Key Product Details
Species Reactivity
Canine
Applications
ELISA Standard (Matched Pair), ELISA Capture (Matched Antibody Pair), ELISA Detection (Matched Antibody Pair), Neutralization
Label
PerCP (Excitation = 490 nm, Emission = 675 nm)
Antibody Source
Polyclonal Goat IgG
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Product Specifications
Immunogen
E. coli-derived recombinant canine IL‑2 (R&D Systems, Catalog # 1815-CL)
Ala21-Thr155 with a Cys147Ser substitution
Accession # Q29416
Ala21-Thr155 with a Cys147Ser substitution
Accession # Q29416
Specificity
Detects canine IL-2 in ELISAs and Western blots. In sandwich immunoassays, less than 0.2% cross-reactivity with recombinant human IL‑2, recombinant mouse IL-2, recombinant rat IL-2, recombinant feline IL-2, recombinant cotton rat IL-2, recombinant bovine IL‑2, recombinant equine IL‑2, and recombinant porcine IL-2 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for IL-2 Antibody [PerCP]
Application
Recommended Usage
ELISA Capture (Matched Antibody Pair)
Optimal dilutions of this antibody should be experimentally determined.
ELISA Detection (Matched Antibody Pair)
Optimal dilutions of this antibody should be experimentally determined.
ELISA Standard (Matched Pair)
Optimal dilutions of this antibody should be experimentally determined.
Neutralization
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: IL-2
Long Name
Interleukin 2
Alternate Names
Aldesleukin, IL2, Proleukin, TCGF
Gene Symbol
IL2
Additional IL-2 Products
Product Documents for IL-2 Antibody [PerCP]
Product Specific Notices for IL-2 Antibody [PerCP]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- Troubleshooting Guide: ELISA
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
Innate Lymphoid Cell Differentiation Pathways
Jak/STAT Signaling Pathway
Th1 Differentiation Pathway
Th2 Differentiation Pathway