Infectious Bronchitis Virus Antibody (IB95) - BSA Free

Novus Biologicals | Catalog # NBP2-31102

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Virus

Cited:

Virus

Applications

Validated:

Western Blot, Sandwich ELISA, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # IB95

Format

BSA Free
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Product Specifications

Immunogen

Hybridoma clone has been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with semi-purified inactivated Infectious Bronchitis virus of Massachusetts type from infected chicken embryos.

Specificity

Nucleoprotein of IBV

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Infectious Bronchitis Virus Antibody (IB95) - BSA Free

Infectious Bronchitis Virus Antibody (IB95) - BSA Free

Western Blot: Infectious Bronchitis Virus Antibody (IB95) - BSA Free [NBP2-31102] -

Investigating the regulatory function of chJMJD6 in IBV infection.A Quantitative analysis of chJMJD6 overexpression vector efficiency using indirect immunofluorescence. B Changes of chJMJD6 protein expression at different time points of IBV infection. C Time-course analysis of IBV replication upon chJMJD6 overexpression. D Protein expression analysis of IBV N protein following 36 h post-chJMJD6 overexpression. E The change in TCID50 of HD11 supernatant after infection with IBV following overexpression of chJMJD6; F–G Identification of critical chJMJ6 interference points through targeted siRNA interference screens; H Monitoring the effect of siRNA-mediated reduction of chJMJD6 expression on IBV-N mRNA levels at different time points post-infection. I Protein level assessment of IBV N by 36 hpi in siRNA-chJMJD6 knockdown samples. J The change in TCID50 of HD11 supernatant after infection with IBV following knockdown of chJMJD6 with siRNA. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/39501382), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for Infectious Bronchitis Virus Antibody (IB95) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Immunodetection of IBV N-protein in Western Blotting; IB virus detection in sandwich-type immunoassay (capture). Use in ICC/IF reported in scientific literature (PMID:33472590)

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Product Documents for Infectious Bronchitis Virus Antibody (IB95) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Infectious Bronchitis Virus Antibody (IB95) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Infectious Bronchitis Virus Antibody (IB95) - BSA Free

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Infectious Bronchitis Virus Antibody (IB95) - BSA Free

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  • Q: What is the concentration of this 1mg preparation?

    A: The concentration of this product varies from lot to lot. The lot specific concentration will be written in the vial label as well as the data sheet which is shipped together with each vial. For example for the current lot of #3BN1-IB95, the concentration is 6.0 mg/ml. The purity of all our antibodies in general is >92%

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