ISG15/UCRP Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-16995

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human, Rat

Applications

Knockout Validated, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all ISG15/UCRP Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human ISG15/UCRP. The exact sequence is proprietary.

Localization

Cytoplasm; Secreted

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

18 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ISG15/UCRP Antibody - BSA Free

Knockout Validated: ISG15/UCRP Antibody [NBP2-16995]

Western Blot: ISG15/UCRP Antibody [NBP2-16995]

Western Blot: ISG15/UCRP Antibody [NBP2-16995] - Wild-type (WT) and ISG15 knockout (KO) HeLa cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with ISG15 antibody. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Immunocytochemistry/ Immunofluorescence: ISG15/UCRP Antibody [NBP2-16995]

Immunocytochemistry/ Immunofluorescence: ISG15/UCRP Antibody [NBP2-16995]

Immunocytochemistry/Immunofluorescence: ISG15/UCRP Antibody [NBP2-16995] - HepG2 cells were fixed in 4% paraformaldehyde for 10 min. Green: ISG15 protein stained by ISG15 antibody diluted at 1:100. Blue: Hoechst 33342 staining.Scale bar = 10 um.
Immunohistochemistry-Paraffin: ISG15/UCRP Antibody [NBP2-16995]

Immunohistochemistry-Paraffin: ISG15/UCRP Antibody [NBP2-16995]

Immunohistochemistry-Paraffin: ISG15/UCRP Antibody [NBP2-16995] - Human gastric cancer, using ISG15 antibody antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
ISG15/UCRP Antibody

Western Blot: ISG15/UCRP Antibody [NBP2-16995] -

Western Blot: ISG15/UCRP Antibody [NBP2-16995] - Untreated (-) and treated (+) A549 whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with ISG15/UCRP antibody (NBP2-16995) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for ISG15/UCRP Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Format

BSA Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: ISG15/UCRP

ISG15 is a ubiquitin-like protein that becomes conjugated to many cellular proteins upon activation by interferon-alpha (IFNA; MIM 147660) and -beta (IFNB; MIM 147640) (Zhao et al., 2005 [PubMed 16009940]).[supplied by OMIM]

Long Name

ISG15 Ubiquitin-like Modifier

Alternate Names

G1P2, IFI15, IP17, UCRP

Gene Symbol

ISG15

Additional ISG15/UCRP Products

Product Documents for ISG15/UCRP Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for ISG15/UCRP Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

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