IRF3 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-92028

Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all IRF3 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: VNKSRTRFRIPWKHGLRQDAQQEDFGIFQAWAEATGAYVPGRDKPDLPTWKRNFRSALNRKEGLRLAEDRSKDPHDPHKIYEFVNSGVGDFSQPDTSPDTNGGGSTSDTQEDILDELLGNMVLAPLPDPGPPSLAVAPEPCPQ

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit IRF3 Antibody - BSA Free (NBP1-92028) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for IRF3 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: IRF3 Antibody [NBP1-92028]

Immunocytochemistry/ Immunofluorescence: IRF3 Antibody [NBP1-92028]

Immunocytochemistry/Immunofluorescence: IRF3 Antibody [NBP1-92028] - Immunofluorescent staining of human cell line A-431 shows localization to cytosol.
IRF3 Antibody

Immunohistochemistry-Paraffin: IRF3 Antibody [NBP1-92028] -

Immunohistochemistry-Paraffin: IRF3 Antibody [NBP1-92028] -Staining of human small intestine shows moderate cytoplasmic positivity in glandular cells.
IRF3 Antibody - BSA Free Immunohistochemistry: IRF3 Antibody - BSA Free [NBP1-92028]

Immunohistochemistry: IRF3 Antibody - BSA Free [NBP1-92028]

Staining of human lymph node shows weak cytoplasmic positivity in germinal center cells.
IRF3 Antibody - BSA Free Western Blot: IRF3 Antibody - BSA Free [NBP1-92028]

Western Blot: IRF3 Antibody - BSA Free [NBP1-92028]

Analysis in A-549 cells transfected with control siRNA, target specific siRNA probe #1. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
IRF3 Antibody - BSA Free Immunohistochemistry: IRF3 Antibody - BSA Free [NBP1-92028]

Immunohistochemistry: IRF3 Antibody - BSA Free [NBP1-92028]

Staining of human small intestine shows moderate cytoplasmic positivity in glandular cells.
IRF3 Antibody - BSA Free Immunohistochemistry: IRF3 Antibody - BSA Free [NBP1-92028]

Immunohistochemistry: IRF3 Antibody - BSA Free [NBP1-92028]

Staining of human placenta shows moderate cytoplasmic positivity in trophoblastic cells
IRF3 Antibody - BSA Free Immunohistochemistry: IRF3 Antibody - BSA Free [NBP1-92028]

Immunohistochemistry: IRF3 Antibody - BSA Free [NBP1-92028]

Staining of human lung shows moderate cytoplasmic positivity in macrophages.

Applications for IRF3 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200-1:500

Western Blot

0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF,Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IRF3

IRF3 is a family of interferon (IFN) regulatory factors (IRFs) have been shown to play a role in transcription of IFN genes as well as IFN-stimulated genes. The IRF-3 gene encodes a 50-kDa protein that binds specifically to the IFN-stimulated response element (ISRE) but not to the IRF-1 binding site PRD-I. Overexpression of IRF-3 stimulates expression of the IFN-stimulated gene 15 (ISG15) promoter, an ISRE-containing promoter. he high amino acid homology between IRF-3 and ISG factor 3 gamma polypeptide (ISGF3 gamma) and their similar binding properties indicate that, like ISGF3 gamma, IRF-3 may activate transcription by complex formation with other transcriptional factors, possibly members of the Stat family (1). IRF-3 is expressed constitutively in a variety of tissues, and the relative levels of IRF-3 mRNA do not change in virus-infected or IFN-treated cells. (2). In uninfected cells, the IRF-3 component of DRAF1 resides in the cytoplasm. The cytoplasmic localization of IRF-3 is dependent on a nuclear export signal, and IRF-3 is recognized by the chromosome region maintenance 1 (CRM1) (also known as exportin 1) shuttling receptor. Following infection and specific phosphorylation, IRF-3 accumulates in the nucleus where it associates with CBP and p300 (3).

Long Name

Interferon Regulatory Factor 3

Alternate Names

interferon regulatory factor 3, IRF-3

Gene Symbol

IRF3

Additional IRF3 Products

Product Documents for IRF3 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for IRF3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for IRF3 Antibody - BSA Free

There are currently no reviews for this product. Be the first to review IRF3 Antibody - BSA Free and earn rewards!

Have you used IRF3 Antibody - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies