KDEL Antibody (10C3) - BSA Free

Novus Biologicals | Catalog # NBP1-97469

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Avian, Chicken, Invertebrate, Mammal, Plant, Primate, Yeast

Cited:

Human, Mouse, Rat, Avian - Chicken, Plant, Primate, Yeast

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Electron Microscopy

Cited:

Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Immunohistochemistry Free-Floating, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, IF/IHC, Westen Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 10C3

Format

BSA Free
View all KDEL Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide corresponding to aa 649-654 (S649EKDEL654) of rat Grp78.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions. Chicken reactivity reported in (PMID: 12163544). Primate reactivity reported in (PMID:12368856). Mouse reactivity reported in (PMID: 18547789). Rat reactivity reported in (PMID: 12969271).

Marker

Endoplasmic Reticulum Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

94 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for KDEL Antibody (10C3) - BSA Free

Western Blot: KDEL Antibody (10C3) [NBP1-97469]

Western Blot: KDEL Antibody (10C3) [NBP1-97469]

Western Blot: KDEL Antibody (10C3) [NBP1-97469] - Lane 1: Grp78 (BiP) recombinant protein, Lane 2: RK-13, Lane 3: Mouse liver microsomes, Lane 4: Rat liver microsomes, Lane 5: HeLa Cell Lysate (heat shocked).
Immunocytochemistry/ Immunofluorescence: KDEL Antibody (10C3) [NBP1-97469]

Immunocytochemistry/ Immunofluorescence: KDEL Antibody (10C3) [NBP1-97469]

Immunocytochemistry/Immunofluorescence: KDEL Antibody (10C3) [NBP1-97469] - KDEL in human primary fibroblasts. Antibody at 1:600 in PBST, 0.1% triton + 10% DS. O/N incubation at 4C. Donkey anti-mouse Alexa Fluor 488 at 1:500 in PBST, 0.1% triton + 10% DS 1h RT. ICC/IF image submitted by a verified customer review.
Immunohistochemistry-Paraffin: KDEL Antibody (10C3) [NBP1-97469]

Immunohistochemistry-Paraffin: KDEL Antibody (10C3) [NBP1-97469]

Immunohistochemistry-Paraffin: KDEL Antibody (10C3) [NBP1-97469] - Analysis of human spleen tissue stained with KDEL, mAb (10C3) at 10 ug/mL.
Immunocytochemistry/ Immunofluorescence: KDEL Antibody (10C3) [NBP1-97469]

Immunocytochemistry/ Immunofluorescence: KDEL Antibody (10C3) [NBP1-97469]

Immunocytochemistry/Immunofluorescence: KDEL Antibody (10C3) [NBP1-97469] - Analysis of endoplasmic reticulum staining of mouse C2C12 myoblasts transfected with wild type mouse ADAM12.

Applications for KDEL Antibody (10C3) - BSA Free

Application
Recommended Usage

Electron Microscopy

1:10 - 1:500

Flow Cytometry

1:10 - 1:1000

Immunocytochemistry/ Immunofluorescence

1:10 - 1:500

Immunohistochemistry

1:10 - 1:500

Immunohistochemistry-Paraffin

1:10 - 1:500

Western Blot

1:1000
Application Notes
Use in ICC/IF reported in scientific literature (PMID:34576258).

Reviewed Applications

Read 2 reviews rated 4.5 using NBP1-97469 in the following applications:

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.2), 50% Glycerol

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: KDEL

The tetrapeptide KDEL, located at the carboxy-terminal sequences of luminal proteins, is a retrieval motif essential for the precise sorting of these proteins along the secretory pathway. KDEL proteins perform essential functions in the endoplasmic reticulum (ER) related to protein folding as well as assembly. The localization of chaperones and other soluble proteins to the ER is achieved by their continuous retrieval from post-ER compartments by the KDEL receptor (Erd2p), which is a membrane protein localized in the Golgi apparatus.

Alternate Names

Endoplasmic reticulum resident protein 58, EP58KDEL1, ER protein 58, ERp58, KDEL (Lys-Asp-Glu-Leu) containing 1, KDEL motif-containing 1, KDEL motif-containing protein 1, MGC5302

Gene Symbol

POGLUT2

Additional KDEL Products

Product Documents for KDEL Antibody (10C3) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for KDEL Antibody (10C3) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for KDEL Antibody (10C3) - BSA Free

Customer Reviews for KDEL Antibody (10C3) - BSA Free (2)

4.5 out of 5
2 Customer Ratings
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Customer Images

Showing  1 - 2 of 2 reviews Showing All
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  • KDEL Antibody (10C3)
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Human primary fibroblast
    Species: Human
    Verified Customer | Posted 10/31/2020
    KDEL antibody in human primary fibroblasts. GRP94 and GRP78 bands. Primary antibody 1:1000 incubation O/N 4ºC in Milk 5% in PBST (0.1% Tween).
    Primary antibody 1:1000 incubation O/N 4ºC in Milk 5% in PBST (0.1% Tween)
    KDEL Antibody (10C3) - BSA Free NBP1-97469
  • KDEL Antibody (10C3)
    Name: Anonymous
    Application: Immunocytochemistry
    Sample Tested: Human primary fibroblast
    Species: Human
    Verified Customer | Posted 02/25/2020
    KDEL in human primary fibroblasts. Dilution 1:600;higher dilution needed; in PBST;0.1% triton; + 10% DS. O/N incubation at 4ºC. Donkey anti-mouse Alexa fluor 488 1:500 in PBST ;0.1% triton; + 10% DS 1h RT.
    Great ab for ICC-IF in human fibroblasts. Ab dilution 1:600 (higher dilution needed) in PBST (0.1% triton) + 10% DS. O/N incubation at 4ºC. Secondary Donkey anti-mouse Alexa fluor 488 1:500 in PBST (0.1% triton) + 10% DS 1h RT.
    KDEL Antibody (10C3) - BSA Free NBP1-97469

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