Keap1 Antibody (1F10B6)

Novus Biologicals | Catalog # NBP2-37433

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 1F10B6
View all Keap1 Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human Keap1 (AA: 380-624) expressed in E. Coli.

Reactivity Notes

Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

69.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Keap1 Antibody (1F10B6)

Western Blot: Keap1 Antibody (1F10B6)BSA Free [NBP2-37433]

Western Blot: Keap1 Antibody (1F10B6)BSA Free [NBP2-37433]

Western Blot: Keap1 Antibody (1F10B6) [NBP2-37433] - Analysis using KEAP1 mouse mAb against NIH3T3 (1), and A549 (2) cell lysate.
Immunocytochemistry/ Immunofluorescence: Keap1 Antibody (1F10B6) - BSA Free [NBP2-37433]

Immunocytochemistry/ Immunofluorescence: Keap1 Antibody (1F10B6) - BSA Free [NBP2-37433]

Immunocytochemistry/Immunofluorescence: Keap1 Antibody (1F10B6) [NBP2-37433] - Analysis of HeLa cells using KEAP1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Flow Cytometry: Keap1 Antibody (1F10B6) - BSA Free [NBP2-37433]

Flow Cytometry: Keap1 Antibody (1F10B6) - BSA Free [NBP2-37433]

Flow Cytometry: Keap1 Antibody (1F10B6) [NBP2-37433] - Analysis of HepG2 cells using KEAP1 mouse mAb (green) and negative control (purple).
Western Blot: Keap1 Antibody (1F10B6)BSA Free [NBP2-37433]

Western Blot: Keap1 Antibody (1F10B6)BSA Free [NBP2-37433]

Western Blot: Keap1 Antibody (1F10B6) [NBP2-37433] - Analysis using KEAP1 mAb against human KEAP1 recombinant protein. (Expected MW is 52.7 kDa)
ELISA: Keap1 Antibody (1F10B6) - BSA Free [NBP2-37433]

ELISA: Keap1 Antibody (1F10B6) - BSA Free [NBP2-37433]

ELISA: Keap1 Antibody (1F10B6) [NBP2-37433] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Applications for Keap1 Antibody (1F10B6)

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200 - 1:400

Immunocytochemistry/ Immunofluorescence

1:50

Western Blot

1:500 - 1:2000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Ascites

Formulation

Ascites

Preservative

0.03% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Keap1

The KEAP1 gene encodes a protein containing KELCH-1 like domains, as well as a BTB/POZ domain. Kelch-like ECH-associatedprotein 1 interacts with NF-E2-related factor 2 in a redox-sensitive manner and the dissociation of the proteins inthe cytoplasm is followed

Long Name

Kelch-like ECH-associated Protein 1

Alternate Names

INrf2

Gene Symbol

KEAP1

Additional Keap1 Products

Product Documents for Keap1 Antibody (1F10B6)

Certificate of Analysis

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Product Specific Notices for Keap1 Antibody (1F10B6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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