KDM2A/FBXL11 Antibody

Novus Biologicals | Catalog # NB100-74602

Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation, Chromatin Immunoprecipitation (ChIP)

Cited:

Western Blot, Block/Neutralize, Chemotaxis, Knockdown Validated

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all KDM2A/FBXL11 Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 825 and 875 of human JmjC domain-containing histone demethylation protein 1A using the numbering given in entry NP_036440.1 (GeneID 22992).

Localization

Nuclear

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

133 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for KDM2A/FBXL11 Antibody

Western Blot: KDM2A/FBXL11 Antibody [NB100-74602]

Western Blot: KDM2A/FBXL11 Antibody [NB100-74602]

Western Blot: KDM2A/FBXL11 Antibody [NB100-74602] - Detection of Human JHDM1A by Western Blot. Samples: Whole cell lysate from HeLa (50, 15, 5 ug) and 293T (50 ug) cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-JHDM1A antibody NB100-74602 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunohistochemistry-Paraffin: KDM2A/FBXL11 Antibody [NB100-74602]

Immunohistochemistry-Paraffin: KDM2A/FBXL11 Antibody [NB100-74602]

Immunohistochemistry-Paraffin: KDM2A/FBXL11 Antibody [NB100-74602] - Section of human lung cancer. Antibody: Affinity purified rabbit anti- JHDM1A used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Immunohistochemistry-Paraffin: KDM2A/FBXL11 Antibody [NB100-74602]

Immunohistochemistry-Paraffin: KDM2A/FBXL11 Antibody [NB100-74602]

Immunohistochemistry-Paraffin: KDM2A/FBXL11 Antibody [NB100-74602] - Analysis of human lung tumor tissue using anti-KDM2A antibody. Image submitted by a verified customer review.
Immunoprecipitation: KDM2A/FBXL11 Antibody [NB100-74602]

Immunoprecipitation: KDM2A/FBXL11 Antibody [NB100-74602]

Immunoprecipitation: KDM2A/FBXL11 Antibody [NB100-74602] - Detection of human JHDM1A by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-JHDM1A antibody NB100-74602 (lot NB100-74602-2) used for IP at 6 ug per reaction. JHDM1A was also immunoprecipitated by a previous lot of this antibody (lot NB100-74602-1). For blotting immunoprecipitated JHDM1A, NB100-74602 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
KDM2A/FBXL11 Antibody

Knockdown Validated: KDM2A/FBXL11 Antibody [NB100-74602] -

Transcriptional and stability analysis of F508del-CFTR after demethylases downregulation. (A) CFTR mRNA levels determined by quantitative real-time PCR in F508del-CFTR expressing CFBE41o- cells were transfected with two different siRNAs for each target (JMJD6, KDM2A, KDM3B) for 48 h. CFTR mRNA expression was normalized to 18S RNA and reported relative to its expression in control (SCR) cells that was arbitrarily set to 1 (means +/- SD values, n = 5). (B) F508del-CFTR overexpressing CFBE41o- cells were transfected with siRNA targeting JMJD6, KDM2A, KDM3B or with scrambled siRNAs for 48 h. Subsequently, protein synthesis was inhibited by adding 100 ug/mL cycloheximide (CHX) and cells were harvested after 30, 60 and 90 min. Protein lysates were analyzed by western blot with an anti-CFTR antibody. Calnexin was used as a loading control. The lower-right graph represents the densitometric quantification of the immunostained bands of F508del-CFTR band B normalized by the value at time = 0 (mean +/- SD, n = 4; * p < 0.05 vs. the value of the same time-point of SCR). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36077010), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
KDM2A/FBXL11 Antibody

Western Blot: KDM2A/FBXL11 Antibody [NB100-74602] -

Mutational analysis of CFTR methylated lysine and effect of demethylases downregulation on chaperone machinery. (A) CFBE41o- cells were transiently transfected with F508del-CFTR, or other derivative mutants as indicated. After 24 h, cells were treated with 3 μM VX-809 and the amounts of F508del-CFTR band B and band C were assessed by western blotting. alpha -tubulin was used as loading control (upper panel). In the lower panel, the graphs show the ratio of F508del-CFTR band C/band B for the cells treated with VX-809, obtained by the densitometric quantification of the immunostained band C normalized by band B expression, and expressed as a percentage of the control cells (Ctrl) (means +/- SD values, n = 4; * p < 0.05 vs. Ctrl). (B) F508del-CFTR expressing CFBE41o- cells were transfected with siRNA (#1 and #2 guides) targeting KDM2A or KDM3B or with a scrambled (SCR) siRNA for 48 h. In the left and central panels, the expression of the indicated chaperones was analysed by western blotting. alpha -tubulin was used as loading control. In the right panel, the graphs report the densitometric quantification of the immunostained bands of the experiment detailed in the left and central panels normalized by alpha -tubulin expression, and expressed as a percentage of the control cells (SCR). (Means +/- SD values, n = 3; * p < 0.05 vs. SCR). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36077010), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for KDM2A/FBXL11 Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:100-1:500

Immunoprecipitation

2-10 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. Use in chromatin immunoprecipitation reported in scientific literature (PMID: 30132864). KDM2A/FBXL11 antibody validated for IHC-P from a verified customer review.

Reviewed Applications

Read 2 reviews rated 5 using NB100-74602 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: KDM2A/FBXL11

This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbls class and, in addition to an F-box, contains at least 6 highly degenerated leucine-rich repeats.

Long Name

Lysine-specific demethylase 2A

Alternate Names

CXXC8, EC 1.14.11.27, F-box protein FBL7, FBL11, FBL7, FBXL11, JHDM1A, KDM2A, KIAA1004

Entrez Gene IDs

22992 (Human)

Gene Symbol

KDM2A

UniProt

Q9Y2K7 (Human)

Additional KDM2A/FBXL11 Products

Product Documents for KDM2A/FBXL11 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for KDM2A/FBXL11 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for KDM2A/FBXL11 Antibody

Customer Reviews for KDM2A/FBXL11 Antibody (2)

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Customer Images

Showing  1 - 2 of 2 reviews Showing All
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  • Name: Hunain Alam
    Application: Immunocytochemistry
    Sample Tested: Human lung tumor
    Species: Human
    Verified Customer | Posted 10/08/2015
    IHC staining on humna lung cancer tissue section
    KDM2A/FBXL11 Antibody NB100-74602
  • Name: Hunain Alam
    Application: Western Blot
    Sample Tested: Lung cell lines whole cell lysate
    Species: Human
    Verified Customer | Posted 10/08/2015
    Lung cell lines whole cell lysate
    KDM2A/FBXL11 Antibody NB100-74602

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Protocols

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