Insulin Antibody (E2-E3 + 2D11-H5 (same as INS04 + INS05)) - IHC-Prediluted

Novus Biologicals | Catalog # NBP2-45107

Novus Biologicals

Key Product Details

Species Reactivity

Human, Mouse, Porcine, Bovine, Rabbit

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Kappa/IgG1 Kappa Clone # E2-E3 + 2D11-H5 (same as INS04 + INS05)

Format

IHC-Prediluted
View all Insulin Primary Antibodies »

Product Specifications

Immunogen

Purified pig insulin, conjugated to KLH (E2-E3 & 2D11-H5) (Uniprot: P01308)

Localization

Cytoplasmic

Marker

beta-Cell & Insulinoma Marker

Specificity

Recognizes a polypeptide which is identified as insulin, a 51-amino acid polypeptide composed of A and B chains connected through the C-peptide. Proinsulin, which has very little biological activity, is cleaved by proteases within its cell of origin into the insulin molecule and the C-terminal basic residue. Insulin enhances membrane transport of glucose, amino acids, and certain ions. It also promotes glycogen storage, formation of triglycerides, and synthesis of proteins and nucleic acids. Deficiency of insulin results in diabetes mellitus. The main storage site for insulin is the pancreatic islets. Antibodies to insulin are important as beta-cell and insulinoma marker.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 Kappa/IgG1 Kappa

Theoretical MW

6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

The prediluted antibody does not require any mixing, dilution, reconstitution, or titration; the antibody is ready-to-use and optimized for staining.

Scientific Data Images

Immunohistochemistry-Paraffin: Insulin Antibody (E2-E3 + 2D11-H5 (same as INS04 + INS05)) - IHC-Prediluted [NBP2-45107]

Immunohistochemistry-Paraffin: Insulin Antibody (E2-E3 + 2D11-H5 (same as INS04 + INS05)) - IHC-Prediluted [NBP2-45107]

Immunohistochemistry-Paraffin: Insulin Antibody (E2-E3 + 2D11-H5 (same as INS04 + INS05)) - IHC-Prediluted [NBP2-45107] - Formalin-fixed, paraffin-embedded human pancreas stained with insulin Monoclonal Antibody (E2-E3+2D11-H5).
Immunohistochemistry-Paraffin: Insulin Antibody (E2-E3 + 2D11-H5 (same as INS04 + INS05)) - IHC-Prediluted [NBP2-45107]

Immunohistochemistry-Paraffin: Insulin Antibody (E2-E3 + 2D11-H5 (same as INS04 + INS05)) - IHC-Prediluted [NBP2-45107]

Immunohistochemistry-Paraffin: Insulin Antibody (E2-E3 + 2D11-H5 (same as INS04 + INS05)) - IHC-Prediluted [NBP2-45107] - Adult human pancreas. Antibody 1:200; heat-induced epitope retrieval with citrate buffer. Image from verified customer review.

Applications

Application
Recommended Usage

Immunohistochemistry-Paraffin

0.1 - 0.2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

10 mM PBS with 0.05% BSA

Format

IHC-Prediluted

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Insulin

After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into two chains (peptide A and peptide B) that are covalently linked via two disulfide bonds. Binding of this mature form of insulin to the insulin receptor (INSR) stimulates glucose uptake. A variety of mutant alleles with changes in the coding region have been identified. There is a read-through gene, INS-IGF2, which overlaps with this gene at the 5' region and with the IGF2 gene at the 3' region. (provided by RefSeq)

Alternate Names

IDDM2, ILPR, INS, IRDN, MODY10

Gene Symbol

INS

Additional Insulin Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

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    • Q: Will you please recommend me antibodies to insulin, the most suitable for immunohistochemistry in the pancreas of guinea pigs? Desirable to be conjugated with some pigment and there was no need for secondary antibodies.
      A: I have carried out a search and the best antibody I recommend is NB600-1488. This is a primary unconjugated antibody however and you will need a secondary. If you click on the product link and then click on related products, a list of all recommended secondary antibodies will appear.
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