Kappa Light Chain Antibody (HP6053 + L1C1)

Novus Biologicals | Catalog # NBP2-34258

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Kappa/IgG1 Kappa Clone # HP6053 + L1C1
View all Kappa Light Chain Primary Antibodies »

Product Specifications

Immunogen

Purified human Ig kappa chain (HP6053) & Human B-Lymphoma Cells (L1C1)

Localization

Cell Surface, cytoplasmic and secreted

Marker

B-Cell Marker

Specificity

This monoclonal antibody is specific to kappa light chain of immunoglobulin and shows no cross-reaction with lambda light chain or any of the five heavy chains. In mammals, the two light chains in an antibody are always identical, with only one type of light chain, kappa or lambda. The ratio of Kappa to Lambda is 70:30. However, with the occurrence of multiple myeloma or other B-cell malignancies this ratio is disturbed. Antibody to the kappa light chain is reportedly useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkins lymphomas. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is malignant.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 Kappa/IgG1 Kappa

Theoretical MW

22.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-34640)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Kappa Light Chain Antibody (HP6053 + L1C1)

Western Blot: Kappa Light Chain Antibody (HP6053 + L1C1) [NBP2-34258]

Western Blot: Kappa Light Chain Antibody (HP6053 + L1C1) [NBP2-34258]

Western Blot: Kappa Light Chain Antibody (HP6053 + L1C1) [NBP2-34258] - Analysis of Raji Cell Lysate using kappa Light Chain Monclonal Antibody (HP6053+ L1C1)
Immunocytochemistry/ Immunofluorescence: Kappa Light Chain Antibody (HP6053 + L1C1) [NBP2-34258]

Immunocytochemistry/ Immunofluorescence: Kappa Light Chain Antibody (HP6053 + L1C1) [NBP2-34258]

Immunocytochemistry/Immunofluorescence: Kappa Light Chain Antibody (HP6053 + L1C1) [NBP2-34258] - LP-1 (multiple myeloma) and H929 (multiple myeloma). Image from verified customer review.
Kappa Light Chain Antibody (HP6053 + L1C1)

Immunohistochemistry-Paraffin: Kappa Light Chain Antibody (HP6053 + L1C1) [NBP2-34258] -

Immunohistochemistry-Paraffin: Kappa Light Chain Antibody (HP6053 + L1C1) [NBP2-34258] - Formalin-fixed, paraffin-embedded human Tonsil stained with Kappa Light Chain Antibody (HP6053 + L1C1).

Applications for Kappa Light Chain Antibody (HP6053 + L1C1)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Use in ICC/IF reported by a customer review.

Reviewed Applications

Read 1 review rated 5 using NBP2-34258 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Kappa Light Chain

Kappa light chain antibody can be used for the identification of leukemias, plasmacytomas and certain non-Hodgkin's lymphomas. Kappa light chain contains one immunoglobulin-like domain. The EU sequence has the INV allotypic marker, Ala-45 and Val-83. The ROY sequence has the INV allotypic marker, Ala-45 and Leu-83.

Alternate Names

HCAK1, IGKCD, immunoglobulin kappa constant, Km, MGC111575, MGC62011, MGC72072, MGC88770, MGC88771, MGC88809

Gene Symbol

IGKC

UniProt

P01601 (Human), P01834 (Human)

Additional Kappa Light Chain Products

Product Documents for Kappa Light Chain Antibody (HP6053 + L1C1)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Kappa Light Chain Antibody (HP6053 + L1C1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for Kappa Light Chain Antibody (HP6053 + L1C1) (1)

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  • Kappa Light Chain Antibody (HP6053 + L1C1)
    Name: Natalie Erdmann
    Application: Immunocytochemistry
    Sample Tested: LP-1 (multiple myeloma) and H929 (multiple myeloma)
    Species: Human
    Verified Customer | Posted 07/17/2019
    Immunocytochemistry staining LP-1 and H929 with NBP2-34258
    1. Cells immersion-fixed in 2%PFA 10 minutes, wash PBS X 3 2. Permeabilized 0.5% Triton X-100 5 minutes; wash PBS X 3 3. Block 10% donkey serum 1 hour 4. 1/200 NBP2-34258 O/N @4oC; wash PBS X 3 5. 1/1000 donkey F(ab’)2 Alexa Fluor 647 anti-mouse IgG (H+L) 1 hour @room temp (Jackson #715-606-150); wash PBS X 3 6. Olympus/BIOVIEW Cy5 filter 600ms exposure
    Kappa Light Chain Antibody (HP6053 + L1C1) NBP2-34258

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