![Western Blot: IRF5 Antibody (10T1)BSA Free [NBP1-04307]](https://resources.rndsystems.com/images/products/IRF5-Antibody-10T1-Western-Blot-NBP1-04307-img0005.jpg "Western Blot: IRF5 Antibody (10T1)BSA Free [NBP1-04307]")
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, Flow (Intracellular), Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 10T1
Format
BSA Free
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Product Specifications
Immunogen
Recombinant human IRF5 (176-240 aa) purified from E. coli
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Scientific Data Images for IRF5 Antibody (10T1) - BSA Free
Western Blot: IRF5 Antibody (10T1)BSA Free [NBP1-04307]
Western Blot: IRF5 Antibody (10T1) [NBP1-04307] - The extracts of Ramos, THP-1, A20 and NIH3T3 were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human IRF5 antibody (1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.![Immunocytochemistry/ Immunofluorescence: IRF5 Antibody (10T1) - BSA Free [NBP1-04307]](https://resources.rndsystems.com/images/products/IRF5-Antibody-10T1-Immunocytochemistry-Immunofluorescence-NBP1-04307-img0008.jpg "Immunocytochemistry/ Immunofluorescence: IRF5 Antibody (10T1) - BSA Free [NBP1-04307]")
Immunocytochemistry/ Immunofluorescence: IRF5 Antibody (10T1) - BSA Free [NBP1-04307]
Immunocytochemistry/Immunofluorescence: IRF5 Antibody (10T1) [NBP1-04307] - Analysis in THP-1 cells line, stained with DAPI (Blue) for nucleus staining and antibody at 1:100 with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).![Immunohistochemistry-Paraffin: IRF5 Antibody (10T1) - BSA Free [NBP1-04307]](https://resources.rndsystems.com/images/products/IRF5-Antibody-10T1-Immunohistochemistry-Paraffin-NBP1-04307-img0003.jpg "Immunohistochemistry-Paraffin: IRF5 Antibody (10T1) - BSA Free [NBP1-04307]")
Immunohistochemistry-Paraffin: IRF5 Antibody (10T1) - BSA Free [NBP1-04307]
Immunohistochemistry-Paraffin: IRF5 Antibody (10T1) [NBP1-04307] - Human IRF-5 antibody (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB).![Flow (Intracellular): IRF5 Antibody (10T1) - BSA Free [NBP1-04307]](https://resources.rndsystems.com/images/products/IRF5-Antibody-10T1-Flow-Intracellular-NBP1-04307-img0010.jpg "Flow (Intracellular): IRF5 Antibody (10T1) - BSA Free [NBP1-04307]")
Flow (Intracellular): IRF5 Antibody (10T1) - BSA Free [NBP1-04307]
Flow (Intracellular): IRF5 Antibody (10T1) [NBP1-04307] - An intracellular stain was performed on THP-1 cells with IRF5 Antibody (10T1) NBP1-04307PE (blue) and a matched isotype control(orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.![Flow Cytometry: IRF5 Antibody (10T1) - BSA Free [NBP1-04307]](https://resources.rndsystems.com/images/products/IRF5-Antibody-10T1-Flow-Cytometry-NBP1-04307-img0009.jpg "Flow Cytometry: IRF5 Antibody (10T1) - BSA Free [NBP1-04307]")
Flow Cytometry: IRF5 Antibody (10T1) - BSA Free [NBP1-04307]
Flow Cytometry: IRF5 Antibody (10T1) [NBP1-04307] - Flow cytometry analysis of IRF5 in THP-1 cell line, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).Applications for IRF5 Antibody (10T1) - BSA Free
Application
Recommended Usage
ELISA
1:100-1:2000
Flow Cytometry
2-5ug
Immunocytochemistry/ Immunofluorescence
1:10-1:500
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Paraffin
1:50
Western Blot
1:500-1:1000
Reviewed Applications
Read 1 review rated 5 using NBP1-04307 in the following applications:
Flow Cytometry Panel Builder
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Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.
Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH 7.4), 10% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: IRF5
Long Name
Interferon Regulatory Factor 5
Alternate Names
IBD14, interferon regulatory factor 5, IRF-5, SLEB10
Gene Symbol
IRF5
UniProt
Additional IRF5 Products
Product Documents for IRF5 Antibody (10T1) - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for IRF5 Antibody (10T1) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Customer Reviews for IRF5 Antibody (10T1) - BSA Free (1)
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Application: Western BlotSample Tested: Dendritic cellsSpecies: HumanVerified Customer | Posted 08/30/2021IRF5 Antibody, dendritic cells.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Liperfluo
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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