JNK2 Antibody (011) - Azide and BSA Free

Name: JNK2 Antibody (011) - Azide and BSA Free
Brand: Novus Biologicals
SKU: NBP2-89621
Price: 449 USD
Availability: InStock

Novus Biologicals | Catalog # NBP2-89621

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Product Documents

Recombinant Monoclonal Antibody

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 011 expressed in HEK293

Format

Azide and BSA Free

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Product Specifications

Immunogen

This antibody was obtained from a rabbit immunized with purified, recombinant Human JNK2 (Accession#: NP_002743.3; Met 1-Arg 424).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for JNK2 Antibody (011) - Azide and BSA Free

Western Blot: JNK2 Antibody (011) [NBP2-89621]

Western Blot: JNK2 Antibody (011) [NBP2-89621] - Lane A: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane.Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:48 kDa Observed band size:54 kDa (We are unsure as to the identity of these extra bands.)

Immunocytochemistry/ Immunofluorescence: JNK2 Antibody (011) [NBP2-89621]

Immunocytochemistry/Immunofluorescence: JNK2 Antibody (011) [NBP2-89621] - Staining of Human MAPK9 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Rabbit anti-Human MAPK9 monoclonal antibody (1:300) at 37c 1 hour. Then cells were stained with the Alexa Fluor(R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm and Nucleus.

Immunohistochemistry-Paraffin: JNK2 Antibody (011) [NBP2-89621]

Immunohistochemistry-Paraffin: JNK2 Antibody (011) [NBP2-89621] - Staining of human MAPK9 in human brain with rabbit mnoclonal antibody (1:1000).

Immunohistochemistry-Paraffin: JNK2 Antibody (011) [NBP2-89621]

Immunohistochemistry-Paraffin: JNK2 Antibody (011) [NBP2-89621] - Staining of human MAPK9 in human kidney with rabbit monoclonal antibody (1:1000).

Immunoprecipitation: JNK2 Antibody (011) [NBP2-89621]

Immunoprecipitation: JNK2 Antibody (011) [NBP2-89621] - Lane A:0.5 mg HepG2 Whole Cell Lysate Lane B:0.5 mg Hela Whole Cell LysateLane C:0.5 mg Jurkat Whole Cell Lysate Lane D:0.5 mg MCF-7 Whole Cell Lysate0.5 uL anti-MAPK9 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-MAPK9 rabbit monoclonal antibody,at 1:500 dilution Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 44 kDa Observed band size: 44 kDa

Immunocytochemistry/Immunofluorescence: JNK2 Antibody (011) [NBP2-89621] -

Immunocytochemistry/Immunofluorescence: JNK2 Antibody (011) [NBP2-89621] - Staining of Human MAPK9 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-89621 antibody (1:300) at 37c 1 hour. Then cells were stained with the Alexa Fluor(R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm and Nucleus.

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Applications for JNK2 Antibody (011) - Azide and BSA Free

Application

Recommended Usage

ELISA

1:25000-1:50000

Immunocytochemistry/ Immunofluorescence

1:100-1:500

Immunohistochemistry-Paraffin

1:500-1:2500

Immunoprecipitation

1-2 uL/mg of lysate

Western Blot

1:500-1:1000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: JNK2

JNK protein kinases are distantly related to mitogen-activated protein kinases (ERKs) and are activated by dual phosphorylation on Tyr and Thr. The JNK protein kinase group includes the 46-kDa isoform JNK1 and the 55-kDa protein kinase JNK2. The activities of both JNK isoforms are markedly increased by exposure of cells to UV radiation (1). JNK becomes activated in vivo in response to pro-inflammatory cytokines or cellular stresses. Its full activation requires the phosphorylation of a threonine and a tyrosine residue in a Thr-Pro-Tyr motif, which can be catalysed by the protein kinases mitogen-activated protein kinase kinase MKK4 and MKK7 (2). JNK2 functions in a cell-type-specific and stimulus-dependent manner, being required for apoptosis of immature thymocytes induced by anti-CD3 antibody but not for apoptosis induced by anti-Fas antibody, UVC or dexamethasone. JNK2 is not required for activation-induced cell death of mature T cells (3).

Long Name

C-Jun N-terminal Kinase 2

Alternate Names

MAPK9, SAPK1 alpha

Gene Symbol

MAPK9

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Product Specific Notices for JNK2 Antibody (011) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.