ITCH/AIP4 Antibody

Novus Biologicals | Catalog # NB100-68142

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Canine (100%), Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Western Blot, Peptide ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all ITCH/AIP4 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-EIKSHDLKPNGGN corresponding to internal region according to NP_113671.3.

Epitope

EIKSHDLKPNGGN

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for ITCH/AIP4 Antibody

Western Blot: ITCH/AIP4 Antibody [NB100-68142]

Western Blot: ITCH/AIP4 Antibody [NB100-68142]

Western Blot: ITCH/AIP4 Antibody [NB100-68142] - Staining of Human Brain (Frontal Cortex) lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Flow Cytometry: ITCH/AIP4 Antibody [NB100-68142]

Flow Cytometry: ITCH/AIP4 Antibody [NB100-68142]

Flow Cytometry: ITCH/AIP4 Antibody [NB100-68142] - Analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Immunofluorescence: ITCH/AIP4 Antibody [NB100-68142]

Immunofluorescence: ITCH/AIP4 Antibody [NB100-68142]

Immunofluorescence: ITCH/AIP4 Antibody [NB100-68142] - Analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear and vesicle staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Immunofluorescence: ITCH/AIP4 Antibody [NB100-68142]

Immunofluorescence: ITCH/AIP4 Antibody [NB100-68142]

Immunofluorescence: ITCH/AIP4 Antibody [NB100-68142] - Analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear staining. Actin filaments were stained with phalloidin (red). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Applications for ITCH/AIP4 Antibody

Application
Recommended Usage

Flow Cytometry

10 ug/ml

Peptide ELISA

Detection limit 1:16000

Western Blot

0.1 - 0.3 ug/ml
Application Notes
WB: Approx 90kDa band observed in Human Brain (Frontal Cortex) lysates (calculated MW of 98.7kDa according to NP_113671.3).

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ITCH/AIP4

Atrophin-1 contains a polyglutamine repeat, expansion of which is responsible for dentatorubral and pallidoluysian atrophy. The protein encoded by this gene interacts with atrophin-1. This encoded protein is a closely related member of the NEDD4-like protein family. This family of proteins are E3 ubiquitin-ligase molecules and regulate key trafficking decisions, including targeting of proteins to proteosomes or lysosomes. This encoded protein contains four tandem WW domains and a HECT (homologous to the E6-associated protein carboxyl terminus) domain. It can act as a transcriptional corepressor of p45/NFE2 and may participate in the regulation of immune responses by modifying Notch-mediated signaling. It is highly similar to the mouse Itch protein, which has been implicated in the regulation and differentiation of erythroid and lymphoid cells.

Long Name

Itchy E3 Ubiquitin Protein Ligase

Alternate Names

AIF4, AIP4, NAPP1

Entrez Gene IDs

83737 (Human); 16396 (Mouse); 311567 (Rat)

Gene Symbol

ITCH

UniProt

NP_113671.3 (Human)

Additional ITCH/AIP4 Products

Product Documents for ITCH/AIP4 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for ITCH/AIP4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

E3 Ubiquitin Ligases
Notch Pathway

Citations for ITCH/AIP4 Antibody

Customer Reviews for ITCH/AIP4 Antibody

There are currently no reviews for this product. Be the first to review ITCH/AIP4 Antibody and earn rewards!

Have you used ITCH/AIP4 Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies