IL-22R alpha 1 Antibody - BSA Free
Novus Biologicals | Catalog # NBP1-76724
![Western Blot: IL-22R alpha 1 AntibodyBSA Free [NBP1-76724]](https://resources.rndsystems.com/images/products/IL-22R-alpha-1-Antibody-Western-Blot-NBP1-76724-img0010.jpg "Western Blot: IL-22R alpha 1 AntibodyBSA Free [NBP1-76724]")
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Mouse
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence
Cited:
Flow Cytometry
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
Antibody was raised against a synthetic peptide corresponding to amino acids near the amino terminus of human IL-22 receptor precursor. The immunogen is located within the first 50 amino acids of IL-22 Receptor.
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Bovine (88%). Use in Mouse reported in scientific literature (PMID: 32127533). Use in Mouse reported in scientific literature (PMID:32127533).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
65 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for IL-22R alpha 1 Antibody - BSA Free
Western Blot: IL-22R alpha 1 AntibodyBSA Free [NBP1-76724]
Western Blot: IL-22R alpha 1 Antibody [NBP1-76724] - Analysis of IL-22 receptor expression in human HepG2 cell lysate with IL-22 receptor antibody at (A) 0.5 and (B) 1 ug /ml.![Immunohistochemistry: IL-22R alpha 1 Antibody - BSA Free [NBP1-76724]](https://resources.rndsystems.com/images/products/IL-22R-alpha-1-Antibody-Immunohistochemistry-NBP1-76724-img0007.jpg "Immunohistochemistry: IL-22R alpha 1 Antibody - BSA Free [NBP1-76724]")
Immunohistochemistry: IL-22R alpha 1 Antibody - BSA Free [NBP1-76724]
Immunohistochemistry: IL-22R alpha 1 Antibody [NBP1-76724] - Staining of rat kidney tissue with antibody at 5 ug/mL.![Immunocytochemistry/ Immunofluorescence: IL-22R alpha 1 Antibody - BSA Free [NBP1-76724]](https://resources.rndsystems.com/images/products/IL-22R-alpha-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-76724-img0008.jpg "Immunocytochemistry/ Immunofluorescence: IL-22R alpha 1 Antibody - BSA Free [NBP1-76724]")
Immunocytochemistry/ Immunofluorescence: IL-22R alpha 1 Antibody - BSA Free [NBP1-76724]
Immunocytochemistry/Immunofluorescence: IL-22R alpha 1 Antibody [NBP1-76724] - Immunofluorescence:HepG2 cells with IL-22RA1 receptor antibody at 10 ug/ml.
Immunocytochemistry/ Immunofluorescence: IL-22R alpha 1 Antibody - BSA Free [NBP1-76724] -
Immunocytochemistry/ Immunofluorescence: IL-22R alpha 1 Antibody - BSA Free [NBP1-76724] - Immunofluorescence of IL-22R alpha 1 in rat kidney tissue with IL-22R alpha 1 antibody at 20 u/ml.
Immunocytochemistry/ Immunofluorescence: IL-22R alpha 1 Antibody - BSA Free [NBP1-76724] -
Immunocytochemistry/ Immunofluorescence: IL-22R alpha 1 Antibody - BSA Free [NBP1-76724] - Immunofluorescence of IL-22 receptor in HepG2 cells with IL-22 receptor antibody at 10 ug/mL.Applications for IL-22R alpha 1 Antibody - BSA Free
Application
Recommended Usage
ELISA
1:100-1:2000
Immunocytochemistry/ Immunofluorescence
10 ug/ml
Immunohistochemistry
5 ug/mL
Immunohistochemistry-Paraffin
5 ug/mL
Western Blot
0.5-1 ug/ml
Application Notes
Use in Flow Cytometry reported in scientific literature (PMID:32127533).
Flow Cytometry Panel Builder
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Peptide affinity purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: IL-22 R alpha 1
Long Name
Interleukin 22 Receptor
Alternate Names
CRF2-9, IL-22Ra1, IL-TIF-R1, IL22R alpha 1, IL22RA1
Gene Symbol
IL22RA1
UniProt
Additional IL-22 R alpha 1 Products
Product Documents for IL-22R alpha 1 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for IL-22R alpha 1 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Citations for IL-22R alpha 1 Antibody - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Liperfluo
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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