Influenza A H1N1 Nucleoprotein Antibody - BSA Free

Novus Biologicals | Catalog # NB100-56570

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Influenza A Virus H1N1, Virus

Cited:

Hamster, Influenza A Virus H1N1

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA

Cited:

Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

Recombinant Influenza A H1N1 Nucleoprotein.

Specificity

Influenza A virus (A/Puerto Rico/8/34/Mount Sinai (H1N1) segment 5) nuclear protein.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Influenza A H1N1 Nucleoprotein Antibody - BSA Free

Immunohistochemistry: Influenza A H1N1 Nucleoprotein Antibody [NB100-56570]

Immunohistochemistry: Influenza A H1N1 Nucleoprotein Antibody [NB100-56570]

Influenza-A-H1N1-Nucleoprotein-Antibody-Immunohistochemistry-NB100-56570-img0006.jpg
Western Blot: Influenza A H1N1 Nucleoprotein Antibody [NB100-56570]

Western Blot: Influenza A H1N1 Nucleoprotein Antibody [NB100-56570]

Western Blot: Influenza A H1N1 Nucleoprotein Antibody [NB100-56570] - analysis of Influenza A in 50 ngs of recombinant protein using this antibody.
ELISA: Influenza A H1N1 Nucleoprotein Antibody [NB100-56570]

ELISA: Influenza A H1N1 Nucleoprotein Antibody [NB100-56570]

ELISA: Influenza A H1N1 Nucleoprotein Antibody [NB100-56570] - Influenza A standard curve. Influenza A monclonal antibody, clone 2F205 was used as the capture antibody. Influenza A polyclonal antibody (NB100-56570) was used as the detection antibody. Recombinant Influenza A Nucleoprotein was used as the protein standard. Researchers should empirically determine the optimal antibody concentrations for their ELISA assays.
Influenza A H1N1 Nucleoprotein Antibody

Immunocytochemistry/ Immunofluorescence: Influenza A H1N1 Nucleoprotein Antibody [NB100-56570] -

Immunocytochemistry/ Immunofluorescence: Influenza A H1N1 Nucleoprotein Antibody [NB100-56570] - CA/07 & NL/602 induced qualitatively similar expression of nucleoprotein in airway epithelial cells but distinct recruitment of F4/80+ cells & CD11b+ cells.Frozen lung sections collected two (B) & four (C) days post infection were stained with anti-influenza nucleoprotein (blue), anti-F4/80 antigen (green) or anti-CD11b (red). Both CA/07 & NL/602 induced expression of viral nucleoprotein prominently on bronchiole epithelial cells & modest expression on alveolar epithelial cells. F4/80+ & CD11b+ cells were detected in greater abundance upon infection with NL/602 compared to naïve lung. In contrast, CA/07 infection yielded substantially greater recruitment of CD11b+ cells while F4/80+ cells were minimally detected. Insets from CA/07 & NL/602 shown in 40X objective (D). Scale bars represent 100µm. Image collected & cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0076708), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Influenza A H1N1 Nucleoprotein Antibody

Immunocytochemistry/ Immunofluorescence: Influenza A H1N1 Nucleoprotein Antibody [NB100-56570] -

Immunocytochemistry/ Immunofluorescence: Influenza A H1N1 Nucleoprotein Antibody [NB100-56570] - CA/07 & NL/602 induced qualitatively similar expression of nucleoprotein in airway epithelial cells but distinct recruitment of F4/80+ cells & CD11b+ cells.Frozen lung sections collected two (B) & four (C) days post infection were stained with anti-influenza nucleoprotein (blue), anti-F4/80 antigen (green) or anti-CD11b (red). Both CA/07 & NL/602 induced expression of viral nucleoprotein prominently on bronchiole epithelial cells & modest expression on alveolar epithelial cells. F4/80+ & CD11b+ cells were detected in greater abundance upon infection with NL/602 compared to naïve lung. In contrast, CA/07 infection yielded substantially greater recruitment of CD11b+ cells while F4/80+ cells were minimally detected. Insets from CA/07 & NL/602 shown in 40X objective (D). Scale bars represent 100µm. Image collected & cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0076708), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Influenza A H1N1 Nucleoprotein Antibody

Immunocytochemistry/ Immunofluorescence: Influenza A H1N1 Nucleoprotein Antibody [NB100-56570] -

Immunocytochemistry/ Immunofluorescence: Influenza A H1N1 Nucleoprotein Antibody [NB100-56570] - CA/07 & NL/602 induced qualitatively similar expression of nucleoprotein in airway epithelial cells but distinct recruitment of F4/80+ cells & CD11b+ cells.Frozen lung sections collected two (B) & four (C) days post infection were stained with anti-influenza nucleoprotein (blue), anti-F4/80 antigen (green) or anti-CD11b (red). Both CA/07 & NL/602 induced expression of viral nucleoprotein prominently on bronchiole epithelial cells & modest expression on alveolar epithelial cells. F4/80+ & CD11b+ cells were detected in greater abundance upon infection with NL/602 compared to naïve lung. In contrast, CA/07 infection yielded substantially greater recruitment of CD11b+ cells while F4/80+ cells were minimally detected. Insets from CA/07 & NL/602 shown in 40X objective (D). Scale bars represent 100µm. Image collected & cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0076708), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for Influenza A H1N1 Nucleoprotein Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunohistochemistry

reported in scientific literature (PMID 24086762)

Immunohistochemistry-Paraffin

reported in scientific literature (PMID 16873262)

Western Blot

1-3 ug/ml

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Influenza A H1N1 Nucleoprotein

Additional Influenza A H1N1 Nucleoprotein Products

Product Documents for Influenza A H1N1 Nucleoprotein Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Influenza A H1N1 Nucleoprotein Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Influenza A H1N1 Nucleoprotein Antibody - BSA Free

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Protocols

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