Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Kappa Light Chain Secondary Antibodies »

Product Specifications

Immunogen

Human Kappa Light Chain

Specificity

By immunoelectrophoresis and ELISA this reacts specifically with kappa light chains common to all human immunoglobulins. No was detected against lambda light chain or non-immunoglobulin serum proteins.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Goat anti-Human Kappa Light Chain Secondary Antibody

Immunocytochemistry/ Immunofluorescence: Goat anti-Human Kappa Light Chain Secondary Antibody [NB7463]

Immunocytochemistry/ Immunofluorescence: Goat anti-Human Kappa Light Chain Secondary Antibody [NB7463]

Immunocytochemistry/Immunofluorescence: Goat anti-Human Kappa Light Chain Secondary Antibody [NB7463] - JJN3 and 8226 Human myeloma cells stained with NB7463. Cells fixed in 4%PFA 10min, wash PBS X 3. Permeabilized 0.5% Triton X-100 5min; wash PBS X 3. Block 10% donkey serum 1hr. 1:10,000 NB7463 O/N at 4C; wash PBS X 3 and 1:2000 donkey F(ab')2 Alexa Fluor Cy3 anti-goat IgG (H+L) 1 hour at room temp; wash PBS X 3. Olympus/BIOVIEW Cy3 filter 300ms exposure. Image from verified customer review.

Applications for Goat anti-Human Kappa Light Chain Secondary Antibody

Application
Recommended Usage

ELISA

1:1000 - 1:30000

Immunocytochemistry/ Immunofluorescence

1:200- 1:2000

Immunohistochemistry

1:200- 1:2000

Immunohistochemistry-Paraffin

1:200- 1:2000

Western Blot

1:1000 - 1:30000
Application Notes
ELISA 1:1,000 - 1:30,000; for coating plates 1:100 - 1:500

Reviewed Applications

Read 1 review rated 5 using NB7463 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Phosphate Buffered Saline (PBS)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Kappa Light Chain

Kappa light chain antibody can be used for the identification of leukemias, plasmacytomas and certain non-Hodgkin's lymphomas. Kappa light chain contains one immunoglobulin-like domain. The EU sequence has the INV allotypic marker, Ala-45 and Val-83. The ROY sequence has the INV allotypic marker, Ala-45 and Leu-83.

Alternate Names

HCAK1, IGKCD, immunoglobulin kappa constant, Km, MGC111575, MGC62011, MGC72072, MGC88770, MGC88771, MGC88809

Gene Symbol

IGKC

Additional Kappa Light Chain Products

Product Documents for Goat anti-Human Kappa Light Chain Secondary Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Goat anti-Human Kappa Light Chain Secondary Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Goat anti-Human Kappa Light Chain Secondary Antibody

Customer Reviews for Goat anti-Human Kappa Light Chain Secondary Antibody (1)

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  • Goat anti-Human Kappa Light Chain Secondary Antibody
    Name: Natalie Erdmann
    Application: Immunocytochemistry
    Sample Tested: JJN3
    Species: Human
    Verified Customer | Posted 04/08/2019
    Multiple myeloma cells JJN3 and 8226 stained with NB7463
    1. Cells fixed in 4%PFA 10 minutes, wash PBS X 3 2. Permeabilized 0.5% Triton X-100 5 minutes; wash PBS X 3 3. Block 10% donkey serum 1 hour 4. 1/10,000 NB7463 O/N @4oC; wash PBS X 3 5. 1/2000 donkey F(ab’)2 Alexa Fluor Cy3 anti-goat IgG (H+L) 1 hour @room temp (Jackson # 705-166-147); wash PBS X 3 6. Olympus/BIOVIEW Cy3 filter 300ms exposure
    Goat anti-Human Kappa Light Chain Secondary Antibody NB7463

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Protocols

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FAQs

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