IRF6 Antibody

Immunohistochemistry-Paraffin: IRF6 Antibody [NBP1-51911]

Immunohistochemistry-Paraffin: IRF6 Antibody [NBP1-51911] - staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Western Blot: IRF6 Antibody [NBP1-51911] -

PPAR gamma Trans-Represses IRF6 in Cerebrovascular Endothelial Cells. (A) Diagram of the pGL 4.10 luciferase reporter plasmid carrying the murine IRF6 gene promoter sequence 5′-upstream of Luc. Two putative PPRE binding sites were identified at −2206/−2190 bp (PPRE site 1) and −11626/−11606 bp (PPRE site 2). Transcription starts at +1 bp. (B) A luciferase reporter assay was conducted by transfecting murine cerebrovascular endothelial cells with the pGL 4.10 luciferase reporter plasmid delivering either the IRF6 wild-type promoter sequence (IRF6 PPRE WT) or the IRF6 promoter sequence carrying a mutation at one of the two PPRE sites (IRF6 PPRE site 1 mutant or IRF6 PPRE site 2 mutant). Pioglitazone and/or PPAR gamma gain-of-function significantly elevated IRF6 wild-type promoter activity but did not affect IRF6 PPRE site 1 mutant promoter activity. *P < 0.05 versus control group, †P < 0.05 versus Pioglitazone group, ‡P < 0.05 versus Ad.PPAR gamma group. (C,D) Real-time PCR on the total RNA content from control and small-hairpin PPAR gamma RNA (PPAR gamma shRNA)-transfected murine cerebrovascular endothelial cells revealed that PPAR gamma silencing significantly elevates IRF6 mRNA expression, particularly more significantly under oxygen-glucose deprivation (OGD) conditions. Cropped blots are displayed here. *P < 0.05 versus same-condition Control group, †P < 0.05 versus Non-OGD PPAR gamma shRNA group. Data are reported as means +/- standard errors of the mean (SEMs). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/28526834), licensed under a CC-BY license. Not internally tested by Novus Biologicals.