IL-20R alpha Antibody (MM0394-9F40) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-11695

Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # MM0394-9F40

Format

Azide and BSA Free
View all IL-20R alpha Primary Antibodies »

Product Specifications

Immunogen

Human recombinant IL-20R alpha (extracellular domain)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for IL-20R alpha Antibody (MM0394-9F40) - Azide and BSA Free

Immunohistochemistry-Paraffin: IL-20R alpha Antibody (MM0394-9F40) - Azide and BSA Free [NBP2-11695]

Immunohistochemistry-Paraffin: IL-20R alpha Antibody (MM0394-9F40) - Azide and BSA Free [NBP2-11695]

Immunohistochemistry-Paraffin: IL-20 R alpha Antibody (MM0394-9F40) [NBP2-11695] - 10% Buffer formalin fixed and parffine embedded human placetal tissue section (4um) is subjected IHC staining using NBP2-11695. Tissue section was pretreated in citric buffer (pH 6.0) with microwave for antige retrieval before IHC is applied.
IL-20R alpha Antibody (MM0394-9F40) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: IL-20R alpha Antibody (MM0394-9F40) - Azide and BSA Free [NBP2-11695] -

PDAC cell lines. (A) Laser scan microscopy and Western blotting of the PDAC cell lines AsPC-1, BxPC-3, and Panc-1 incubated with anti-IL10RB and anti-IL20RA antibodies shows expression and colocalization of both receptors in all three cell lines. (B) Western blotting of PDAC cells shows IL26 induced phosphorylation of STAT3 (left panel) and ERK1/2 (right panel) in a time-dependent manner. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/31948053), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for IL-20R alpha Antibody (MM0394-9F40) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:20-100

Immunohistochemistry-Paraffin

1:20-1:100

Western Blot

1:500-1:1000
Application Notes
Use in Immunocytochemistry/Immunofluorescence reported in scientific literature (PMID:31948053).

Formulation, Preparation, and Storage

Purification

Protein G purified

Reconstitution

Reconstitute with sterilized PBS to a final concentration of 0.5 mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS. 0.025 mg size is provided in liquid form, PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-20R alpha

Official Gene Symbol: IL20RA Gene ID: 53832 (Human) Gene Map Locus: 6q22.33-q23.1 (Human) IL20RA, is a subunit of IL20R, a member of Cytokine Class II receptor super family. It consists of a central transmembrane domain and functionally induces IL20 and IL26 signaling in epithelial cells and keratinocytes. It associates with IL-20RB and forms a functional receptor complex for IL20, IL19, and IL24. It also associates with IL-10RB and forms a unique heterodimeric functional receptor complex for IL-26. Upon ligand binding, it induces signaling through activation of STAT3 and STAT1. IL20RA is specifically expressed in skin suggesting a prominent role in growth and proliferation of keratinocytes.

Long Name

Interleukin 20 Receptor alpha

Alternate Names

CRF2-8, IL-20 R alpha, IL-20R1, IL-20Ra, IL20R alpha, IL20RA, zcytor7

Gene Symbol

IL20RA

UniProt

Q9UHF4

Additional IL-20R alpha Products

Product Documents for IL-20R alpha Antibody (MM0394-9F40) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for IL-20R alpha Antibody (MM0394-9F40) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for IL-20R alpha Antibody (MM0394-9F40) - Azide and BSA Free

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Protocols

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