ITK Antibody

Novus Biologicals | Catalog # NBP1-31732

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Bovine (94%), Mouse (90%), Rat (91%), Rhesus Macaque (98%). Backed by our 100% Guarantee.

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all ITK Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human ITK. The exact sequence is proprietary.

Localization

Cell membrane

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

72 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ITK Antibody

Immunocytochemistry/ Immunofluorescence: ITK Antibody [NBP1-31732]

Immunocytochemistry/ Immunofluorescence: ITK Antibody [NBP1-31732]

Immunocytochemistry/Immunofluorescence: ITK Antibody [NBP1-31732] - Jurkat cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: ITK stained by ITK antibody [N2C1], Internal diluted at 1:500.Blue: Fluoroshield with DAPI.
ITK Antibody

Western Blot: ITK Antibody [NBP1-31732] -

Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ITK antibody [N2C1], Internal (NBP1-31732) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
ITK Antibody

Western Blot: ITK Antibody [NBP1-31732] -

Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ITK antibody [N2C1], Internal (NBP1-31732) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Applications for ITK Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: ITK

IL2-inducible T-cell kinase. This gene encodes an intracellular tyrosine kinase expressed in T-cells. The protein contains both SH2 and SH3 domains which are often found in intracellular kinases. It is thought to play a role in T-cell proliferation and differentiation.

Long Name

IL-2-inducible T-cell Kinase

Alternate Names

EMT, LYK, PSCTK2, Tcsk

Gene Symbol

ITK

Additional ITK Products

Product Documents for ITK Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for ITK Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Related Research Areas

Natural Killer T (NKT) Cells

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

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