (OTI2B1) [NBP2-45408]](https://resources.rndsystems.com/images/products/IL-1ra-IL-1F3-IL1RN-Antibody-2B1-Immunohistochemistry-NBP2-45408-img0001.jpg "Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (OTI2B1) [NBP2-45408]")
Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (OTI2B1) [NBP2-45408]
Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (2B1) [NBP2-45408] - Analysis of Human breast tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)![Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (OTI2B1) [NBP2-45408]](https://resources.rndsystems.com/images/products/IL-1ra-IL-1F3-IL1RN-Antibody-2B1-Immunohistochemistry-NBP2-45408-img0002.jpg "Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (OTI2B1) [NBP2-45408]")
Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (OTI2B1) [NBP2-45408]
Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (2B1) [NBP2-45408] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)![Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (OTI2B1) [NBP2-45408]](https://resources.rndsystems.com/images/products/IL-1ra-IL-1F3-IL1RN-Antibody-2B1-Immunohistochemistry-NBP2-45408-img0003.jpg "Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (OTI2B1) [NBP2-45408]")
Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (OTI2B1) [NBP2-45408]
Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (2B1) [NBP2-45408] - Analysis of Human colon tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)![Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (OTI2B1) [NBP2-45408]](https://resources.rndsystems.com/images/products/IL-1ra-IL-1F3-IL1RN-Antibody-2B1-Immunohistochemistry-NBP2-45408-img0004.jpg "Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (OTI2B1) [NBP2-45408]")
Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (OTI2B1) [NBP2-45408]
Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (2B1) [NBP2-45408] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)![Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (OTI2B1) [NBP2-45408]](https://resources.rndsystems.com/images/products/IL-1ra-IL-1F3-IL1RN-Antibody-2B1-Immunohistochemistry-NBP2-45408-img0005.jpg "Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (OTI2B1) [NBP2-45408]")
Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (OTI2B1) [NBP2-45408]
Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (2B1) [NBP2-45408] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)![Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (OTI2B1) [NBP2-45408]](https://resources.rndsystems.com/images/products/IL-1ra-IL-1F3-IL1RN-Antibody-2B1-Immunohistochemistry-NBP2-45408-img0006.jpg "Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (OTI2B1) [NBP2-45408]")
Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (OTI2B1) [NBP2-45408]
Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (2B1) [NBP2-45408] - Analysis of Carcinoma of Human kidney tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)![Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (OTI2B1) [NBP2-45408]](https://resources.rndsystems.com/images/products/IL-1ra-IL-1F3-IL1RN-Antibody-2B1-Immunohistochemistry-NBP2-45408-img0007.jpg "Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (OTI2B1) [NBP2-45408]")
Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (OTI2B1) [NBP2-45408]
Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (2B1) [NBP2-45408] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)![Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (OTI2B1) [NBP2-45408]](https://resources.rndsystems.com/images/products/IL-1ra-IL-1F3-IL1RN-Antibody-2B1-Immunohistochemistry-NBP2-45408-img0008.jpg "Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (OTI2B1) [NBP2-45408]")
Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (OTI2B1) [NBP2-45408]
Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (2B1) [NBP2-45408] - Analysis of Carcinoma of Human liver tissue.(Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)![Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (OTI2B1) [NBP2-45408]](https://resources.rndsystems.com/images/products/IL-1ra-IL-1F3-IL1RN-Antibody-2B1-Immunohistochemistry-NBP2-45408-img0009.jpg "Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (OTI2B1) [NBP2-45408]")
Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (OTI2B1) [NBP2-45408]
Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (2B1) [NBP2-45408] - Analysis of Human lung tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)![Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (OTI2B1) [NBP2-45408]](https://resources.rndsystems.com/images/products/IL-1ra-IL-1F3-IL1RN-Antibody-2B1-Immunohistochemistry-NBP2-45408-img0010.jpg "Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (OTI2B1) [NBP2-45408]")
Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (OTI2B1) [NBP2-45408]
Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (2B1) [NBP2-45408] - Analysis of Carcinoma of Human lung tissue.(Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)![Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (OTI2B1) [NBP2-45408]](https://resources.rndsystems.com/images/products/IL-1ra-IL-1F3-IL1RN-Antibody-2B1-Immunohistochemistry-NBP2-45408-img0011.jpg "Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (OTI2B1) [NBP2-45408]")
Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (OTI2B1) [NBP2-45408]
Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (2B1) [NBP2-45408] - Analysis of Human Ovary tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)![Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (OTI2B1) [NBP2-45408]](https://resources.rndsystems.com/images/products/IL-1ra-IL-1F3-IL1RN-Antibody-2B1-Immunohistochemistry-NBP2-45408-img0012.jpg "Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (OTI2B1) [NBP2-45408]")
Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (OTI2B1) [NBP2-45408]
Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (2B1) [NBP2-45408] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)![Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (OTI2B1) [NBP2-45408]](https://resources.rndsystems.com/images/products/IL-1ra-IL-1F3-IL1RN-Antibody-2B1-Immunohistochemistry-NBP2-45408-img0013.jpg "Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (OTI2B1) [NBP2-45408]")
Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (OTI2B1) [NBP2-45408]
Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (2B1) [NBP2-45408] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)![Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (OTI2B1) [NBP2-45408]](https://resources.rndsystems.com/images/products/IL-1ra-IL-1F3-IL1RN-Antibody-2B1-Immunohistochemistry-NBP2-45408-img0014.jpg "Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (OTI2B1) [NBP2-45408]")
Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (OTI2B1) [NBP2-45408]
Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (2B1) [NBP2-45408] - Analysis of Human thyroid tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)![Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (OTI2B1) [NBP2-45408]](https://resources.rndsystems.com/images/products/IL-1ra-IL-1F3-IL1RN-Antibody-2B1-Immunohistochemistry-NBP2-45408-img0015.jpg "Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (OTI2B1) [NBP2-45408]")
Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (OTI2B1) [NBP2-45408]
Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (2B1) [NBP2-45408] - Analysis of Carcinoma of Human thyroid tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)![Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (OTI2B1) [NBP2-45408]](https://resources.rndsystems.com/images/products/IL-1ra-IL-1F3-IL1RN-Antibody-2B1-Immunohistochemistry-NBP2-45408-img0016.jpg "Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (OTI2B1) [NBP2-45408]")
Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (OTI2B1) [NBP2-45408]
Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (2B1) [NBP2-45408] - Analysis of Human endometrium tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)![Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (OTI2B1) [NBP2-45408]](https://resources.rndsystems.com/images/products/IL-1ra-IL-1F3-IL1RN-Antibody-2B1-Immunohistochemistry-NBP2-45408-img0017.jpg "Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (OTI2B1) [NBP2-45408]")
Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (OTI2B1) [NBP2-45408]
Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (2B1) [NBP2-45408] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)![Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (OTI2B1) [NBP2-45408]](https://resources.rndsystems.com/images/products/IL-1ra-IL-1F3-IL1RN-Antibody-2B1-Immunohistochemistry-NBP2-45408-img0018.jpg "Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (OTI2B1) [NBP2-45408]")
Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (OTI2B1) [NBP2-45408]
Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (2B1) [NBP2-45408] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)![Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (OTI2B1) [NBP2-45408]](https://resources.rndsystems.com/images/products/IL-1ra-IL-1F3-IL1RN-Antibody-2B1-Immunohistochemistry-NBP2-45408-img0019.jpg "Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (OTI2B1) [NBP2-45408]")
Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (OTI2B1) [NBP2-45408]
Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (2B1) [NBP2-45408] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)![Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (OTI2B1) [NBP2-45408]](https://resources.rndsystems.com/images/products/IL-1ra-IL-1F3-IL1RN-Antibody-2B1-Immunohistochemistry-NBP2-45408-img0020.jpg "Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (OTI2B1) [NBP2-45408]")
Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (OTI2B1) [NBP2-45408]
Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (2B1) [NBP2-45408] - Analysis of Human bladder tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)![Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (OTI2B1) [NBP2-45408]](https://resources.rndsystems.com/images/products/IL-1ra-IL-1F3-IL1RN-Antibody-2B1-Immunohistochemistry-NBP2-45408-img0021.jpg "Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (OTI2B1) [NBP2-45408]")
Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (OTI2B1) [NBP2-45408]
Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (2B1) [NBP2-45408] - Analysis of Carcinoma of Human bladder tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)![Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (OTI2B1) [NBP2-45408]](https://resources.rndsystems.com/images/products/IL-1ra-IL-1F3-IL1RN-Antibody-2B1-Immunohistochemistry-NBP2-45408-img0022.jpg "Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (OTI2B1) [NBP2-45408]")
Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (OTI2B1) [NBP2-45408]
Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (2B1) [NBP2-45408] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)![Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (OTI2B1) [NBP2-45408]](https://resources.rndsystems.com/images/products/IL-1ra-IL-1F3-IL1RN-Antibody-2B1-Immunohistochemistry-NBP2-45408-img0023.jpg "Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (OTI2B1) [NBP2-45408]")
Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (OTI2B1) [NBP2-45408]
Immunohistochemistry: IL-1ra/IL-1F3/IL1RN Antibody (2B1) [NBP2-45408] - Analysis of Human lymphoma tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)Applications for IL-1ra/IL-1F3/IL1RN Antibody (OTI2B1)
Application
Recommended Usage
Immunohistochemistry
1:150
Immunohistochemistry-Paraffin
1:150
Western Blot
1:2000
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: IL-1ra/IL-1F3
Long Name
Interleukin 1 Receptor Antagonist
Alternate Names
DIRA, ICIL-1ra, IL-1F3, IL-1ra3, IL-1RN, IL1ra, IL1RN, MVCD4
Gene Symbol
IL1RN
Additional IL-1ra/IL-1F3 Products
Product Documents for IL-1ra/IL-1F3/IL1RN Antibody (OTI2B1)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for IL-1ra/IL-1F3/IL1RN Antibody (OTI2B1)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for IL-1ra/IL-1F3/IL1RN Antibody (OTI2B1)
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Q: Can I use this antibody (IL-1ra/IL-1F3/IL1RN Antibody NBP2-45408; mouse monoclonal) for immunofluorescence staining of paraffin-embedded tissue?
A: Yes, this product is guaranteed 100% for IHC on paraffin sections and feel free to use it on the said application with any detection method of your choice. The dilution that we suggested (1:150) is based on our testing using HRP-DAB detection and for IF based secondaries, you can do some pilot testing with a couple of different dilutions such as 1:150 and 1:500 to see what dilution gives you the best outcome.
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Associated Pathways
