![Western Blot: IL-3 Antibody (OTI3B11) [NBP1-47806]](https://resources.rndsystems.com/images/products/IL-3-Antibody-3B11-Western-Blot-NBP1-47806-img0022.jpg "Western Blot: IL-3 Antibody (OTI3B11) [NBP1-47806]")
Discontinued Product
NBP1-47806 has been discontinued.
View all IL-3 products.
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # OTI3B11
Loading...
Product Specifications
Immunogen
Human recombinant protein fragment corresponding to amino acids 20-152 of human IL-3 (NP_000579) produced in E.coli.
Specificity
This antibody is specific for Homo sapiens interleukin 3 (colony-stimulating factor, multiple) (IL3).
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
15 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for IL-3 Antibody (OTI3B11)
Western Blot: IL-3 Antibody (OTI3B11) [NBP1-47806]
Western Blot: IL-3 Antibody (3B11) [NBP1-47806] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IL3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IL3.![Immunocytochemistry/ Immunofluorescence: IL-3 Antibody (OTI3B11) [NBP1-47806]](https://resources.rndsystems.com/images/products/IL-3-Antibody-3B11-Immunocytochemistry-Immunofluorescence-NBP1-47806-img0013.jpg "Immunocytochemistry/ Immunofluorescence: IL-3 Antibody (OTI3B11) [NBP1-47806]")
Immunocytochemistry/ Immunofluorescence: IL-3 Antibody (OTI3B11) [NBP1-47806]
Immunocytochemistry/Immunofluorescence: IL-3 Antibody (3B11) [NBP1-47806] - Staining of A549 cells using anti-IL3 mouse monoclonal antibody.![Immunohistochemistry-Paraffin: IL-3 Antibody (OTI3B11) [NBP1-47806]](https://resources.rndsystems.com/images/products/IL-3-Antibody-3B11-Immunohistochemistry-Paraffin-NBP1-47806-img0024.jpg "Immunohistochemistry-Paraffin: IL-3 Antibody (OTI3B11) [NBP1-47806]")
Immunohistochemistry-Paraffin: IL-3 Antibody (OTI3B11) [NBP1-47806]
Immunohistochemistry-Paraffin: IL-3 Antibody (3B11) [NBP1-47806] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue.![Immunohistochemistry-Paraffin: IL-3 Antibody (OTI3B11) [NBP1-47806]](https://resources.rndsystems.com/images/products/IL-3-Antibody-3B11-Immunohistochemistry-Paraffin-NBP1-47806-img0014.jpg "Immunohistochemistry-Paraffin: IL-3 Antibody (OTI3B11) [NBP1-47806]")
Immunohistochemistry-Paraffin: IL-3 Antibody (OTI3B11) [NBP1-47806]
Immunohistochemistry-Paraffin: IL-3 Antibody (3B11) [NBP1-47806] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-IL3 mouse monoclonal antibody.![Immunohistochemistry-Paraffin: IL-3 Antibody (OTI3B11) [NBP1-47806]](https://resources.rndsystems.com/images/products/IL-3-Antibody-3B11-Immunohistochemistry-Paraffin-NBP1-47806-img0015.jpg "Immunohistochemistry-Paraffin: IL-3 Antibody (OTI3B11) [NBP1-47806]")
Immunohistochemistry-Paraffin: IL-3 Antibody (OTI3B11) [NBP1-47806]
Immunohistochemistry-Paraffin: IL-3 Antibody (3B11) [NBP1-47806] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-IL3 mouse monoclonal antibody.![Immunohistochemistry-Paraffin: IL-3 Antibody (OTI3B11) [NBP1-47806]](https://resources.rndsystems.com/images/products/IL-3-Antibody-3B11-Immunohistochemistry-Paraffin-NBP1-47806-img0016.jpg "Immunohistochemistry-Paraffin: IL-3 Antibody (OTI3B11) [NBP1-47806]")
Immunohistochemistry-Paraffin: IL-3 Antibody (OTI3B11) [NBP1-47806]
Immunohistochemistry-Paraffin: IL-3 Antibody (3B11) [NBP1-47806] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-IL3 mouse monoclonal antibody.![Immunohistochemistry-Paraffin: IL-3 Antibody (OTI3B11) [NBP1-47806]](https://resources.rndsystems.com/images/products/IL-3-Antibody-3B11-Immunohistochemistry-Paraffin-NBP1-47806-img0017.jpg "Immunohistochemistry-Paraffin: IL-3 Antibody (OTI3B11) [NBP1-47806]")
Immunohistochemistry-Paraffin: IL-3 Antibody (OTI3B11) [NBP1-47806]
Immunohistochemistry-Paraffin: IL-3 Antibody (3B11) [NBP1-47806] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-IL3 mouse monoclonal antibody.![Immunohistochemistry-Paraffin: IL-3 Antibody (OTI3B11) [NBP1-47806]](https://resources.rndsystems.com/images/products/IL-3-Antibody-3B11-Immunohistochemistry-Paraffin-NBP1-47806-img0018.jpg "Immunohistochemistry-Paraffin: IL-3 Antibody (OTI3B11) [NBP1-47806]")
Immunohistochemistry-Paraffin: IL-3 Antibody (OTI3B11) [NBP1-47806]
Immunohistochemistry-Paraffin: IL-3 Antibody (3B11) [NBP1-47806] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-IL3 mouse monoclonal antibody.![Immunohistochemistry-Paraffin: IL-3 Antibody (OTI3B11) [NBP1-47806]](https://resources.rndsystems.com/images/products/IL-3-Antibody-3B11-Immunohistochemistry-Paraffin-NBP1-47806-img0019.jpg "Immunohistochemistry-Paraffin: IL-3 Antibody (OTI3B11) [NBP1-47806]")
Immunohistochemistry-Paraffin: IL-3 Antibody (OTI3B11) [NBP1-47806]
Immunohistochemistry-Paraffin: IL-3 Antibody (3B11) [NBP1-47806] - Staining of paraffin-embedded Human breast tissue using anti-IL3 mouse monoclonal antibody.![Immunohistochemistry-Paraffin: IL-3 Antibody (OTI3B11) [NBP1-47806]](https://resources.rndsystems.com/images/products/IL-3-Antibody-3B11-Immunohistochemistry-Paraffin-NBP1-47806-img0020.jpg "Immunohistochemistry-Paraffin: IL-3 Antibody (OTI3B11) [NBP1-47806]")
Immunohistochemistry-Paraffin: IL-3 Antibody (OTI3B11) [NBP1-47806]
Immunohistochemistry-Paraffin: IL-3 Antibody (3B11) [NBP1-47806] - Staining of paraffin-embedded Human colon tissue using anti-IL3 mouse monoclonal antibody.![Immunohistochemistry-Paraffin: IL-3 Antibody (OTI3B11) [NBP1-47806]](https://resources.rndsystems.com/images/products/IL-3-Antibody-3B11-Immunohistochemistry-Paraffin-NBP1-47806-img0021.jpg "Immunohistochemistry-Paraffin: IL-3 Antibody (OTI3B11) [NBP1-47806]")
Immunohistochemistry-Paraffin: IL-3 Antibody (OTI3B11) [NBP1-47806]
Immunohistochemistry-Paraffin: IL-3 Antibody (3B11) [NBP1-47806] - Staining of paraffin-embedded Human Kidney tissue using anti-IL3 mouse monoclonal antibody.![Immunohistochemistry-Paraffin: IL-3 Antibody (OTI3B11) [NBP1-47806]](https://resources.rndsystems.com/images/products/IL-3-Antibody-3B11-Immunohistochemistry-Paraffin-NBP1-47806-img0023.jpg "Immunohistochemistry-Paraffin: IL-3 Antibody (OTI3B11) [NBP1-47806]")
Immunohistochemistry-Paraffin: IL-3 Antibody (OTI3B11) [NBP1-47806]
Immunohistochemistry-Paraffin: IL-3 Antibody (3B11) [NBP1-47806] - Staining of paraffin-embedded Human breast tissue.Applications for IL-3 Antibody (OTI3B11)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100
Immunohistochemistry
1:50
Immunohistochemistry-Paraffin
1:50
Western Blot
1:500
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: IL-3
Long Name
Interleukin 3
Alternate Names
IL3, MCGF, MULTI-CSF
Entrez Gene IDs
3562 (Human)
Gene Symbol
IL3
Additional IL-3 Products
Product Documents for IL-3 Antibody (OTI3B11)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for IL-3 Antibody (OTI3B11)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Customer Reviews for IL-3 Antibody (OTI3B11)
There are currently no reviews for this product. Be the first to review IL-3 Antibody (OTI3B11) and earn rewards!
Have you used IL-3 Antibody (OTI3B11)?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 Yen for a review with an image
$10/€7/£6/$10CAN/¥1110 Yen for a review without an image
Submit a review
Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
