Irisin, also known as FNDC5, is a peptide hormone that is upregulated in skeletal muscle and subcutaneous adipose tissue during exercise. It induces expression of the mitochondrial proteins PPAR-gamma coactivator 1 alpha (PGC1a) and uncoupling protein-1 (UCP1). It contributes to the regulation of diabetes and obesity by inducing the transition of white adipose tissue into more metabolically active beige adipose tissue. Irisin also regulates neuronal cell differentiation, neurite outgrowth, and osteoblast differentiation.
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Recombinant Human, Rat, Porcine, Bat, Bovine, Canine, Equine, Feline, Guinea Pig, Goat, Ferret, Mammal, Mink, Monkey Irisin/FNDC5 Protein
Novus Biologicals | Catalog # NBP3-57463
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Key Product Details
Applications
Western Blot, ELISA, Cell Culture
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Product Specifications
Description
A recombinant protein corresponding to 112 aa of Bat,Bovine,Canine,Equine,Feline,Ferret,Goat,Guinea Pig,Human,Mammal,Mink,Monkey,Porcine,Rat Irisin/FNDC5.
Source: Yeast
Amino Acid Sequence: DSPSAPVNVT VRHLKANSAV VSWDVLEDEV VIGFAISQQK KDVRMLRFIQ EVNTTTRSCA LWDLEEDTEY IVHVQAISIQ GQSPASEPVL FKTPREAEKM ASKNKDEVTM KE (112)
Stable for up to 12 months from date of receipt at -20C. Stable for 3 months when stored in working aliquots with a carrier protein at -20C. Avoid repeated freeze/thaw cycles.
Purity
>98%, by SDS-PAGE
Endotoxin Level
No affinity tags & naturally endotoxin-free
Predicted Molecular Mass
12.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Applications
Enzyme-linked Immunosorbent Spot (ELISPOT)
ELISA Standard (Matched Pair) (ELISA(Sta))
ELISA Standard (Matched Pair) (ELISA(Sta))
Protein / Peptide Type
Recombinant Protein
Formulation, Preparation, and Storage
NBP3-57463
| Formulation | Lyophilized in 1-5X PBS containing 10% Trehalose |
| Preservative | 10% Trehalose |
| Concentration | Lyoph |
| Reconstitution | Reconstitute in sterile PBS with at least 0.1% carrier protein |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Store at -20C. Avoid freeze-thaw cycles. |
Calculators
Background: Irisin/FNDC5
Long Name
Fibronectin Type III Domain Containing 5
Alternate Names
FNDC5, FRCP2
Gene Symbol
FNDC5
Additional Irisin/FNDC5 Products
Product Documents
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices
This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- A Guide to the Colony Forming Cell Assay: Methods and Tips
- Cellular Response to Hypoxia Protocols
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- Definitive Endoderm Differentiation of BG01V Human Embryonic Stem Cells
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- General Support Buffers
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
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- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Protocol for CEPT Cocktail Kit
- Protocol for Cell Counting Kit-8
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- Protocol for Culturing Rat Microglia
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- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
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- R&D Systems Quality Control Western Blot Protocol
- Rat Cortical Stem Cell Culture Expansion Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- The Human Colony Forming Cell (CFC) Assay using Methylcellulose-based Media
- The Mouse Colony Forming Cell (CFC) Assay using Methylcellulose-based Media
- The Mouse Pre-B Colony Forming Cell (CFC) Assay Using Methylcellulose-based Media
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Western Blot Figures
- Use of Cultrex Basement Membrane Extract to Improve Take and Growth of Xenografts in Mice
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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