Insulin Antibody

Novus Biologicals | Catalog # NBP1-45662

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Rat

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all Insulin Primary Antibodies »

Product Specifications

Immunogen

E. coli expressed recombinant human Insulin.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This product is manufactured by Abcam and distributed by Novus Biologicals. (Abcam Catalog Number: ab286162)

Scientific Data Images for Insulin Antibody

Western Blot: Insulin Antibody [NBP1-45662]

Western Blot: Insulin Antibody [NBP1-45662]

Western Blot: Insulin Antibody [NBP1-45662] - Analysis using recombinant human Insulin.

Applications for Insulin Antibody

Application
Recommended Usage

Western Blot

0.5 - 4 ug/mL

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2), 0.5% BSA, 30% glycerol

Preservative

0.01% Thimerosal

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Insulin

Insulin is one of the major regulatory hormones of intermediate metabolism throughout the body. The biological actions of this hormone involve integration of carbohydrate, protein, and lipid metabolism. Insulin enhances membrane transport of glucose, amino acids, and certain ions. It also promotes glycogen storage, formation of triglycerides and synthesis of proteins and nucleic acids. Immunocytochemical investigations have localized insulin in the B cells of pancreatic islets of Langerhans. Deficiency of insulin results in diabetes mellitus, one of the leading causes of morbidity and mortality in the general population. Insulin is also present in tumors of B cell origin such as insulinoma.

Alternate Names

IDDM2, ILPR, INS, IRDN, MODY10

Entrez Gene IDs

3630 (Human)

Gene Symbol

INS

UniProt

P01308

Additional Insulin Products

Product Documents for Insulin Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Insulin Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Citations for Insulin Antibody

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FAQs for Insulin Antibody

Showing  1 - 2 of 2 FAQs Showing All

  • Q: Were special non-denaturing and/or non-reducing conditions used to obtain the WB image for your insulin Ab? The band you show would suggest that the insulin di-peptide is intact (disulfides).

    A: Our WB image for NBP1-45662 was generated from testing performed on a partial recombinant insulin protein. The protein is 50 amino acids in length, which corresponds to the ~5.5kDa observed weight.

  • Q: Will you please recommend me antibodies to insulin, the most suitable for immunohistochemistry in the pancreas of guinea pigs? Desirable to be conjugated with some pigment and there was no need for secondary antibodies.

    A:

    I have carried out a search and the best antibody I recommend is NB600-1488. This is a primary unconjugated antibody however and you will need a secondary. If you click on the product link and then click on related products, a list of all recommended secondary antibodies will appear.

  • Q: Were special non-denaturing and/or non-reducing conditions used to obtain the WB image for your insulin Ab? The band you show would suggest that the insulin di-peptide is intact (disulfides).

    A: Our WB image for NBP1-45662 was generated from testing performed on a partial recombinant insulin protein. The protein is 50 amino acids in length, which corresponds to the ~5.5kDa observed weight.

  • Q: Will you please recommend me antibodies to insulin, the most suitable for immunohistochemistry in the pancreas of guinea pigs? Desirable to be conjugated with some pigment and there was no need for secondary antibodies.

    A:

    I have carried out a search and the best antibody I recommend is NB600-1488. This is a primary unconjugated antibody however and you will need a secondary. If you click on the product link and then click on related products, a list of all recommended secondary antibodies will appear.

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