iNOS Antibody (K13-A)

Novus Biologicals | Catalog # NBP1-33780

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine

Cited:

Human, Mouse, Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence, IF/IHC, Knockdown Validated

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # K13-A
View all iNOS Primary Antibodies »

Product Specifications

Immunogen

Peptide derived from human iNOS sequence. Antibody recognizes the epitope between Ser1118 - Gly1129.

Epitope

Ser1118 - Gly1129

Reactivity Notes

This antibody also recognizes nNOS (neuronal nitric oxide synthase). Porcine reactivity reported per verified customer review.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody is immunoaffinity purified with immunogenic peptide as a ligand.

Scientific Data Images for iNOS Antibody (K13-A)

Immunohistochemistry-Paraffin: iNOS Antibody (K13-A) [NBP1-33780]

Immunohistochemistry-Paraffin: iNOS Antibody (K13-A) [NBP1-33780]

Immunohistochemistry-Paraffin: iNOS Antibody (K13-A) [NBP1-33780] - Liver tissue stained with anti-iNOS antibody shows strong positive immunostaining of hepatocytes. Formalin fixed, paraffin embedded human tissues (4 um sections) stained according to IHC-P Protocol for NBP1-33780.
Immunohistochemistry-Paraffin: iNOS Antibody (K13-A) [NBP1-33780]

Immunohistochemistry-Paraffin: iNOS Antibody (K13-A) [NBP1-33780]

Immunohistochemistry-Paraffin: iNOS Antibody (K13-A) [NBP1-33780] - Lung adenocarcinoma tissue stained with anti-iNOS antibody shows strong positive immunostaining of hepatocytes. Formalin fixed, paraffin embedded human tissues (4 um sections) stained according to IHC-P Protocol for NBP1-33780.
Immunohistochemistry-Paraffin: iNOS Antibody (K13-A) [NBP1-33780]

Immunohistochemistry-Paraffin: iNOS Antibody (K13-A) [NBP1-33780]

Immunohistochemistry-Paraffin: iNOS Antibody (K13-A) [NBP1-33780] - Inflamed porcine intestine. Antibody dilution 1:200, overnight 4C. Secondary anti-rabbit Alexa 488 (1:1000) 2 hr incubation. Image from verified customer review.
iNOS Antibody (K13-A)

Western Blot: iNOS Antibody (K13-A) [NBP1-33780] -

Effects of omega-3 supplementation on the immunocontent of macrophages M1 markers: nitric oxide synthase 2 (NOS-2) (A) and CD86 (B), and M2 marker: Arginase-1 (C) on the mouse paw muscle 48 h after the model induction in Saline/Sham, Saline/CPIP, Omega-3/CPIP, and Corn oil/CPIP groups. Data are expressed as mean ± SD of 6 animals per group, statistically assessed by the one-way ANOVA followed by Tukey’s test. ##p < 0.01 vs. the Saline/Sham group.
iNOS Antibody (K13-A)

Western Blot: iNOS Antibody (K13-A) [NBP1-33780] -

Western Blot: iNOS Antibody (K13-A) [NBP1-33780] - Effects of omega-3 supplementation on the immunocontent of macrophages M1 markers: nitric oxide synthase 2 (NOS-2) (A) & CD86 (B), & M2 marker: Arginase-1 (C) on the mouse paw muscle 48 h after the model induction in Saline/Sham, Saline/CPIP, Omega-3/CPIP, & Corn oil/CPIP groups. Data are expressed as mean ± SD of 6 animals per group, statistically assessed by the one-way ANOVA followed by Tukey’s test. ##p < 0.01 vs. the Saline/Sham group. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/35391753), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for iNOS Antibody (K13-A)

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:100-1:200

Western Blot

1:2000
Application Notes
Use in IHC-P reported in scientific literature (PMID:34496231).

Reviewed Applications

Read 1 review rated 5 using NBP1-33780 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

20mM Tris-HCl (pH 8.0) and 20mg/ml BSA

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: iNOS

Nitric oxide (NO) is a colorless, free radical gas that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family including the two constitutive isoforms: brain, bNOS, or neuronal NOS, nNOS (type I) and endothelial cell NOS, eNOS (type III); along with the inducible isoform, iNOS (type II). NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO, requiring the cofactors calmodulin, nicotinamide adenine dinucleotide phosphate (NADPH), flavin adenine dinucleotide (FAD), and flavin mononucleotide (FMN), heme, and tetrahydrobiopterin (1).

The 131 kDa enzyme, iNOS, is found in a variety of cell types including macrophages, hepatocytes, synoviocytes, and smooth muscle cells. While constitutively expressed in kidneys, in other tissues iNOS is induced by bacterial lipopolysaccharides (LPS), growth factors, and cytokines such as IFN-gamma, TNF, IL-1 and IL-2. iNOS is not regulated by the level of intracellular Ca2+ and is constantly active as a dimer when expressed. iNOS activity is elevated in a variety of diseases including atherosclerosis, heart failure, sepsis, solid tumors, and type 2 diabetes. Acting as a critical mediator of inflammation and apoptosis, iNOS inhibitors have been shown to alleviate obesity and stress inducted insulin resistance in mouse models (2,3).

References

1. Forstermann U, and Sessa WC. (2012) Nitric oxide synthases: regulation and function. Eur Heart J. 33(7): 829-837. PMID: 21890489

2. Aktan F. (2004) iNOS-mediated nitric oxide production and its regulation. Life Sci. 75(6):639-53. PMID: 15172174

3. Cinelli MA, Do HT, Miley GP, Silverman RB. (2020) Inducible nitric oxide synthase: Regulation, structure, and inhibition. Med Res Rev. 40(1):158-189. PMID: 31192483

Long Name

Inducible Nitic Oxide Synthase

Alternate Names

NOS2, NOS2A

Entrez Gene IDs

4843 (Human)

Gene Symbol

NOS2

UniProt

P35228

Additional iNOS Products

Product Documents for iNOS Antibody (K13-A)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for iNOS Antibody (K13-A)

This antibody is immunoaffinity purified with immunogenic peptide as a ligand.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for iNOS Antibody (K13-A)

Customer Reviews for iNOS Antibody (K13-A) (1)

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  • iNOS Antibody (K13-A)
    Name: Sandra Lourenssen
    Application: Immunohistochemistry-Paraffin
    Sample Tested: paraffin sections of inflamed intestine
    Species: Porcine
    Verified Customer | Posted 04/18/2018
    Antibody dilution 1:200, overnight 4C. Secondary anti-rabbit Alexa 488 (1:1000) 2 hr incubation.
    iNOS Antibody (K13-A) NBP1-33780

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Protocols

View specific protocols for iNOS Antibody (K13-A) (NBP1-33780):

Immunohistochemistry-Paraffin protocol for iNOS Antibody (NBP1-33780):
Immunohistochemistry-Paraffin

1. Deparaffinize the section in 3 changes of xylene, 5 minutes each.
2. Wash the section in 96%, 80% and 70% ethyl alcohol for 10 minutes each.
3. Rinse in distilled water.
4. Block the endogenous peroxidase by incubating the tissue in 3% hydrogen peroxide (H2O2) for 10 minutes.
5. Wash in distilled water.
6. For antigen retrieval: Immerse the slide in Tris-EDTA buffer*, pH 9.0 and incubate at 95-97C in water bath for 25 minutes. (Alternatively adjust to your own protocol, keeping the required pH)
7. Remove the staining to room temperature and let the slide to cool (in Tris-EDTA buffer, pH 9.0) for 15 minutes.
8. Rinse in distilled water, 2 x 5 minutes.
9. Wash in PBS (phosphate buffer saline, pH 7.0-7.5) supplemented with 0.05% of Tween-20 (buffer A), 2 x 5 minutes.
10. Incubate the section with primary antibody at the dilution 1:100 - 1:200 for 1 hour in the closed wet chamber.
11. Wash 3 x 5 minutes with buffer A.
12. Apply the secondary antibody (the protocol depends on the supplier), and proceed with immunohistochemistry protocol (HRP - Peroxide - DAB).
13. Wash 3 x 5 minutes with buffer A.
14. Apply the chromogen (DAB), 1-3 minutes.
15. Wash in water, 2 x 5 minutes.
16. Stain in hematoxylin for 5 minutes.
17. Wash in water, 2 x 5 minutes.
18. Stain in hematoxylin for 5 minutes.
19. Wash in distilled water, 3 x 2 minutes.
20. Mount the slide for observation.

* Tris-EDTA Buffer (10mM Tris Base, 1mM EDTA solution, pH 9.0)
Tris -- 1.21 g; EDTA -- 0.37 g; Distilled water -- 1000 ml
Mix to dissolve in 700 ml of distilled water. Adjust pH to 9.0 with 1M HCl and mix well.
Adjust the final volume to 1 liter with distilled water.
Store this solution at room temperature for 3 months or at 4C for longer storage.

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for iNOS Antibody (K13-A)

Showing  1 - 3 of 3 FAQs Showing All

  • Q: Hi, I would like to know if this antibody (NBP1-33780) works on mouse tissue in Immunohistochemistry. Did you test the mouse cross-reactivity on IHC ?

    A: At this time we have only tested NBP1-33780 on human tissues in IHC, but we do expect it to work on mouse tissues in IHC based on sequence homology.

  • Q: We need to evaluate the level of iNOS in Rat sciatic nerve by Western Blot. I see that you have few antibodies suitable for that, could you please recommend the best what you have for our purposes?

    A: We have 4 iNOS antibodies that are suitable for your experiment. The best choice for you will depend on your specific preferences, in this case the main difference will be the immunogen location. If the immunogen is not important to you, then I would recommend choosing NB300-605 as it gives you the best value and we are able to provide the exact immunogen.

  • Q: What is the concentration of NBP1-33780?

    A: The concentration of the lot number DB003-02-5B is 4.9 mg/ml

  • Q: Hi, I would like to know if this antibody (NBP1-33780) works on mouse tissue in Immunohistochemistry. Did you test the mouse cross-reactivity on IHC ?

    A: At this time we have only tested NBP1-33780 on human tissues in IHC, but we do expect it to work on mouse tissues in IHC based on sequence homology.

  • Q: We need to evaluate the level of iNOS in Rat sciatic nerve by Western Blot. I see that you have few antibodies suitable for that, could you please recommend the best what you have for our purposes?

    A: We have 4 iNOS antibodies that are suitable for your experiment. The best choice for you will depend on your specific preferences, in this case the main difference will be the immunogen location. If the immunogen is not important to you, then I would recommend choosing NB300-605 as it gives you the best value and we are able to provide the exact immunogen.

  • Q: What is the concentration of NBP1-33780?

    A: The concentration of the lot number DB003-02-5B is 4.9 mg/ml

  • Q: Hi, I would like to know if this antibody (NBP1-33780) works on mouse tissue in Immunohistochemistry. Did you test the mouse cross-reactivity on IHC ?

    A: At this time we have only tested NBP1-33780 on human tissues in IHC, but we do expect it to work on mouse tissues in IHC based on sequence homology.

  • Q: We need to evaluate the level of iNOS in Rat sciatic nerve by Western Blot. I see that you have few antibodies suitable for that, could you please recommend the best what you have for our purposes?

    A: We have 4 iNOS antibodies that are suitable for your experiment. The best choice for you will depend on your specific preferences, in this case the main difference will be the immunogen location. If the immunogen is not important to you, then I would recommend choosing NB300-605 as it gives you the best value and we are able to provide the exact immunogen.

  • Q: What is the concentration of NBP1-33780?

    A: The concentration of the lot number DB003-02-5B is 4.9 mg/ml

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