KAP1 Antibody (20C1)

Novus Biologicals | Catalog # NB100-74549

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Chromatin Immunoprecipitation (ChIP), Gel Super Shift Assays

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 20C1
View all KAP1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein corresponding to residues 60-383 of the human TIF1 beta protein.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 22110054). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Specificity

TIF1 beta (20C1)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for KAP1 Antibody (20C1)

Western Blot: KAP1 Antibody (20C1) [NB100-74549]

Western Blot: KAP1 Antibody (20C1) [NB100-74549]

Western Blot: KAP1 Antibody (20C1) [NB100-74549] - Analysis of TIF1 beta in gamma irradiated HeLA cells.
Immunocytochemistry/ Immunofluorescence: KAP1 Antibody (20C1) [NB100-74549]

Immunocytochemistry/ Immunofluorescence: KAP1 Antibody (20C1) [NB100-74549]

Immunocytochemistry/Immunofluorescence: KAP1 Antibody (20C1) [NB100-74549] - Gamma irradiated HeLa cells.

Applications for KAP1 Antibody (20C1)

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

1:10-1:500

Gel Super Shift Assays

1:1 - 1:100

Immunocytochemistry/ Immunofluorescence

2 - 4 ug/ml

Immunohistochemistry-Paraffin

1:10-1:500

Immunoprecipitation

1:10 - 1:500

Western Blot

1 ug/ml

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS with 1 mg/ml BSA

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: KAP1

TIF1 beta is a member of the tripartite motif (TRIM) family. It is composed of a N-terminal RBCC (RING finger, B boxes, coiled coil) motif, and a C-terminal bromodomain which is preceded by a PHD finger. TIF1 beta has been reported to function as a co-repressor for Kruppel-associated box (KRAB)-domain-containing zinc finger proteins. As such, it plays a crucial role in early embryogenesis. It appears that the co-repressor function of TIF1 beta is related to its organization and/or maintenance of higher-order, heterochromatin-like structures.

Long Name

KRAB-associated Protein 1

Alternate Names

KRIP-1, RNF96, TF1B, TIF1B, TRIM28

Entrez Gene IDs

10155 (Human); 21849 (Mouse)

Gene Symbol

TRIM28

Additional KAP1 Products

Product Documents for KAP1 Antibody (20C1)

Certificate of Analysis

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Product Specific Notices for KAP1 Antibody (20C1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for KAP1 Antibody (20C1)

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Protocols

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