ITM2B Antibody

Novus Biologicals | Catalog # NBP1-36963

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Peptide ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all ITM2B Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-HDKETYKLQRRETIK corresponding to internal region according to NP_068839.1.

Epitope

internal region

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for ITM2B Antibody

Western Blot: ITM2B Antibody [NBP1-36963]

Western Blot: ITM2B Antibody [NBP1-36963]

Western Blot: ITM2B Antibody [NBP1-36963] - HepG2 cell lysate (35 ug protein in RIPA buffer). Antibody at 0.3 ug/mL. Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: ITM2B Antibody [NBP1-36963]

Immunocytochemistry/ Immunofluorescence: ITM2B Antibody [NBP1-36963]

Immunocytochemistry/Immunofluorescence: ITM2B Antibody [NBP1-36963] - Paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary antibody at 10 ug/mL, 1 hr incubation followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing Golgi apparatus and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10u g/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).
Immunohistochemistry-Paraffin: ITM2B Antibody [NBP1-36963]

Immunohistochemistry-Paraffin: ITM2B Antibody [NBP1-36963]

Immunohistochemistry-Paraffin: ITM2B Antibody [NBP1-36963] - Human small intestine tissue. Antibody at 2.5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Flow Cytometry: ITM2B Antibody [NBP1-36963]

Flow Cytometry: ITM2B Antibody [NBP1-36963]

Flow Cytometry: ITM2B Antibody [NBP1-36963] - Paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary antibody at 10 ug/mL, 1 hr incubation followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Immunocytochemistry/ Immunofluorescence: ITM2B Antibody [NBP1-36963]

Immunocytochemistry/ Immunofluorescence: ITM2B Antibody [NBP1-36963]

Immunocytochemistry/Immunofluorescence: ITM2B Antibody [NBP1-36963] - Paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary antibody at 10 ug/mL, 1 hr incubation followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing Golgi apparatus and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).

Applications for ITM2B Antibody

Application
Recommended Usage

Flow Cytometry

10 ug/mL

Immunocytochemistry/ Immunofluorescence

10 ug/mL

Immunohistochemistry

2.5 ug/mL

Immunohistochemistry-Paraffin

2.5 ug/mL

Peptide ELISA

Detection limit 1:8000

Western Blot

0.1 - 0.3 ug/mL
Application Notes
Western blot: Approx. 30-35 kDa band observed in HepG2 cell lysates (calculated MW of 30.3 kDa according to NP_068839.1).

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ITM2B

ITM2B is a type II membrane protein. It is expressed in brain and in other tissues. Defects in ITM2B seem to be associated with familial British dementia (FBD) and familial Danish dementia (FDD).

Long Name

Integral Membrane Protein 2B

Alternate Names

ABRI, BRI, BRI2, BRICD2B, E25B, E3-16, FBD

Entrez Gene IDs

9445 (Human); 16432 (Mouse); 290364 (Rat)

Gene Symbol

ITM2B

UniProt

NP_068839.1 (Human)

Additional ITM2B Products

Product Documents for ITM2B Antibody

Certificate of Analysis

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Product Specific Notices for ITM2B Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Aspartic Proteases and Regulators

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Protocols

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FAQs

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