Rat IL-5 ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # NBP3-06769

Novus Biologicals
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma, cell supernatant, and other biological fluids (100 ul)

Sensitivity

9.38 pg/mL

Assay Range

15.63 - 1000 pg/mL
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Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Rat

Specificity

This kit recognizes Rat IL-5 in samples. No significant cross-reactivity or interference between Rat IL-5 and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) 5.27

Inter-Assay Precision (Precision between assays) 5.15

Recovery for Rat IL-5 ELISA Kit (Colorimetric)

Recovery

85 - 107 %

Linearity

Rat IL-5 ELISA Kit (Colorimetric)

Scientific Data Images for Rat IL-5 ELISA Kit (Colorimetric)

Rat IL-5 ELISA Kit (Colorimetric)

ELISA: Rat IL-5 ELISA Kit (Colorimetric) [NBP3-06769] -

ELISA: Rat IL-5 ELISA Kit (Colorimetric) [NBP3-06769] - Standard Curve Reference

Kit Contents for Rat IL-5 ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: IL-5

IL-5 (T cell replacing factor (TRF), eosinophil differentiation factor (EDF)) is a hematopoietic growth factor that stimulates the proliferation, differentiation and activation of eosinophils. It is produced by mast cells, T cells and eosinophils and has an important role in activating cell-mediated immunity following parasitic infections. It belongs to the same cytokine family as IL-3 and GM-CSF. The biologically active IL-5 consists of an antiparallel homodimer linked by disulfide bonds. Human and murine IL-5 are 70% identical at the amino acid level and show species cross-reactivity. IL-5 exerts its effects on target cells by binding to specific cell surface receptors.

Long Name

Interleukin 5

Alternate Names

BCDF mu, BCGFII, EDF, Eo-CSF, IL5, TRF

Gene Symbol

IL5

Additional IL-5 Products

Product Documents for Rat IL-5 ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Rat IL-5 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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  • Rat IL-5 ELISA Kit (Colorimetric)
    Name: Anonymous
    Sample Tested: Serum and Heparin Plasma
    Verified Customer | Posted 06/13/2022
    Interleukin 5 - main experiment.
    ZDF rats, diabetic model. Influence of vitamin K2 on cytokine expression with regard to disease pathogenesis. Dilutions between 1:3 and 1:5 proved optimal.
    Rat IL-5 ELISA Kit (Colorimetric) NBP3-06769

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