![Western Blot: IRF7 AntibodyBSA Free [NBP3-06987]](https://resources.rndsystems.com/images/products/IRF7-Antibody-Western-Blot-NBP3-06987-img0005.jpg "Western Blot: IRF7 AntibodyBSA Free [NBP3-06987]")
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Knockout Validated, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
Antibody was raised against a peptide corresponding to 17 amino acids near the center of human IRF7. The immunogen is located within amino acids 200-230 of IRF7.
Specificity
Several isoforms of IRF7 are known to exist. Human IRF7 has 4 isoforms, including isoform A (503aa, 54kD), isoform B (474aa, 52kD), isoform C (164aa, 18kD) and isoform D (516aa, 56kD). Mouse IRF7 has one isoform (457aa, 51kD) and Rat IRF7 also has one isoform (456aa, 51kD). NBP3-06987 can detect human isoforms and mouse, and possibly can detect rat.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for IRF7 Antibody - BSA Free
Western Blot: IRF7 AntibodyBSA Free [NBP3-06987]
Western Blot: IRF7 Antibody [NBP3-06987] - Loading: 15 ug of lysates per lane. Antibodies: NBP3-06987(1 ug/mL), 1 hr incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution![Western Blot: IRF7 AntibodyBSA Free [NBP3-06987]](https://resources.rndsystems.com/images/products/IRF7-Antibody-Western-Blot-NBP3-06987-img0001.jpg "Western Blot: IRF7 AntibodyBSA Free [NBP3-06987]")
Western Blot: IRF7 AntibodyBSA Free [NBP3-06987]
Western Blot: IRF7 Antibody [NBP3-06987] - Loading: 10ug of A549 WT cell lysates or IRF7 KO cell lysates. Antibodies: NBP3-06987 (0.5 ug/mL) and beta-actin, 1 hr incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.![Western Blot: IRF7 AntibodyBSA Free [NBP3-06987]](https://resources.rndsystems.com/images/products/IRF7-Antibody-Western-Blot-NBP3-06987-img0002.jpg "Western Blot: IRF7 AntibodyBSA Free [NBP3-06987]")
Western Blot: IRF7 AntibodyBSA Free [NBP3-06987]
Western Blot: IRF7 Antibody [NBP3-06987] - Loading: 15 ug of lysates per lane. Antibodies: NBP3-06987(1 ug/mL), 1 hr incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution![Western Blot: IRF7 AntibodyBSA Free [NBP3-06987]](https://resources.rndsystems.com/images/products/IRF7-Antibody-Western-Blot-NBP3-06987-img0003.jpg "Western Blot: IRF7 AntibodyBSA Free [NBP3-06987]")
Western Blot: IRF7 AntibodyBSA Free [NBP3-06987]
Western Blot: IRF7 Antibody [NBP3-06987] - Loading: 15 ug of lysates per lane. Antibodies: NBP3-06987(1 ug/mL), 1 hr incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution![Western Blot: IRF7 AntibodyBSA Free [NBP3-06987]](https://resources.rndsystems.com/images/products/IRF7-Antibody-Western-Blot-NBP3-06987-img0004.jpg "Western Blot: IRF7 AntibodyBSA Free [NBP3-06987]")
Western Blot: IRF7 AntibodyBSA Free [NBP3-06987]
Western Blot: IRF7 Antibody [NBP3-06987] - Loading: 15 ug of lysates per lane. Antibodies: NBP3-06987(1 ug/mL), 1 hr incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution
Immunohistochemistry: IRF7 Antibody - BSA Free [NBP3-06987] -
Immunohistochemistry: IRF7 Antibody - BSA Free [NBP3-06987] - Validation of IRF7 in Mouse Pancreas Tissue. Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti-IRF7 antibody at 2 u/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Immunohistochemistry: IRF7 Antibody - BSA Free [NBP3-06987] -
Immunohistochemistry: IRF7 Antibody - BSA Free [NBP3-06987] - Validation of IRF7 in Human Liver Tissue.Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-IRF7 antibody at 2 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Immunohistochemistry: IRF7 Antibody - BSA Free [NBP3-06987] -
Immunohistochemistry: IRF7 Antibody - BSA Free [NBP3-06987] - Validation of IRF7 in Rat Brain Tissue.Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-IRF7 antibody at 5 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Immunohistochemistry: IRF7 Antibody - BSA Free [NBP3-06987] -
Immunohistochemistry: IRF7 Antibody - BSA Free [NBP3-06987] - Validation of IRF7 in Human Colon Tissue.Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-IRF7 antibody at 2 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.![Immunohistochemistry: IRF7 Antibody - BSA Free [NBP3-06987]](https://resources.rndsystems.com/images/products/IRF7-Antibody-Immunohistochemistry-NBP3-06987-img0008.jpg "Immunohistochemistry: IRF7 Antibody - BSA Free [NBP3-06987]")
Immunohistochemistry: IRF7 Antibody - BSA Free [NBP3-06987]
Immunohistochemistry: IRF7 Antibody [NBP3-06987] - Immunohistochemical analysis of paraffin-embedded human pancreas tissue using NBP3-06987 at 2 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 degrees C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Immunohistochemistry: IRF7 Antibody - BSA Free [NBP3-06987] -
Immunohistochemistry: IRF7 Antibody - BSA Free [NBP3-06987] - Validation of IRF7 in Rat Brain Tissue.Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-IRF7 antibody at 5 u/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Immunocytochemistry/ Immunofluorescence: IRF7 Antibody - BSA Free [NBP3-06987] -
Immunocytochemistry/ Immunofluorescence: IRF7 Antibody - BSA Free [NBP3-06987] - Validation of IRF7 in Mouse Raw264.7 Cells.Immunofluorescent analysis of 4% paraformaldehyde-fixed Raw264.7 cells labeling IRF7 at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).Applications for IRF7 Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
20 ug/ml
Immunohistochemistry
2-5 ug/mL
Immunohistochemistry-Paraffin
2-5 ug/mL
Western Blot
0.5-1 ug/mL
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: IRF7
Alternate Names
interferon regulatory factor 7, interferon regulatory factor-7H, IRF-7, IRF7A, IRF-7H
Gene Symbol
IRF7
Additional IRF7 Products
Product Documents for IRF7 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for IRF7 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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