Indoleamine 2,3-dioxygenase/IDO Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-87702

Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Simple Western

Cited:

Immunohistochemistry-Paraffin, Western Blot, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Indoleamine 2,3-dioxygenase/IDO Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: LCSLESNPSVREFVLSKGDAGLREAYDACVKALVSLRSYHLQIVTKYILIPASQQPKENKTSEDPSKLEAKGTGGTDLMNFLK

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Indoleamine 2,3-dioxygenase/IDO Antibody - BSA Free

Immunohistochemistry-Paraffin: Indoleamine 2,3-dioxygenase/IDO Antibody [NBP1-87702]

Immunohistochemistry-Paraffin: Indoleamine 2,3-dioxygenase/IDO Antibody [NBP1-87702]

Immunohistochemistry-Paraffin: Indoleamine 2,3-dioxygenase/IDO Antibody [NBP1-87702] - Staining in human placenta and cerebral cortex tissues. Corresponding IDO1 RNA-seq data are presented for the same tissues.
Western Blot: Indoleamine 2,3-dioxygenase/IDO Antibody [NBP1-87702]

Western Blot: Indoleamine 2,3-dioxygenase/IDO Antibody [NBP1-87702]

Western Blot: Indoleamine 2,3-dioxygenase/IDO Antibody [NBP1-87702] - Analysis in human cell line EFO-21.
Immunohistochemistry-Paraffin: Indoleamine 2,3-dioxygenase/IDO Antibody [NBP1-87702]

Immunohistochemistry-Paraffin: Indoleamine 2,3-dioxygenase/IDO Antibody [NBP1-87702]

Immunohistochemistry-Paraffin: Indoleamine 2,3-dioxygenase/IDO Antibody [NBP1-87702] - Staining of human lymph node shows strong cytoplasmic and nuclear positivity in non-germinal center cells.
Immunohistochemistry-Paraffin: Indoleamine 2,3-dioxygenase/IDO Antibody [NBP1-87702]

Immunohistochemistry-Paraffin: Indoleamine 2,3-dioxygenase/IDO Antibody [NBP1-87702]

Immunohistochemistry-Paraffin: Indoleamine 2,3-dioxygenase/IDO Antibody [NBP1-87702] - Immunohistochemical staining of human placenta shows strong cytoplasmic and nuclear positivity in endothelial cells.
Immunohistochemistry-Paraffin: Indoleamine 2,3-dioxygenase/IDO Antibody [NBP1-87702]

Immunohistochemistry-Paraffin: Indoleamine 2,3-dioxygenase/IDO Antibody [NBP1-87702]

Immunohistochemistry-Paraffin: Indoleamine 2,3-dioxygenase/IDO Antibody [NBP1-87702] - Immunohistochemical staining of human cerebral cortex shows no positivity as expected.
Immunohistochemistry-Paraffin: Indoleamine 2,3-dioxygenase/IDO Antibody [NBP1-87702]

Immunohistochemistry-Paraffin: Indoleamine 2,3-dioxygenase/IDO Antibody [NBP1-87702]

Immunohistochemistry-Paraffin: Indoleamine 2,3-dioxygenase/IDO Antibody [NBP1-87702] - Immunohistochemical staining of human small intestine shows strong cytoplasmic and nuclear positivity in a subset of leukocytes.
Simple Western: Indoleamine 2,3-dioxygenase/IDO Antibody [NBP1-87702]

Simple Western: Indoleamine 2,3-dioxygenase/IDO Antibody [NBP1-87702]

Simple Western: Indoleamine 2,3-dioxygenase/IDO Antibody [NBP1-87702] - Simple Western lane view shows a specific band for IDO1 in 0.2 mg/ml of OVCAR-3 (left), h. Tonsil, raji, daudi lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Simple Western: Indoleamine 2,3-dioxygenase/IDO Antibody [NBP1-87702]

Simple Western: Indoleamine 2,3-dioxygenase/IDO Antibody [NBP1-87702]

Simple Western: Indoleamine 2,3-dioxygenase/IDO Antibody [NBP1-87702] - Electropherogram image(s) of corresponding Simple Western lane view. Indoleamine 2,3-dioxygenase/IDO antibody was used at 1:20 dilution on OVCAR-3, h. Tonsil, Raji, and Daudi lysate(s).

Applications for Indoleamine 2,3-dioxygenase/IDO Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Simple Western

1:20

Western Blot

0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in OVCAR-3, h. Tonsil, raji, daudi, separated by Size, antibody dilution of 1:20, apparent MW was 69 kDa. Separated by Size-Wes, Sally Sue/Peggy Sue.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Indoleamine 2,3-dioxygenase/IDO

Gamma-interferon (IFNG; MIM 147570) has an antiproliferative effect on many tumor cells and inhibits intracellular pathogens such as Toxoplasma and Chlamydia, at least partly because of the induction of indoleamine 2,3-dioxygenase (INDO; EC 1.13.11.42). This enzyme catalyzes the degradation of the essential amino acid L-tryptophan to N-formylkynurenine.[supplied by OMIM]

Alternate Names

3dioxygenase, IDO, IDO1, INDO, Indoleamine 2

Entrez Gene IDs

3620 (Human)

Gene Symbol

IDO1

UniProt

P14902

Additional Indoleamine 2,3-dioxygenase/IDO Products

Product Documents for Indoleamine 2,3-dioxygenase/IDO Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Indoleamine 2,3-dioxygenase/IDO Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Indoleamine 2,3-dioxygenase/IDO Antibody - BSA Free

Customer Reviews for Indoleamine 2,3-dioxygenase/IDO Antibody - BSA Free

There are currently no reviews for this product. Be the first to review Indoleamine 2,3-dioxygenase/IDO Antibody - BSA Free and earn rewards!

Have you used Indoleamine 2,3-dioxygenase/IDO Antibody - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies