ITK Antibody (5G6)

Novus Biologicals | Catalog # NBP2-37578

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 5G6
View all ITK Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human ITK expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

72 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ITK Antibody (5G6)

Western Blot: ITK Antibody (5G6)BSA Free [NBP2-37578]

Western Blot: ITK Antibody (5G6)BSA Free [NBP2-37578]

Western Blot: ITK Antibody (5G6) [NBP2-37578] - Analysis using ITK mouse mAb against Jurkat cell lysate.
Immunocytochemistry/ Immunofluorescence: ITK Antibody (5G6) - BSA Free [NBP2-37578]

Immunocytochemistry/ Immunofluorescence: ITK Antibody (5G6) - BSA Free [NBP2-37578]

Immunocytochemistry/Immunofluorescence: ITK Antibody (5G6) [NBP2-37578] - Immunofluorescence analysis of Jurkat cells using ITK mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
Flow Cytometry: ITK Antibody (5G6) - BSA Free [NBP2-37578]

Flow Cytometry: ITK Antibody (5G6) - BSA Free [NBP2-37578]

Flow Cytometry: ITK Antibody (5G6) [NBP2-37578] - Analysis of Jurkat cells using ITK mouse mAb (blue) and negative control (red).
Western Blot: ITK Antibody (5G6)BSA Free [NBP2-37578]

Western Blot: ITK Antibody (5G6)BSA Free [NBP2-37578]

Western Blot: ITK Antibody (5G6) [NBP2-37578] - Analysis using ITK mAb against human ITK (AA: 2-120) recombinant protein. (Expected MW is 39.7 kDa)
ELISA: ITK Antibody (5G6) - BSA Free [NBP2-37578]

ELISA: ITK Antibody (5G6) - BSA Free [NBP2-37578]

ELISA: ITK Antibody (5G6) [NBP2-37578] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Applications for ITK Antibody (5G6)

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200 - 1:400

Immunocytochemistry/ Immunofluorescence

1:200 - 1:1000

Western Blot

1:500 - 1:2000

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Ascites

Formulation

Ascites

Preservative

0.03% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ITK

Emtk/Itk/Tsk is a member of the Tec family of Protein Tyrosine Kinase (PTK) expressed in T, NK, and mast cells (1). Structurally, Emtk/Itk/Tsk possesses a tyrosine kinase (TK), a pleckstrin homology (PH) and a TEC homology domain. It also contains both SRC homology 2 (SH2) and a SRC homology 3 (SH3) domains (2). It lacks as well the amino-terminal myristylation signal and the negative autoregulatory phosphorylation site present in all members of the Src family of PTKs (3). Additionally, the Src tyrosine kinase LCK is required for Emtk/Itk/Tsk activation and recruitment by CD28 (4).

Long Name

IL-2-inducible T-cell Kinase

Alternate Names

EMT, LYK, PSCTK2, Tcsk

Gene Symbol

ITK

Additional ITK Products

Product Documents for ITK Antibody (5G6)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for ITK Antibody (5G6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Natural Killer T (NKT) Cells

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Protocols

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FAQs

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