ILF3 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-84056

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (92%), Rat (92%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all ILF3 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: VMAKHSSVYPTQEELEAVQNMVSHTERALKAVSDWIDEQEKGSSEQAESDNMDVPPEDDSKEGAGEQKTEHMTRTLRGVMRVGLVAKGLLLKGDLDLELVLLCKEKPTTALLDKVADNLAIQLAAVTEDKYEILQSVDDAAIVIK

Reactivity Notes

Reactivity reported in scientific literature (PMID: 24098121)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ILF3 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: ILF3 Antibody [NBP1-84056]

Immunocytochemistry/ Immunofluorescence: ILF3 Antibody [NBP1-84056]

Immunocytochemistry/Immunofluorescence: ILF3 Antibody [NBP1-84056] - Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm, nucleoli & mitochondria. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: ILF3 Antibody [NBP1-84056]

Immunohistochemistry-Paraffin: ILF3 Antibody [NBP1-84056]

Immunohistochemistry-Paraffin: ILF3 Antibody [NBP1-84056] - Staining of human pancreas shows strong nuclear positivity in exocrine glandular cells.
Immunohistochemistry-Paraffin: ILF3 Antibody [NBP1-84056]

Immunohistochemistry-Paraffin: ILF3 Antibody [NBP1-84056]

Immunohistochemistry-Paraffin: ILF3 Antibody [NBP1-84056] - Staining of human cerebral cortex shows strong nuclear positivity in neurons.
Immunohistochemistry-Paraffin: ILF3 Antibody [NBP1-84056]

Immunohistochemistry-Paraffin: ILF3 Antibody [NBP1-84056]

Immunohistochemistry-Paraffin: ILF3 Antibody [NBP1-84056] - Staining of human lymph node shows strong nuclear positivity in germinal center cells.
Immunohistochemistry-Paraffin: ILF3 Antibody [NBP1-84056]

Immunohistochemistry-Paraffin: ILF3 Antibody [NBP1-84056]

Immunohistochemistry-Paraffin: ILF3 Antibody [NBP1-84056] - Staining of human lymph node shows strong nuclear positivity in cells in seminiferous ducts.
ILF3 Antibody - BSA Free Western Blot: ILF3 Antibody - BSA Free [NBP1-84056]

Western Blot: ILF3 Antibody - BSA Free [NBP1-84056]

Analysis in human cell line MOLT-4.
ILF3 Antibody - BSA Free Chromatin Immunoprecipitation-exo-Seq: ILF3 Antibody - BSA Free [NBP1-84056]

Chromatin Immunoprecipitation-exo-Seq: ILF3 Antibody - BSA Free [NBP1-84056]

ChIP-Exo-Seq composite graph for Anti-ILF3 (NBP1-84056) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.

Applications for ILF3 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:2500 - 1:5000

Immunohistochemistry-Paraffin

1:2500 - 1:5000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ILF3

ILF3 encodes a double-stranded RNA (dsRNA) binding protein that complexes with other proteins, dsRNAs, small noncoding RNAs, and mRNAs to regulate gene expression and stabilize mRNAs. This protein was first discovered to be a subunit of the nuclear factor of activated T-cells (NFAT); a transcription factor required for T-cell expression of interleukin 2. NFAT is a heterodimer of 45 kDa and 90 kDa proteins, the larger of which is the product of this gene. These proteins have been shown to affect the redistribution of nuclear mRNA to the cytoplasm. Knockdown of NF45 or NF90 protein retards cell growth; possibly by inhibition of mRNA stabilization. In contrast, an isoform (NF110) of this gene that is predominantly restricted to the nucleus has only minor effects on cell growth when its levels are reduced. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

Alternate Names

Double-stranded RNA-binding protein 76, double-stranded RNA-binding protein, 76 kD, DRBF, DRBP76M-phase phosphoprotein 4, dsRNA binding protein NFAR-2/MPP4, interleukin enhancer binding factor 3, 90kDa, interleukin enhancer-binding factor 3, MPHOSPH4interleukin enhancer binding factor 3, 90kD, MPP4MMP4, NF90CBTF, NFAR-1, NFAR2, NFARNF110, NF-AT-90, Nuclear factor associated with dsRNA, Nuclear factor of activated T-cells 90 kDa, nuclear factor of activated T-cells, 90 kD, TCP110, TCP80, Translational control protein 80

Gene Symbol

ILF3

Additional ILF3 Products

Product Documents for ILF3 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for ILF3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for ILF3 Antibody - BSA Free

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Protocols

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FAQs

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