KAP1 Antibody (HL1539) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-25540

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (93%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL1539 expressed in HEK293

Format

Azide and BSA Free
View all KAP1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human KAP1. The exact sequence is proprietary.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for KAP1 Antibody (HL1539) - Azide and BSA Free

KAP1 Antibody (HL1539) - Azide and BSA Free

Immunohistochemistry-Paraffin: KAP1 Antibody (HL1539) - Azide and BSA Free [NBP3-25540] -

Immunohistochemistry-Paraffin: KAP1 Antibody (HL1539) - Azide and BSA Free [NBP3-25540] - KAP1 antibody [HL1539] detects KAP1 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. KAP1 stained by KAP1 antibody [HL1539] (NBP3-25540) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
KAP1 Antibody (HL1539) - Azide and BSA Free

Western Blot: KAP1 Antibody (HL1539) - Azide and BSA Free [NBP3-25540] -

Western Blot: KAP1 Antibody (HL1539) - Azide and BSA Free [NBP3-25540] - Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with KAP1 antibody [HL1539] (NBP3-25540) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
KAP1 Antibody (HL1539) - Azide and BSA Free

Immunohistochemistry-Paraffin: KAP1 Antibody (HL1539) - Azide and BSA Free [NBP3-25540] -

Immunohistochemistry-Paraffin: KAP1 Antibody (HL1539) - Azide and BSA Free [NBP3-25540] - KAP1 antibody [HL1539] detects KAP1 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded rat colon. KAP1 stained by KAP1 antibody [HL1539] (NBP3-25540) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
KAP1 Antibody (HL1539) - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: KAP1 Antibody (HL1539) - Azide and BSA Free [NBP3-25540] -

Immunocytochemistry/Immunofluorescence: KAP1 Antibody (HL1539) - Azide and BSA Free [NBP3-25540] - KAP1 antibody [HL1539] detects KAP1 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: KAP1 stained by KAP1 antibody [HL1539] (NBP3-25540) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.
KAP1 Antibody (HL1539) - Azide and BSA Free

Western Blot: KAP1 Antibody (HL1539) - Azide and BSA Free [NBP3-25540] -

Western Blot: KAP1 Antibody (HL1539) - Azide and BSA Free [NBP3-25540] - Human tissue extract (30 ug) was separatedby 7.5% SDS-PAGE, and the membrane was blotted with KAP1 antibody [HL1539] (NBP3-25540) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
KAP1 Antibody (HL1539) - Azide and BSA Free

Immunohistochemistry-Paraffin: KAP1 Antibody (HL1539) - Azide and BSA Free [NBP3-25540] -

Immunohistochemistry-Paraffin: KAP1 Antibody (HL1539) - Azide and BSA Free [NBP3-25540] - KAP1 antibody [HL1539] detects KAP1 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. KAP1 stained by KAP1 antibody [HL1539] (NBP3-25540) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
KAP1 Antibody (HL1539) - Azide and BSA Free

Immunohistochemistry-Paraffin: KAP1 Antibody (HL1539) - Azide and BSA Free [NBP3-25540] -

Immunohistochemistry-Paraffin: KAP1 Antibody (HL1539) - Azide and BSA Free [NBP3-25540] - KAP1 antibody [HL1539] detects KAP1 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. KAP1 stained by KAP1 antibody [HL1539] (NBP3-25540) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
KAP1 Antibody (HL1539) - Azide and BSA Free

Western Blot: KAP1 Antibody (HL1539) - Azide and BSA Free [NBP3-25540] -

Western Blot: KAP1 Antibody (HL1539) - Azide and BSA Free [NBP3-25540] - Rat tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with KAP1 antibody [HL1539] (NBP3-25540) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for KAP1 Antibody (HL1539) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:1000-1:10000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: KAP1

KAP1 is encoded by this gene mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. The protein localizes to the nucleus and is thought to associate with specific chromatin regions. The protein is a member of the tripartite motif family. This tripartite motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region.

Long Name

KRAB-associated Protein 1

Alternate Names

KRIP-1, RNF96, TF1B, TIF1B, TRIM28

Gene Symbol

TRIM28

Additional KAP1 Products

Product Documents for KAP1 Antibody (HL1539) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for KAP1 Antibody (HL1539) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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