Ki67/MKI67 Antibody (1297A) - BSA Free

Novus Biologicals | Catalog # NBP2-54791

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Knockout Validated, Immunohistochemistry, Immunohistochemistry-Paraffin, Flow Cytometry, Flow (Intracellular), Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry, Western Blot, Flow Cytometry, IF/IHC

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 1297A expressed in HEK293

Format

BSA Free
View all Ki67/MKI67 Primary Antibodies »

Product Specifications

Immunogen

The immunogen for this KI67/MKI67 Antibody (1297A) was made using a synthetic peptide from the internal portion of Mouse KI67/MKI67, between amino acids 1850-1950 [UniProt# E9PVX6].

Reactivity Notes

Use in Mouse reported in scientific literature (PMID:33536573).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

351 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Ki67/MKI67 Antibody (1297A) - BSA Free

Immunocytochemistry/ Immunofluorescence: Ki67/MKI67 Antibody (1297A) - BSA Free [NBP2-54791]

Immunocytochemistry/ Immunofluorescence: Ki67/MKI67 Antibody (1297A) - BSA Free [NBP2-54791]

Immunocytochemistry/Immunofluorescence: Ki67/MKI67 Antibody (1297A) [NBP2-54791] - A431 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-Ki67/MKI67 Antibody (1297A) NBP2-54791 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:1000 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.
Knockout Validated: Ki67/MKI67 Antibody (1297A) - BSA Free [NBP2-54791]

Knockout Validated: Ki67/MKI67 Antibody (1297A) - BSA Free [NBP2-54791]

Knockout Validated: Ki67/MKI67 Antibody (1297A) [NBP2-54791] - Ki67/MKI67 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line but is not detected in Ki67/MKI67 knockout (KO) HeLa cell line using Rabbit Anti-Human Ki67/MKI67 Monoclonal Antibody (Catalog # NBP2-54791) at 1 ug/mL for 3 hours at room temperature. Cells were stained using the NorthernLights(TM) 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to nuclei.
Immunocytochemistry/ Immunofluorescence: Ki67/MKI67 Antibody (1297A) - BSA Free [NBP2-54791]

Immunocytochemistry/ Immunofluorescence: Ki67/MKI67 Antibody (1297A) - BSA Free [NBP2-54791]

Immunocytochemistry/Immunofluorescence: Ki67/MKI67 Antibody (1297A) [NBP2-54791] - NIH3T3 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti- NBP2-54791 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:1000 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.
Immunocytochemistry/ Immunofluorescence: Ki67/MKI67 Antibody (1297A) - BSA Free [NBP2-54791]

Immunocytochemistry/ Immunofluorescence: Ki67/MKI67 Antibody (1297A) - BSA Free [NBP2-54791]

Immunocytochemistry/Immunofluorescence: Ki67/MKI67 Antibody (1297A) [NBP2-54791] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with Ki67/MKI67 Antibody [1297A] conjugated to Alexa Fluor 647 (NBP2-54791AF647) at 2 ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Immunohistochemistry-Paraffin: Ki67/MKI67 Antibody (1297A) - BSA Free [NBP2-54791]

Immunohistochemistry-Paraffin: Ki67/MKI67 Antibody (1297A) - BSA Free [NBP2-54791]

Immunohistochemistry-Paraffin: Ki67/MKI67 Antibody (1297A) [NBP2-54791] - Ki-67/MKI67 was detected in immersion fixed paraffin-embedded sections of human liver cancer tissue using Rabbit Anti-Human Ki-67/MKI67 Monoclonal Antibody at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody. Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. Staining with VisUCyte HRP Polymer Detection Reagents.
Flow (Intracellular): Ki67/MKI67 Antibody (1297A) - BSA Free [NBP2-54791]

Flow (Intracellular): Ki67/MKI67 Antibody (1297A) - BSA Free [NBP2-54791]

Flow (Intracellular): Ki67/MKI67 Antibody (1297A) [NBP2-54791] - Ki-67/MKI67 Antibody (1297A) [NBP2-54791] - An intracellular stain was performed on Jurkat Cells with Ki-67/MKI67 (1297A) antibody NBP2-54791 (blue) and a matched isotype control MAB1050 (orange). Cells were fixed with 4% paraformaldehyde, following fixation, cells were permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by rabbit IgG APC-conjugated secondary antibody (F0111, R&D Systems).
Immunocytochemistry/ Immunofluorescence: Ki67/MKI67 Antibody (1297A) - BSA Free [NBP2-54791]

Immunocytochemistry/ Immunofluorescence: Ki67/MKI67 Antibody (1297A) - BSA Free [NBP2-54791]

Immunocytochemistry/Immunofluorescence: Ki67/MKI67 Antibody (1297A) [NBP2-54791] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-Ki67/MKI67 (1297A) at 2.0 ug/mL overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Flow Cytometry: Ki67/MKI67 Antibody (1297A) - BSA Free [NBP2-54791]

Flow Cytometry: Ki67/MKI67 Antibody (1297A) - BSA Free [NBP2-54791]

Flow Cytometry: Ki67/MKI67 Antibody (1297A) [NBP2-54791] - An intracellular stain was performed on HeLa cells with Ki67/MKI67 Antibody [1297A] NBP2-54791APC (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Allophycocyanin.
Ki67/MKI67 Antibody (1297A) - BSA Free

Immunohistochemistry-Paraffin: Ki67/MKI67 Antibody (1297A) [NBP2-54791] -

Immunohistochemistry-Paraffin: Ki67/MKI67 Antibody (1297A) [NBP2-54791] - Ki67/MKI67 immunoreactivity in mouse small intestine. NBP2-54791 was diluted to 0.5ug/mL in Antibody Diluent and was left on sections for 1h at room temperature. Secondary was Horse Anti-Rabbit HRP. Image from verified customer review.
Ki67/MKI67 Antibody (1297A) - BSA Free

Ki67/MKI67 (1297A) in A431 Human Cell Line.

Ki67/MKI67 (1297A) was detected in immersion fixed A431 human skin carcinoma cell line using Rabbit anti-Ki67/MKI67 (1297A) Protein G Purified Recombinant Monoclonal Antibody conjugated to Alexa Fluor® 488 (Catalog # NBP2-54791AF488) (green) at 2 µg/mL overnight at 4C. Cells were counterstained with DAPI (blue). Cells were imaged using a 100X objective and digitally deconvolved.
Ki67/MKI67 Antibody (1297A) - BSA Free

Detection of Ki67/MKI67 (1297A) in U-2 OS Human Cell Line by Flow Cytometry.

An intracellular stain was performed on U-2 OS human osteosarcoma cell line with Rabbit anti-Ki67/MKI67 (1297A) Protein-G purified Monoclonal Antibody conjugated to Alexa Fluor® 488 (Catalog # NBP2-54791AF488, blue histogram) or matched control antibody (Catalog # NBP2-24982, orange histogram).
Ki67/MKI67 Antibody (1297A) - BSA Free

Immunohistochemistry-Paraffin: Rabbit Monoclonal Ki67/MKI67 Antibody (1297A) [NBP2-54791]

Immunohistochemistry-Paraffin: Rabbit Monoclonal Ki67/MKI67 Antibody (1297A) [NBP2-54791] - Ki67 immunoreactivity in FFPE sections of human tonsil. NBP2-54791 was used at 500ng per mL and was left on tissue sections for 1hr at room temperature. Image from a verified customer review.
Ki67/MKI67 Antibody (1297A) - BSA Free

Ki67/MKI67 Antibody(1297A) in A431 Human Cell Line.

Ki67/MKI67 (1297A) was detected in immersion fixed A431 human skin carcinoma cell line using Rabbit anti-Ki67/MKI67 (1297A) Protein G Purified Monoclonal Antibody conjugated to FITC (Catalog # NBP2-54791F) (green) at 5 µg/mL overnight at 4C. Cells were counterstained with DAPI (blue). Cells were imaged using a 100X objective and digitally deconvolved.

Applications for Ki67/MKI67 Antibody (1297A) - BSA Free

Application
Recommended Usage

Flow (Intracellular)

1 ug/mL

Flow Cytometry

1 ug/mL

Immunocytochemistry/ Immunofluorescence

1-10 ug/mL

Immunohistochemistry

3-15 ug/mL

Immunohistochemistry-Paraffin

3-15 ug/mL

Reviewed Applications

Read 2 reviews rated 5 using NBP2-54791 in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Ki67/MKI67

Ki67 is a nuclear protein associated with proliferation, named after its discovery in a Hodgkin's lymphoma-derived cell line by Scholzer and Gerdes at Kiel University, Germany (1). This proliferation marker is expressed during active phases of the cell cycle (late G1, S, G2 and M), whereas the protein remains undetectable in the G0 phase. Ki67 has two main isoforms with theoretical molecular weights of 319 kDa and 359 kDa. Its abundance is tightly regulated by synthesis and degradation with an estimated half-life of 60-90 min, regardless of its position in the cell cycle. Ki67 has been shown to be involved in ribosomal biogenesis, heterochromatin maintenance, and mitotic chromosome separation (2).

Detection of Ki67 by immunostaining is commonly used as a proliferation marker in solid tumors, as well as certain hematological malignancies (3-5). The Ki67 index, which reports on positive Ki67 stained tumor cell nuclei, has been extensively studied as a prognostic biomarker in cancers such as breast cancer and cervical cancer.

References

1. Gerdes J, Schwab U, Lemke H, Stein H. (1983) Production of a mouse monoclonal antibody reactive with a human nuclear antigen associated with cell proliferation. Int J Cancer. 31:13-20. PMID: 6339421

2. Starborg M, Gell K, Brundell E and Hoog C. (1996) The murine Ki-67 cell proliferation antigen accumulates in the nucleolar and heterochromatic regions of interphase cells and at the periphery of the mitotic chromosomes in a process essential for cell cycle progression. J Cell Sci. 109:143-153. 1996

3. Karamitopoulou E, Perentes E, Tolnay M, Probst A. (1998) Prognostic significance of MIB-1, p53, and bcl-2 immunoreactivity in meningiomas. Hum Pathol. 29(2):140-5. PMID: 9490273

4. Geyer FC, Rodrigues DN, Weigelt B and Reis-Filho JS. (2012) Molecular classification of estrogen receptor-positive/luminal breast cancers. Adv Anat Pathol. 19(1):39-53. PMID: 22156833

5. Ikenberg H, Bergeron C, Schmidt D, Griesser H, Alameda F, Angeloni C, Bogers J, Dachez R, Denton K, Hariri J, Keller T, von Knebel Doeberitz M, Neumann HH, Puig-Tintore LM, Sideri M, Rehm S, Ridder R; PALMS Study Group. (2013) Screening for cervical cancer precursors with p16/Ki-67 dual-stained cytology: results of the PALMS study. J Natl Cancer Inst. 105(20):1550-7. PMID: 24096620

Long Name

Antigen Identified by Monoclonal Antibody Ki67

Alternate Names

Ki-67, KIA, MIB-1, MKI67, PPP1R105, TSG126, antiKi67, Ki67 flow cytometry, Ki-67 flow cytometry, Ki67 ihc, Ki-67 ihc, Ki67 mouse, Ki-67 mouse, Ki67 western blot, Ki-67 western blot

Gene Symbol

MKI67

Additional Ki67/MKI67 Products

Product Documents for Ki67/MKI67 Antibody (1297A) - BSA Free

Certificate of Analysis

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Product Specific Notices for Ki67/MKI67 Antibody (1297A) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cancer Biomarkers
Cell Cycle

Citations for Ki67/MKI67 Antibody (1297A) - BSA Free

Customer Reviews for Ki67/MKI67 Antibody (1297A) - BSA Free (2)

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Customer Images

Showing  1 - 2 of 2 reviews Showing All
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  • Ki67/MKI67 Antibody (1297A) - BSA Free
    Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Tonsil
    Species: Human
    Verified Customer | Posted 04/28/2025
    Ki67 immunoreactivity in FFPE sections of human tonsil. NBP254791 was used at 500ng per mL and was left on tissue sections for 1hr at room temperature.
    Ki67/MKI67 Antibody (1297A) - BSA Free NBP2-54791
  • Ki67/MKI67 Antibody (1297A) - BSA Free
    Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Small intestine
    Species: Mouse
    Verified Customer | Posted 05/12/2023
    Ki67 immunoreactivity in mouse small intestine. NBP2-54791 was diluted to 0.5ug/mL in Antibody Diluent and was left on sections for 1h at room temperature. Secondary was Horse Anti-Rabbit HRP.
    Requires at least heat-induced epitope retrieval. I used Target Retrieval Solution. Slides were placed in retrieval solution that was preheated to 95 degrees Celcius and left for 20m.
    Ki67/MKI67 Antibody (1297A) - BSA Free NBP2-54791

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Protocols

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FAQs for Ki67/MKI67 Antibody (1297A) - BSA Free

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  • Q: Whats the concentration of this Ki67/MKI67 Antibody?

    A: The concentration is lot specific available upon request.

  • Q: Whats the concentration of this Ki67/MKI67 Antibody?

    A:  Optimal concentrations and conditions for each application should be determined by the user.

  • Q: Whats the concentration of this Ki67/MKI67 Antibody?

    A: The concentration is lot specific available upon request.

  • Q: Whats the concentration of this Ki67/MKI67 Antibody?

    A:  Optimal concentrations and conditions for each application should be determined by the user.

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