iNOS Antibody (PSH03-69)

Western Blot: iNOS Antibody (PSH03-69) [NBP3-32473]

Western Blot: iNOS Antibody (PSH03-69) [NBP3-32473] - Western blot analysis of iNOS on different lysates with Rabbit anti-iNOS antibody (NBP3-32473) at 1/1,000 dilution.

Lane 1: RAW264.7 cell lysate
Lane 2: RAW264.7 treated with 1ug/mL LPS for 24 hours cell lysate

Lysates/proteins at 10 ug/Lane.

Predicted band size: 131 kDa
Observed band size: 131 kDa

Exposure time: 3 minutes; ECL;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32473) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: iNOS Antibody (PSH03-69) [NBP3-32473]

Immunohistochemistry: iNOS Antibody (PSH03-69) [NBP3-32473] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Rabbit anti-iNOS antibody (NBP3-32473) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32473) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunocytochemistry/ Immunofluorescence: iNOS Antibody (PSH03-69) [NBP3-32473]

Immunocytochemistry/ Immunofluorescence: iNOS Antibody (PSH03-69) [NBP3-32473] - Immunocytochemistry analysis of RAW264.7 cells treated with or without 1μg/mL LPS for 6 hours labeling iNOS with Rabbit anti-iNOS antibody (NBP3-32473) at 1/100 dilution.

Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-iNOS antibody (NBP3-32473) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.