Integrin alpha 6/CD49f Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-85747

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Simple Western

Cited:

Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Integrin alpha 6/CD49f Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: APYDDLGKVFIYHGSANGINTKPTQVLKGISPYFGYSIAGNMDLDRNSYPDVAVGSLSDSVTIFRSRPVINIQKTITVTPNRIDLRQKTACGAPSGICLQVKSCFEYTANPAGYNPSISIVGTLEAEKERRKSGLSS

Reactivity Notes

Rat (86%), Mouse (88%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

127 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Integrin alpha 6/CD49f Antibody - BSA Free

Immunohistochemistry-Paraffin: Integrin alpha 6/CD49f Antibody [NBP1-85747]

Immunohistochemistry-Paraffin: Integrin alpha 6/CD49f Antibody [NBP1-85747]

Immunohistochemistry-Paraffin: Integrin alpha 6/CD49f Antibody [NBP1-85747] - Staining in human placenta and liver tissues. Corresponding ITGA6 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: Integrin alpha 6/CD49f Antibody [NBP1-85747]

Immunohistochemistry-Paraffin: Integrin alpha 6/CD49f Antibody [NBP1-85747]

Immunohistochemistry-Paraffin: Integrin alpha 6/CD49f Antibody [NBP1-85747] - Staining of human kidney shows moderate membranous positivity in cells in tubules.
Immunohistochemistry-Paraffin: Integrin alpha 6/CD49f Antibody [NBP1-85747]

Immunohistochemistry-Paraffin: Integrin alpha 6/CD49f Antibody [NBP1-85747]

Immunohistochemistry-Paraffin: Integrin alpha 6/CD49f Antibody [NBP1-85747] - Staining of human liver shows no positivity in hepatocytes as expected.
Immunohistochemistry-Paraffin: Integrin alpha 6/CD49f Antibody [NBP1-85747]

Immunohistochemistry-Paraffin: Integrin alpha 6/CD49f Antibody [NBP1-85747]

Immunohistochemistry-Paraffin: Integrin alpha 6/CD49f Antibody [NBP1-85747] - Staining of human placenta shows strong membranous positivity in trophoblastic cells.
Immunohistochemistry-Paraffin: Integrin alpha 6/CD49f Antibody [NBP1-85747]

Immunohistochemistry-Paraffin: Integrin alpha 6/CD49f Antibody [NBP1-85747]

Immunohistochemistry-Paraffin: Integrin alpha 6/CD49f Antibody [NBP1-85747] - Staining of human rectum shows strong membranous positivity in glandular cells.
Simple Western: Integrin alpha 6/CD49f Antibody [NBP1-85747]

Simple Western: Integrin alpha 6/CD49f Antibody [NBP1-85747]

Simple Western: Integrin alpha 6/CD49f Antibody [NBP1-85747] - Simple Western lane view shows a specific band for ITGA6 in 0.2 mg/ml of RT-4 (left) and U-251MG sp (right) lysate. This experiment was performed under reducing conditions using the 66-440 kDa separation system.
Simple Western: Integrin alpha 6/CD49f Antibody [NBP1-85747]

Simple Western: Integrin alpha 6/CD49f Antibody [NBP1-85747]

Simple Western: Integrin alpha 6/CD49f Antibody [NBP1-85747] - Electropherogram image(s) of corresponding Simple Western lane view. Integrin alpha 6/CD49f antibody was used at 1:50 dilution on RT-4 and U-251MG sp lysates(s).
Integrin alpha 6/CD49f Antibody - BSA Free Western Blot: Integrin alpha 6/CD49f Antibody - BSA Free [NBP1-85747]

Western Blot: Integrin alpha 6/CD49f Antibody - BSA Free [NBP1-85747]

Analysis in human cell lines A-431 and MCF-7 using Anti-ITGA6 antibody. Corresponding ITGA6 RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.

Applications for Integrin alpha 6/CD49f Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Simple Western

1:50

Western Blot

0.04-0.4 ug/ml
Application Notes

IHC-Paraffin, HIER pH 6 retrieval is recommended.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in RT-4 and U-251MG sp, separated by Size, antibody dilution of 1:50, apparent MW was 168 kDa. Separated by Size-Wes, Sally Sue/Peggy Sue.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Integrin alpha 6/CD49f

The ITGA6 protein product is the integrin alpha chain alpha 6. Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. For example, alpha 6 may combine with beta 4 in the integrin referred to as TSP180, or with beta 1 in the integrin VLA-6. Integrins are known to participate in cell adhesion as well as cell-surface mediated signalling. Two transcript variants encoding different isoforms have been found for this gene. (provided by RefSeq)

Alternate Names

CD49f, ITGA6, Platelet gpl, VLA-6

Entrez Gene IDs

3655 (Human)

Gene Symbol

ITGA6

UniProt

P23229

Additional Integrin alpha 6/CD49f Products

Product Documents for Integrin alpha 6/CD49f Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Integrin alpha 6/CD49f Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Integrin alpha 6/CD49f Antibody - BSA Free

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Protocols

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