KDM6A Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-80628

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Predicted:

Rat (94%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Chemotaxis, IF/IHC, Knockdown Validated

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Lysine (K)-specific Demethylase 6A/KDM6A/UTX Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: IGNNHITGSGSNGNVPYLQRNALTLPHNRTNLTSSAEEPWKNQLSNSTQGLHKGQSSHSAGPNGERPLSSTGPSQHLQAAGSGIQNQNGHPTLPSNSVTQGAALNHLSSHTATSGGQQGITLTKESKPSGNILTVPETSRHT

Reactivity Notes

Mouse reactivity reported from a verified customer review.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for KDM6A Antibody - BSA Free

Immunohistochemistry-Paraffin: KDM6A Antibody [NBP1-80628]

Immunohistochemistry-Paraffin: KDM6A Antibody [NBP1-80628]

Immunohistochemistry-Paraffin: KDM6A Antibody [NBP1-80628] - Staining in human bone marrow and skeletal muscle tissues using NBP1-80628 antibody. Corresponding KDM6A RNA-seq data are presented for the same tissues.
Western Blot: KDM6A Antibody [NBP1-80628]

Western Blot: KDM6A Antibody [NBP1-80628]

KDM6A-Antibody-Western-Blot-NBP1-80628-img0022.jpg
Western Blot: KDM6A Antibody [NBP1-80628]

Western Blot: KDM6A Antibody [NBP1-80628]

Western Blot: KDM6A Antibody [NBP1-80628] - Analysis in human cell line HEK 293.
Western Blot: KDM6A Antibody [NBP1-80628]

Western Blot: KDM6A Antibody [NBP1-80628]

Western Blot: KDM6A Antibody [NBP1-80628] - MCF-7 and MDA-MB-231 whole cell lysates (40 ug/lane). 10% SDS-PAGE. KDM6A Antibody (NBP1-80628) primary antibody at 1:1000, 4C, overnight. Western blot image submitted by a verified customer review.
Immunohistochemistry-Paraffin: KDM6A Antibody [NBP1-80628]

Immunohistochemistry-Paraffin: KDM6A Antibody [NBP1-80628]

Immunohistochemistry-Paraffin: KDM6A Antibody [NBP1-80628] - Red: UTX nuclear colocalization with DAPI (in blue). Image submitted by a verified customer review.
Immunohistochemistry-Paraffin: KDM6A Antibody [NBP1-80628]

Immunohistochemistry-Paraffin: KDM6A Antibody [NBP1-80628]

Immunohistochemistry-Paraffin: KDM6A Antibody [NBP1-80628] - Staining of human bone marrow shows high expression.
Immunohistochemistry-Paraffin: KDM6A Antibody [NBP1-80628]

Immunohistochemistry-Paraffin: KDM6A Antibody [NBP1-80628]

Immunohistochemistry-Paraffin: KDM6A Antibody [NBP1-80628] - Staining of human cervix shows moderate to strong nuclear positivity in squamous epithelial cells.
Immunohistochemistry-Paraffin: KDM6A Antibody [NBP1-80628]

Immunohistochemistry-Paraffin: KDM6A Antibody [NBP1-80628]

Immunohistochemistry-Paraffin: KDM6A Antibody [NBP1-80628] - Staining of human Fallopian tube shows moderate nuclear positivity in glandular cells.
Immunohistochemistry-Paraffin: KDM6A Antibody [NBP1-80628]

Immunohistochemistry-Paraffin: KDM6A Antibody [NBP1-80628]

Immunohistochemistry-Paraffin: KDM6A Antibody [NBP1-80628] - Staining of human lymph node shows moderate to strong nuclear positivity in germinal center cells.
Immunohistochemistry-Paraffin: KDM6A Antibody [NBP1-80628]

Immunohistochemistry-Paraffin: KDM6A Antibody [NBP1-80628]

Immunohistochemistry-Paraffin: KDM6A Antibody [NBP1-80628] - Staining of human skeletal muscle shows no positivity in myocytes as expected.

Applications for KDM6A Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04 - 0.4 ug/mL
Application Notes
IHC-Paraffin, HIER pH 6 retrieval is recommended.

Reviewed Applications

Read 2 reviews rated 5 using NBP1-80628 in the following applications:

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Lysine (K)-specific Demethylase 6A/KDM6A/UTX

UTX - ubiquitously transcribed tetratricopeptide repeat, X chromosome

Alternate Names

KABUK2, KDM6A, Lysine (K)specific Demethylase 6A, UTX

Entrez Gene IDs

7403 (Human)

Gene Symbol

KDM6A

Additional Lysine (K)-specific Demethylase 6A/KDM6A/UTX Products

Product Documents for KDM6A Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for KDM6A Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Epigenetics

Citations for KDM6A Antibody - BSA Free

Customer Reviews for KDM6A Antibody - BSA Free (2)

5 out of 5
2 Customer Ratings
5 Stars
100%
4 Stars
0%
3 Stars
0%
2 Stars
0%
1 Stars
0%

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Customer Images

Showing  1 - 2 of 2 reviews Showing All
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  • KDM6A Antibody
    Name: Yongkang Yang
    Application: Western Blot
    Sample Tested: breast cancer cell line, Sample Amount: 40
    Species: Human
    Verified Customer | Posted 02/26/2021
    Western Blot: MCF-7 and MDA-MB-231 whole cell lysates were loaded with 40 ug/lane. 10% SDS-PAGE. KDM6A Antibody (NBP1-80628) primary antibody: 1:1000, 4℃, overnight.  
    KDM6A Antibody - BSA Free NBP1-80628
  • KDM6A Antibody
    Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: mouse uterus
    Species: Mouse
    Verified Customer | Posted 01/19/2017
    Red: UTX nuclear colocalization with DAPI (in blue)
    Mouse uterus fixed in 4% PFA overnight. Citrate buffer retrieval, boiling for 10 minutes.
    KDM6A Antibody - BSA Free NBP1-80628

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Protocols

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FAQs

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