KCC2/SLC12A5 Antibody (S1-12) - BSA Free

Western Blot: KCC2/SLC12A5 Antibody (S1-12) - BSA Free [NBP2-59337] -

VU0500469 potentiates KCC2 by a unique mechanism. All experiments conducted using the Cl− flux assay in HEK-293 cells overexpressing KCC2 at 37C, unless otherwise noted. (A) Schematic depicting investigated small molecule targets known to regulate Cl− homeostasis. (B) Co-treatment of modulators of Cl− homeostasis from (A) with or without VU0500469 (n ≥ 16). (C) Left: representative Western blot of biotinylated surface fraction from cells treated with vehicle (DMSO), VU0916219 (VU219), or the positive control phorbol 12-myristate 13-acetate (PMA). Band intensity was normalized to Transferrin Receptor (TfR) levels. O, oligomeric KCC2, M, monomeric KCC2. Right: quantification of Surface KCC2/Total KCC2 normalized to TfR levels. (D) Left: representative Western blot of total protein fraction from cells treated as in (C). Right: quantification of total KCC2 normalized to TfR levels. (n = 4) Error bars represent SD. Statistical significance calculated by Mann–Whitney U test (B) or paired t-test (C,D): * = p < 0.05, *** = p < 0.001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35813195), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: KCC2/SLC12A5 Antibody (S1-12) - BSA Free [NBP2-59337] -

VU0500469 potentiates KCC2 by a unique mechanism. All experiments conducted using the Cl− flux assay in HEK-293 cells overexpressing KCC2 at 37C, unless otherwise noted. (A) Schematic depicting investigated small molecule targets known to regulate Cl− homeostasis. (B) Co-treatment of modulators of Cl− homeostasis from (A) with or without VU0500469 (n ≥ 16). (C) Left: representative Western blot of biotinylated surface fraction from cells treated with vehicle (DMSO), VU0916219 (VU219), or the positive control phorbol 12-myristate 13-acetate (PMA). Band intensity was normalized to Transferrin Receptor (TfR) levels. O, oligomeric KCC2, M, monomeric KCC2. Right: quantification of Surface KCC2/Total KCC2 normalized to TfR levels. (D) Left: representative Western blot of total protein fraction from cells treated as in (C). Right: quantification of total KCC2 normalized to TfR levels. (n = 4) Error bars represent SD. Statistical significance calculated by Mann–Whitney U test (B) or paired t-test (C,D): * = p < 0.05, *** = p < 0.001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35813195), licensed under a CC-BY license. Not internally tested by Novus Biologicals.