Importin alpha 2/KPNA2 Antibody (1E8B7) - BSA Free

Novus Biologicals | Catalog # NBP2-52501

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 1E8B7

Format

BSA Free
View all Importin alpha 2/KPNA2 Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human Importin alpha 2/KPNA2 (AA: 1-530) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

58 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Importin alpha 2/KPNA2 Antibody (1E8B7) - BSA Free

Western Blot: Importin alpha 2/KPNA2 Antibody (1E8B7)BSA Free [NBP2-52501]

Western Blot: Importin alpha 2/KPNA2 Antibody (1E8B7)BSA Free [NBP2-52501]

Western Blot: Importin alpha 2/KPNA2 Antibody (1E8B7) [NBP2-52501] - Analysis using KPNA2 mAb against HEK293 (1) and KPNA2 (AA: 1-530)-hIgGFc transfected HEK293 (2) cell lysate.
Immunocytochemistry/ Immunofluorescence: Importin alpha 2/KPNA2 Antibody (1E8B7) - BSA Free [NBP2-52501]

Immunocytochemistry/ Immunofluorescence: Importin alpha 2/KPNA2 Antibody (1E8B7) - BSA Free [NBP2-52501]

Immunocytochemistry/Immunofluorescence: Importin alpha 2/KPNA2 Antibody (1E8B7) [NBP2-52501] - Analysis of Hela cells using KPNA2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
Immunohistochemistry-Paraffin: Importin alpha 2/KPNA2 Antibody (1E8B7) - BSA Free [NBP2-52501]

Immunohistochemistry-Paraffin: Importin alpha 2/KPNA2 Antibody (1E8B7) - BSA Free [NBP2-52501]

Immunohistochemistry-Paraffin: Importin alpha 2/KPNA2 Antibody (1E8B7) [NBP2-52501] - Analysis of paraffin-embedded esophageal cancer tissues using KPNA2 mouse mAb with DAB staining.
Flow Cytometry: Importin alpha 2/KPNA2 Antibody (1E8B7) - BSA Free [NBP2-52501]

Flow Cytometry: Importin alpha 2/KPNA2 Antibody (1E8B7) - BSA Free [NBP2-52501]

Flow Cytometry: Importin alpha 2/KPNA2 Antibody (1E8B7) [NBP2-52501] - Analysis of Hela cells using KPNA2 mouse mAb (green) and negative control (red).
Western Blot: Importin alpha 2/KPNA2 Antibody (1E8B7)BSA Free [NBP2-52501]

Western Blot: Importin alpha 2/KPNA2 Antibody (1E8B7)BSA Free [NBP2-52501]

Western Blot: Importin alpha 2/KPNA2 Antibody (1E8B7) [NBP2-52501] - Analysis using Importin alpha 2/KPNA2 against human KPNA2 (AA: 1-530) recombinant protein. (Expected MW is 60.3 kDa)
Western Blot: Importin alpha 2/KPNA2 Antibody (1E8B7)BSA Free [NBP2-52501]

Western Blot: Importin alpha 2/KPNA2 Antibody (1E8B7)BSA Free [NBP2-52501]

Western Blot: Importin alpha 2/KPNA2 Antibody (1E8B7) [NBP2-52501] - Analysis using Importin alpha 2/KPNA2 against HEK293 (1) and KPNA2 (AA: 1-530)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot: Importin alpha 2/KPNA2 Antibody (1E8B7)BSA Free [NBP2-52501]

Western Blot: Importin alpha 2/KPNA2 Antibody (1E8B7)BSA Free [NBP2-52501]

Western Blot: Importin alpha 2/KPNA2 Antibody (1E8B7) [NBP2-52501] - Analysis using KPNA2 mouse mAb against Hela (1), HEK293 (2), and NIH/3T3 (3) cell lysate.
Immunocytochemistry/ Immunofluorescence: Importin alpha 2/KPNA2 Antibody (1E8B7) - BSA Free [NBP2-52501]

Immunocytochemistry/ Immunofluorescence: Importin alpha 2/KPNA2 Antibody (1E8B7) - BSA Free [NBP2-52501]

Immunocytochemistry/Immunofluorescence: Importin alpha 2/KPNA2 Antibody (1E8B7) [NBP2-52501] - Analysis of Hela cells using KPNA2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
Immunohistochemistry-Paraffin: Importin alpha 2/KPNA2 Antibody (1E8B7) - BSA Free [NBP2-52501]

Immunohistochemistry-Paraffin: Importin alpha 2/KPNA2 Antibody (1E8B7) - BSA Free [NBP2-52501]

Immunohistochemistry-Paraffin: Importin alpha 2/KPNA2 Antibody (1E8B7) [NBP2-52501] - Analysis of paraffin-embedded lung cancer tissues using KPNA2 mouse mAb with DAB staining.
Flow Cytometry: Importin alpha 2/KPNA2 Antibody (1E8B7) - BSA Free [NBP2-52501]

Flow Cytometry: Importin alpha 2/KPNA2 Antibody (1E8B7) - BSA Free [NBP2-52501]

Flow Cytometry: Importin alpha 2/KPNA2 Antibody (1E8B7) [NBP2-52501] - Analysis of Hela cells using KPNA2 mouse mAb (green) and negative control (red).
ELISA: Importin alpha 2/KPNA2 Antibody (1E8B7) - BSA Free [NBP2-52501]

ELISA: Importin alpha 2/KPNA2 Antibody (1E8B7) - BSA Free [NBP2-52501]

ELISA: Importin alpha 2/KPNA2 Antibody (1E8B7) [NBP2-52501] - 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Applications for Importin alpha 2/KPNA2 Antibody (1E8B7) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200-1:400

Immunocytochemistry/ Immunofluorescence

1:200-1:1000

Immunohistochemistry

1:200-1:1000

Immunohistochemistry-Paraffin

1:200 - 1:1000

Western Blot

1:500-1:2000
Application Notes
This antibody is Cytof ready.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Importin alpha 2/KPNA2

The import of proteins into the nucleus is a process that involves at least 2 steps. The first is an energy-independent docking of the protein to the nuclear envelope and the second is an energy-dependent translocation through the nuclear pore complex. Imported proteins require a nuclear localization sequence (NLS) which generally consists of a short region of basic amino acids or 2 such regions spaced about 10 amino acids apart. Proteins involved in the first step of nuclear import have been identified in different systems. These include the Xenopus protein importin and its yeast homolog, SRP1 (a suppressor of certain temperature-sensitive mutations of RNA polymerase I in Saccharomyces cerevisiae), which bind to the NLS. KPNA2 protein interacts with the NLSs of DNA helicase Q1 and SV40 T antigen and may be involved in the nuclear transport of proteins. KPNA2 also may play a role in V(D)J recombination

Long Name

Karyopherin alpha 2

Alternate Names

Importin alpha 1, IPOA1, KPNA2, Pendulin, QIP2, RCH1, SRP1-alpha

Gene Symbol

KPNA2

Additional Importin alpha 2/KPNA2 Products

Product Documents for Importin alpha 2/KPNA2 Antibody (1E8B7) - BSA Free

Certificate of Analysis

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Product Specific Notices for Importin alpha 2/KPNA2 Antibody (1E8B7) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cancer Biomarkers
Cell Cycle

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Protocols

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FAQs

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