ILF1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-87700

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (90%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Chromatin Immunoprecipitation-exo-Seq

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all ILF1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: SSQPVLITVQRQLPQAIKPVTYTVATPVTTSTSQPPVVQTVHVVHQIPAVSVTSVAGLAPANTYTVSGQAVVTPAAVLAPPKAEAQENGDHREVKVKVEPIPAIGHATLGTASRIIQTAQTTPVQTVTIVQQAPLGQHQLPIK

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ILF1 Antibody - BSA Free

Western Blot: ILF1 Antibody [NBP1-87700]

Western Blot: ILF1 Antibody [NBP1-87700]

Western Blot: ILF1 Antibody [NBP1-87700] - Whole cell lysates from SUM159, MDA-MB-231, MCF-7 and HCC1954 cells were loaded with 50 ug/lane. 10% SDS-PAGE. ILF1 antibody (NBP1-87700) was used for primary antibody: 1:2000, 4C, overnight. Image from verified customer review.
Immunocytochemistry/ Immunofluorescence: ILF1 Antibody [NBP1-87700]

Immunocytochemistry/ Immunofluorescence: ILF1 Antibody [NBP1-87700]

Immunocytochemistry/Immunofluorescence: ILF1 Antibody [NBP1-87700] - Staining of human cell line U-2 OS shows localization to nucleoplasm & vesicles. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: ILF1 Antibody [NBP1-87700]

Immunohistochemistry-Paraffin: ILF1 Antibody [NBP1-87700]

Immunohistochemistry-Paraffin: ILF1 Antibody [NBP1-87700] - Staining of human small intestine shows distinct nuclear positivity in glandular cells.
Western Blot: ILF1 Antibody [NBP1-87700]

Western Blot: ILF1 Antibody [NBP1-87700]

Western Blot: ILF1 Antibody [NBP1-87700] - Analysis in human cell line RT-4 and human cell line U-251 MG.
ILF1 Antibody - BSA Free Chromatin Immunoprecipitation-exo-Seq: ILF1 Antibody - BSA Free [NBP1-87700]

Chromatin Immunoprecipitation-exo-Seq: ILF1 Antibody - BSA Free [NBP1-87700]

ChIP-Exo-Seq composite graph for Anti-FOXK2 (NBP1-87700) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.
ILF1 Antibody - BSA Free

Western Blot: ILF1 Antibody - BSA Free [NBP1-87700] -

Foxk1 and Foxk2 extend the cardiomyocyte proliferative window.A Experimental design: neonatal mice were administered AAV9-GFP, AAV9-Foxk1, or AAV9-Foxk2 on postnatal day 1 (P1) to overexpress Foxk1 or Foxk2 specifically in cardiomyocytes. Heart tissue was subsequently collected at P14. B Western blot analysis of FOXK1 and FOXK2 expression in P14 mouse cardiomyocytes (n = 3 mice per group). C H&E-stained heart sections (n = 5 mice per group). D, E WGA staining of heart tissue sections and quantitative analysis of the cross-sectional area of cardiomyocytes (CM size) (n = 3 per group). F Analysis of total cardiomyocyte counts per heart across different experimental groups (n = 6 per group). G Representative images of cardiomyocytes isolated from the hearts of mice treated with AAV9-Foxk1 or AAV9-Foxk2 and quantification of nucleation at P14 (n = 3 per group). H–J Representative images of co-immunostaining and quantitative analysis for alpha -actinin (red) and Aurora B (green, indicated by yellow arrows) (H), pH3 (green) (I), and Ki67 (green) (J) in heart tissue sections (n = 4 per group). Data are presented as mean +/- SEM. p values were determined by one-way ANOVA with Bonferroni multiple comparisons test (E, F, and H–J) and two-way ANOVA with Bonferroni multiple comparisons test (G). Source data are provided as a Source Data file. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/40128196), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for ILF1 Antibody - BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation-exo-Seq

1-10ug per reaction

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Reviewed Applications

Read 1 review rated 5 using NBP1-87700 in the following applications:

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ILF1

ILF1 is encoded by this gene contains a fork head DNA binding domain. This protein can bind to the purine-rich motifs of the HIV long terminal repeat (LTR), and to the similar purine-rich motif in the interleukin 2 (IL2) promoter. It may be involved in the regulation of viral and cellular promoter elements. [provided by RefSeq]

Alternate Names

Cellular transcription factor ILF-1, forkhead box K2, forkhead box protein K2, FOXK1, ILF-1, ILF1ILF, interleukin enhancer binding factor 1, Interleukin enhancer-binding factor 1

Gene Symbol

FOXK2

Additional ILF1 Products

Product Documents for ILF1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for ILF1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for ILF1 Antibody - BSA Free

Customer Reviews for ILF1 Antibody - BSA Free (1)

5 out of 5
1 Customer Rating
5 Stars
100%
4 Stars
0%
3 Stars
0%
2 Stars
0%
1 Stars
0%

Have you used ILF1 Antibody - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Customer Images

Showing  1 - 1 of 1 review Showing All
Filter By:
Clear Filters X
  • ILF1 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: SUM-159PT, MDA-MB-231 and MCF-7
    Species: Human
    Verified Customer | Posted 11/15/2022
    Western Blot: whole cell lysates from SUM159, MDA-MB-231, MCF-7 and HCC1954 cells were loaded with 50 ug/lane. 10% SDS-PAGE. ILF1 Antibody (NBP1-87700) was used for primary antibody: 1:2000, 4℃, overnight.
    ILF1 Antibody - BSA Free NBP1-87700

There are no reviews that match your criteria.

Showing  1 - 1 of 1 review Showing All

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies