Indoleamine 2,3-dioxygenase/IDO Antibody

Novus Biologicals | Catalog # NBP3-11262

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin, Western Blot, Immunoassay, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Sheep
View all Indoleamine 2,3-dioxygenase/IDO Primary Antibodies »

Product Specifications

Immunogen

The polyclonal antibody is obtained after immunization of sheep with recombinant IDO.

Specificity

Reactivity of the polyclonal antibody with IDO has been confirmed on immuno blot with IFN-gamma stimulated human peripheral blood lymphocytes.

Clonality

Polyclonal

Host

Sheep

Applications for Indoleamine 2,3-dioxygenase/IDO Antibody

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunoassay

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
For Western blotting, Immunohistochemistry and Flow Cytometry dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For Flow Cytometry, it is recommended that the polyclonal antibody is used in combination with fixing and permeabilisation.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS, 0.1% BSA

Preservative

0.02% Sodium Azide

Concentration

0.1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Indoleamine 2,3-dioxygenase/IDO

The polyclonal antibody recognizes human indoleamine 2,3-dioxygenase (IDO). IDO is an intracellular heme-containing enzyme that catalyzes the oxidative cleavage of the indole ring of several important regulatory molecules, like tryptophan, serotonin and melatonin. By doing this, IDO initiates the production of biologically active metabolites, commonly referred to as kynurenines. IDO is widely expressed in a variety of human tissues as well as in macrophages and dendritic cells (DCs). In inflammation, interferons (IFNs) act on specific receptors to trigger IDO induction. The production of IFN-gamma and induction of IDO represent important antimicrobial mechanisms. Degradation and depletion of tryptophan by IDO inhibits the growth of viruses, bacteria and parasites. Furthermore, IDO plays a complex and crucial role in immunoregulation during infection, pregnancy, autoimmunity, transplantation, and neoplasia. By local depletion of tryptophan and increasing proapoptotic kynurenines, IDO greatly affects T-cell proliferation and survival, both in vitro and in vivo, and also affects B-cell and NK-cell function and survival. There is a central role for IDO expression in tolerance involving regulatory cells and DCs. IDO acts as an intermediate pathway in LPS-induced production of reactive oxygen species and NF-kappaB activation, two processes that lead to DC maturation. The polyclonal antibody is obtained after immunization of sheep with recombinant IDO. Reactivity of the polyclonal antibody with IDO has been confirmed on immuno blot with IFN-gamma stimulated human peripheral blood lymphocytes.

Alternate Names

3dioxygenase, IDO, IDO1, INDO, Indoleamine 2

Gene Symbol

IDO1

Additional Indoleamine 2,3-dioxygenase/IDO Products

Product Documents for Indoleamine 2,3-dioxygenase/IDO Antibody

Certificate of Analysis

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Product Specific Notices for Indoleamine 2,3-dioxygenase/IDO Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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