Influenza A H1N1 Nucleoprotein Antibody (GT1236) - (A/WSN/1933) - Azide and BSA Free
Novus Biologicals | Catalog # NBP3-13493
![Western Blot: Influenza A H1N1 Nucleoprotein Antibody (GT1236)(A/WSN/1933) [NBP3-13493]](https://resources.rndsystems.com/images/products/Influenza-A-nucleoprotein-Antibody-GT1236-Western-Blot-NBP3-13493-img0005.jpg "Western Blot: Influenza A H1N1 Nucleoprotein Antibody (GT1236)(A/WSN/1933) [NBP3-13493]")
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Key Product Details
Species Reactivity
Virus
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Sandwich ELISA, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # GT1236
Format
Azide and BSA Free
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Product Specifications
Immunogen
Recombinant protein of Influenza A H1N1 Nucleoprotein (A/WSN/1933(H1N1). The exact sequence is proprietary.
Specificity
Influenza A H1N1 Nucleoprotein. (A/WSN/1933)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Description
Centrifuge briefly prior to opening.
Scientific Data Images
Western Blot: Influenza A H1N1 Nucleoprotein Antibody (GT1236)(A/WSN/1933) [NBP3-13493]
Western Blot: Influenza A H1N1 Nucleoprotein Antibody (GT1236) [NBP3-13493] - Influenza A H1N1 Nucleoprotein antibody [GT1236] detects Influenza A H1N1 Nucleoprotein by Western blot analysis. A. 5 ug DF1 whole cell lysate/extract B. 5 ug whole cell lysate/extract of WSN virus infected DF1 cells (10 hr) 7.5 % SDS-PAGE Influenza A H1N1 Nucleoprotein antibody [GT1236] (NBP3-13493) dilution: 1:5000![Immunocytochemistry/ Immunofluorescence: Influenza A H1N1 Nucleoprotein Antibody (GT1236) - (A/WSN/1933) [NBP3-13493]](https://resources.rndsystems.com/images/products/Influenza-A-nucleoprotein-Antibody-GT1236-Immunocytochemistry-Immunofluorescence-NBP3-13493-img0004.jpg "Immunocytochemistry/ Immunofluorescence: Influenza A H1N1 Nucleoprotein Antibody (GT1236) - (A/WSN/1933) [NBP3-13493]")
Immunocytochemistry/ Immunofluorescence: Influenza A H1N1 Nucleoprotein Antibody (GT1236) - (A/WSN/1933) [NBP3-13493]
Immunocytochemistry/Immunofluorescence: Influenza A H1N1 Nucleoprotein Antibody (GT1236) [NBP3-13493] - Influenza A H1N1 Nucleoprotein antibody [GT1236] detects Influenza A H1N1 Nucleoprotein by immunofluorescent analysis. Sample: DF1 cells infected with Influenza A virus (WSN). Green: Influenza A H1N1 Nucleoprotein stained by Influenza A H1N1 Nucleoprotein antibody [GT1236] (NBP3-13493) diluted at 1:500. Blue: Hoechst 33342 staining.
Sandwich ELISA: Influenza A H1N1 Nucleoprotein Antibody (GT1236) [NBP3-13493] - Sandwich ELISA detection of non-transfected and Influenza A virus nucleoprotein transfected 293T whole cell extracts using Influenza A H1N1 Nucleoprotein antibody [GT1236] (NBP3-13493) as capture antibody at concentration of 5 ug/mL and Influenza A H1N1 Nucleoprotein antibody (NBP2-16965) as detection antibody at concentration of 1 ug/mL. Rabbit IgG antibody (HRP) (NBP2-19301) was diluted at 1:10000 and used to detect the primary antibody.
Sandwich ELISA: Influenza A H1N1 Nucleoprotein Antibody (GT1236) [NBP3-13493] - Sandwich ELISA detection of recombinant full-length Influenza A H1N1 Nucleoprotein, DDDDK tag using Influenza A H1N1 Nucleoprotein antibody (NBP2-16965) as capture antibody at concentration of 5 ug/mL and Influenza A H1N1 Nucleoprotein antibody [GT1236] (NBP3-13493) as detection antibody at concentration of 1 ug/mL. Mouse IgG antibody (HRP) (NBP2-19382) was diluted at 1:10000 and used to detect the primary antibody.
Sandwich ELISA: Influenza A H1N1 Nucleoprotein Antibody (GT1236) [NBP3-13493] - Sandwich ELISA detection of recombinant full-length Influenza A H1N1 Nucleoprotein, DDDDK tag using Influenza A H1N1 Nucleoprotein antibody [GT1236] (NBP3-13493) as capture antibody at concentration of 5 ug/mL and Influenza A H1N1 Nucleoprotein antibody (NBP2-16965) as detection antibody at concentration of 1 ug/mL. Rabbit IgG antibody (HRP) (NBP2-19301) was diluted at 1:10000 and used to detect the primary antibody.
Western Blot: Influenza A H1N1 Nucleoprotein Antibody (GT1236) - (A/WSN/1933) [NBP3-13493] -
Western Blot: Influenza A H1N1 Nucleoprotein Antibody (GT1236) - (A/WSN/1933) [NBP3-13493] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Influenza A H1N1 Nucleoprotein antibody [GT1236] (NBP3-13493) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
ELISA: Influenza A H1N1 Nucleoprotein Antibody (GT1236) - (A/WSN/1933) [NBP3-13493] -
Sandwich ELISA detection of recombinant Influenza A virus Nucleoprotein (A/WSN/1933 (H1N1)), DDDDK Tag using antibodies as below.Capture: Influenza A virus Nucleoprotein antibody [HL4098] (5 ug/mL)
Detection: Influenza A virus Nucleoprotein antibody [GT1236] (NBP3-13493) (1 ug/mL)
ELISA: Influenza A H1N1 Nucleoprotein Antibody (GT1236) - (A/WSN/1933) [NBP3-13493] -
Sandwich ELISA detection of recombinant Influenza A virus Nucleoprotein (A/WSN/1933 (H1N1)), DDDDK Tag using antibodies as below.Capture: Influenza A virus Nucleoprotein antibody [HL1103] (5 ug/mL)
Detection: Influenza A virus Nucleoprotein antibody [GT1236] (NBP3-13493) (1 ug/mL)
ELISA: Influenza A H1N1 Nucleoprotein Antibody (GT1236) - (A/WSN/1933) [NBP3-13493] -
Sandwich ELISA detection of recombinant Influenza A virus Nucleoprotein (A/WSN/1933 (H1N1)), DDDDK Tag using antibodies as below.Capture: Influenza A virus Nucleoprotein antibody [HL1089] (5 ug/mL)
Detection: Influenza A virus Nucleoprotein antibody [GT1236] (NBP3-13493) (1 ug/mL)
ELISA: Influenza A H1N1 Nucleoprotein Antibody (GT1236) - (A/WSN/1933) [NBP3-13493] -
Sandwich ELISA detection of recombinant Influenza A virus Nucleoprotein (A/WSN/1933 (H1N1)), DDDDK Tag using antibodies as below.Capture: Influenza A virus Nucleoprotein antibody [HL1098] (5 ug/mL)
Detection: Influenza A virus Nucleoprotein antibody [GT1236] (NBP3-13493) (1 ug/mL)
Immunocytochemistry/ Immunofluorescence: Influenza A H1N1 Nucleoprotein Antibody (GT1236) - (A/WSN/1933) [NBP3-13493] -
Influenza A virus Nucleoprotein antibody [GT1236] detects Influenza A virus Nucleoprotein protein by immunofluorescent analysis.Sample: Mock and transfected 293T cells were fixed in ice-cold MeOH for 5 min.
Green: Influenza A virus Nucleoprotein stained by Influenza A virus Nucleoprotein antibody [GT1236] (NBP3-13493) diluted at 1:500.
Blue: Fluoroshield with DAPI.
Applications
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Sandwich ELISA
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Product Documents
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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