Integrin alpha E/CD103 Antibody (Ber-ACT8) - BSA Free

Name: Integrin alpha E/CD103 Antibody (Ber-ACT8) - BSA Free
Brand: Novus Biologicals
SKU: NBP1-97564
Price: 179 USD
Availability: InStock

Novus Biologicals | Catalog # NBP1-97564

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Citations (2)

Product Documents

Clone Ber-ACT8 was used by HLDA to establish CD designation.

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Product Specifications
Scientific Data
Applications
Flow Cytometry
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human, Primate

Applications

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Flow (Cell Surface), Immunoprecipitation, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ kappa Clone # Ber-ACT8

Format

BSA Free

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Product Specifications

Immunogen

HTLV-1 induced human T cell line MAPS16

Specificity

The mouse monoclonal antibody Ber-ACT8 recognizes an extracellular epitope of CD103 (alpha E integrin), a type I transmembrane glycoprotein primarily found on intestinal intraepithelial lymphocytes. HLDA V; WS Code A067

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁ kappa

Description

Novus Biologicals Mouse Integrin alpha E/CD103 Antibody (Ber-ACT8) - BSA Free (NBP1-97564) is a monoclonal antibody validated for use in IHC, WB, Flow and IP. Anti-Integrin alpha E/CD103 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Integrin alpha E/CD103 Antibody (Ber-ACT8) - BSA Free

Flow Cytometry: Integrin alpha E/CD103 Antibody (Ber-ACT8) - BSA Free [NBP1-97564]

Flow Cytometry: Integrin alpha E/CD103 Antibody (Ber-ACT8) - BSA Free [NBP1-97564] - Separation of human CD103 positive cells (red-filled) from CD103 negative cells (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood using anti-human CD103 (Ber-ACT8) purified antibody (concentration in sample 3 ug/ml, GAM APC).

Flow Cytometry: Integrin alpha E/CD103 Antibody (Ber-ACT8) - BSA Free [NBP1-97564]

Flow Cytometry: Integrin alpha E/CD103 Antibody (Ber-ACT8) [NBP1-97564] - Staining of CD103 on human peripheral blood cells with anti-CD103 (Ber-ACT8) APC.

Flow Cytometry: Integrin alpha E/CD103 Antibody (Ber-ACT8) - BSA Free [NBP1-97564]

Flow Cytometry: Integrin alpha E/CD103 Antibody (Ber-ACT8) [NBP1-97564] - Staining of CD103 on PHA-activated PBMC with anti-CD103 (Ber-ACT8) FITC.

Flow Cytometry: Integrin alpha E/CD103 Antibody (Ber-ACT8) - BSA Free [NBP1-97564]

Flow Cytometry: Integrin alpha E/CD103 Antibody (Ber-ACT8) [NBP1-97564] - Staining of CD103 on PHA-activated PBMC with anti-CD103 (Ber-ACT8) PE.

Flow Cytometry: Integrin alpha E/CD103 Antibody (Ber-ACT8) - BSA Free [NBP1-97564]

Flow Cytometry: Integrin alpha E/CD103 Antibody (Ber-ACT8) - BSA Free [NBP1-97564] - Staining pattern of human PHA stimulated peripheral blood mononuclear cells stained using anti-human CD103 (Ber-ACT8) purified antibody (concentration in sample 3 ug/ml, GAM APC).

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Applications for Integrin alpha E/CD103 Antibody (Ber-ACT8) - BSA Free

Application

Recommended Usage

Flow Cytometry

1-4 ug/ml

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:200

Immunoprecipitation

1:50

Western Blot

1:1000

Application Notes

This antibody is Cytof ready.

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Advanced Features

Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
Spillover Popups - Visualize the spectra of individual fluorochromes
Antigen Density Selector - Match fluorochrome brightness with antigen density

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Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

Phosphate buffered saline (PBS), pH 7.4

Format

BSA Free

Preservative

15mM Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Integrin alpha E/CD103

CD103 / Integrin alphaE is an integrin subunit that is expressed on intraepithelial lymphocytes, epithelial dendritic cells, lamina propria-derived dendritic cells, a subpopulation of lamina propria T cells, a small subset of peripheral lymphocytes, namely T reg cells, and on activated and TGF-beta stimulated lymphocytes. CD103 is in mature form cleaved into two disulfide-linked chains (C-terminal 150 kDa chain and N-terminal 25 kDa chain). It heterodimerizes with integrin beta7 subunit to form alphaE/beta7 integrin (mucosal lymphocyte 1 antigen), which through binding E-cadherin mediates homing of lymphocytes to the intestinal epithelium, and, in addition to the role in adhesion, may serve as an accessory molecule for intraepithelial lymphocyte activation

Alternate Names

CD103, HUMINAE, ITGAE

Gene Symbol

ITGAE

Additional Integrin alpha E/CD103 Products

Product Documents for Integrin alpha E/CD103 Antibody (Ber-ACT8) - BSA Free

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Certificate of Analysis

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Product Specific Notices for Integrin alpha E/CD103 Antibody (Ber-ACT8) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.