Irisin/FNDC5 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Irisin/FNDC5 Antibody - BSA Free [NBP2-59680] -

Irisin decreases the oxidative stress, calcium influx and ER stress after gut IR. Irisin (250 μg/kg in 0.5 mL saline, a single dose, iv) was administered immediately after reperfusion. Anti‐irisin (4 mg/kg, Abcam, USA) blocking antibody was administered at 24 h before gut IR. Four hours after reperfusion, mice were sacrificed, and tissue samples were collected. A,B, DHE fluorescence staining of gut tissues; (C‐G) levels of gut malonaldehyde (MDA), xanthine oxidase (XO), 4‐hydroxynonenal (4‐HNT), superoxide dismutase (SOD) and glutathione peroxidase activity (GSH‐PX), respectively; n = 6 per group, mean +/- SEM, *P <.05 vs the sham group, #P <.05 vs the gut IR group. Caco‐2 cells were exposed to hypoxia for 90 min, and 10 nmol/L irisin was added at the beginning of reoxygenation. (H,I) DHE fluorescence staining; (J,K) Fluo‐4 AM staining of Ca2+; (L,M) Western blot analysis of IRE1 and CHOP expression at 4 h after reoxygenation in Caco‐2 cells; n = 3 per group, mean +/- SEM, *P <.05 vs the sham group, #P <.05 vs the H/R group Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/31701659), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: Irisin/FNDC5 Antibody - BSA Free [NBP2-59680] -

Irisin protects against gut IR injury via binding to integrin alpha v beta 5 receptor in enterocyte. Caco‐2 cells were exposed to hypoxia for 90 min, and 10 nmol/L irisin and 20 μmol/L cilengitide trifluoroacetate were added at the beginning of reoxygenation. A, Immunofluorescence staining of irisin (green), integrin alpha V beta 5 (red) and the corresponding nuclear counterstaining (blue) in Caco‐2 cells at 4 h after reoxygenation in Caco‐2 cells. Irisin (250 μg/kg, iv) and cilengitide trifluoroacetate (20 mg/kg, iv) were administered immediately after reperfusion. Four hours after reperfusion, mice were sacrificed, and tissue samples were collected. (B) CO‐IP of irisin and integrin alpha V beta 5; (C) serum FITC‐dextran levels; (D) colony‐forming units (CFUs) from mesenteric lymph node (MLN) tissues; (E) haematoxylin and eosin (H&E) staining; (F) gut injury score; n = 6 per group, mean +/- SEM, *P <.05 vs the gut IR group, #P <.05 vs the gut IR + irisin group. (G,H) Western blot analysis of claudin‐1 and occludin expression in Caco‐2 cells; and (I,J) immunofluorescence staining of JAM‐1 and ZO‐1 and the corresponding nuclear counterstaining (blue). * in the figure represents intercellular gap. n = 3 per group, mean +/- SEM, *P <.05 vs the sham group, #P <.05 vs the H/R group Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/31701659), licensed under a CC-BY license. Not internally tested by Novus Biologicals.