Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Predicted:

Rat (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all KAP1 Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 650 and 700 of human Tripartite Motif-Containing 28 using the numbering given in entry NP_005753.1 (GeneID 10155).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit KAP1 Antibody - BSA Free (NB500-159) is a polyclonal antibody validated for use in IHC, WB and IP. Anti-KAP1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for KAP1 Antibody - BSA Free

Western Blot: KAP1 Antibody [NB500-159]

Western Blot: KAP1 Antibody [NB500-159]

Western Blot: KAP1 Antibody [NB500-159] - Detection of Human and Mouse KAP-1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-KAP-1 antibody NB500-159 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.
Immunohistochemistry-Paraffin: KAP1 Antibody [NB500-159]

Immunohistochemistry-Paraffin: KAP1 Antibody [NB500-159]

Immunohistochemistry-Paraffin: KAP1 Antibody [NB500-159] - Sample: FFPE section of mouse plasmacytoma Antibody: Affinity purified rabbit anti-Kap-1 Cat. No. Lot4 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).
Immunohistochemistry-Paraffin: KAP1 Antibody [NB500-159]

Immunohistochemistry-Paraffin: KAP1 Antibody [NB500-159]

Immunohistochemistry-Paraffin: KAP1 Antibody [NB500-159] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-Kap-1 Cat. No. Lot4 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).
Immunoprecipitation: KAP1 Antibody [NB500-159]

Immunoprecipitation: KAP1 Antibody [NB500-159]

Immunoprecipitation: KAP1 Antibody [NB500-159] - Detection of human KAP-1 by western blot of immunoprecipitates. Samples Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-KAP-1 antibody NB500-159 (lot NB500-159-4) used for IP at 6 ug per reaction. KAP-1 was also immunoprecipitated by a previous lot of this antibody (lot NB500-159-3) and rabbit anti-KAP-1 antibody NB500-158. For blotting immunoprecipitated KAP-1, NB500-159 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.

Applications for KAP1 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:1000 to 1:5000

Immunohistochemistry-Paraffin

1:1000 to 1:5000

Immunoprecipitation

2-10 ug/mg lysate

Western Blot

1:2000 - 1:10000
Application Notes
By Western Blot, this antibody recognizes a band at ~106 kDa, representing KAP-1. The investigator should determine the optimal working dilution for a specific application. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: KAP1

The nuclear corepressor, KAP-1, is involved in silencing heterochromatin. It has also been suggested to play a role in signal transduction.

Long Name

KRAB-associated Protein 1

Alternate Names

KRIP-1, RNF96, TF1B, TIF1B, TRIM28

Entrez Gene IDs

10155 (Human)

Gene Symbol

TRIM28

UniProt

NP_005753.1 (Human)

Additional KAP1 Products

Product Documents for KAP1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for KAP1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for KAP1 Antibody - BSA Free

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Protocols

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FAQs

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