Ki67/MKI67 Antibody (1E1G1)

Novus Biologicals | Catalog # NBP3-57756

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Knockout Validated, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 1E1G1
View all Ki67/MKI67 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide. This information is considered to be commercially sensitive. (Swissprot# E9PVX6)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This monoclonal antibody cocktail is highly specific and optimized for use in Immunohistochemistry.

Normal Tissues Tested
Pancreas, Lung, Tonsil, Cervix, Placenta, Colon, Kidney, Spleen, Liver, esophagus

Cancer Tissues Tested
Breast cancer, Colon cancer, Thyroid cancer, Hepatocellular Carcinoma, Hepatocholangio carcinoma, Lung adenocarcinoma, Lung squamous carcinoma

Positive Controls
Preferentially during late G1, S, G2 and M phases of the cell cycle tonsil (A moderate to strong, distinct nuclear staining reaction in 80-90% of the germinal centre B-cells in both the light and the dark zone and in the vast majority of the suprabasal squamous epithelial cells), esophagus (squamous epithelia)

Negative Controls
Liver (Hepatocytes, 1%)

Scientific Data Images for Ki67/MKI67 Antibody (1E1G1)

Ki67/MKI67 Antibody (1E1G1) Immunocytochemistry/ Immunofluorescence: Ki67/MKI67 Antibody (1E1G1) [NBP3-57756]

Immunocytochemistry/ Immunofluorescence: Ki67/MKI67 Antibody (1E1G1) [NBP3-57756]

Confocal imaging of C2C12 cells using NBP3-57756 (dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). The cells were counterstained with alpha-Tubulin Mouse mAb (dilution 1:400) followed by incubation with conjugated Goat Anti-Mouse IgG (H+L) Ab (dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
Ki67/MKI67 Antibody (1E1G1) Immunocytochemistry/ Immunofluorescence: Ki67/MKI67 Antibody (1E1G1) [NBP3-57756]

Immunocytochemistry/ Immunofluorescence: Ki67/MKI67 Antibody (1E1G1) [NBP3-57756]

Confocal imaging of A-431 cells using NBP3-57756 (dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). The cells were counterstained with alpha-Tubulin Mouse mAb (dilution 1:400) followed by incubation with conjugated Goat Anti-Mouse IgG (H+L) Ab (dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
Ki67/MKI67 Antibody (1E1G1) Immunocytochemistry/ Immunofluorescence: Ki67/MKI67 Antibody (1E1G1) [NBP3-57756]

Immunocytochemistry/ Immunofluorescence: Ki67/MKI67 Antibody (1E1G1) [NBP3-57756]

Confocal imaging of HeLa cells using NBP3-57756 (dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). The cells were counterstained with alpha-Tubulin Mouse mAb (dilution 1:400) followed by incubation with conjugated Goat Anti-Mouse IgG (H+L) Ab (dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
Ki67/MKI67 Antibody (1E1G1) Immunocytochemistry/ Immunofluorescence: Ki67/MKI67 Antibody (1E1G1) [NBP3-57756]

Immunocytochemistry/ Immunofluorescence: Ki67/MKI67 Antibody (1E1G1) [NBP3-57756]

Confocal imaging of PC-12 cells using NBP3-57756 (dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). The cells were counterstained with alpha-Tubulin Mouse mAb (dilution 1:400) followed by incubation with conjugated Goat Anti-Mouse IgG (H+L) Ab (dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
Ki67/MKI67 Antibody (1E1G1) Immunohistochemistry-Paraffin: Ki67/MKI67 Antibody (1E1G1) [NBP3-57756]

Immunohistochemistry-Paraffin: Ki67/MKI67 Antibody (1E1G1) [NBP3-57756]

Confocal imaging of paraffin-embedded Mouse spleen tissue using NBP3-57756 (dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.
Ki67/MKI67 Antibody (1E1G1) Western Blot: Ki67/MKI67 Antibody (1E1G1) [NBP3-57756]

Western Blot: Ki67/MKI67 Antibody (1E1G1) [NBP3-57756]

Western blot analysis of lysates from wild type (WT) and Ki67 knockout (KO) HeLa cells using NBP3-57756 at 1:1000 dilution incubated overnight at 4C. HeLa cells and Ki67 knockout (KO) HeLa cells were treated by Nocodazole (1 ug/mL) at 37C for 24 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 30 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Exposure time: 10s.
Ki67/MKI67 Antibody (1E1G1) Western Blot: Ki67/MKI67 Antibody (1E1G1) [NBP3-57756]

Western Blot: Ki67/MKI67 Antibody (1E1G1) [NBP3-57756]

Western blot analysis of lysates from RAW264.7 cells using NBP3-57756 at 1:1000 dilution incubated overnight at 4C. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Exposure time: 5s.
Ki67/MKI67 Antibody (1E1G1) Immunohistochemistry-Frozen: Ki67/MKI67 Antibody (1E1G1) [NBP3-57756]

Immunohistochemistry-Frozen: Ki67/MKI67 Antibody (1E1G1) [NBP3-57756]

Confocal imaging of frozen sections of Mouse colon tissue using NBP3-57756 (dilution 1:200) followed by a further incubation with Cy3-conjugated Goat anti-Rabbit IgG (H+L) (dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Microwave antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.
Ki67/MKI67 Antibody (1E1G1) Immunohistochemistry-Frozen: Ki67/MKI67 Antibody (1E1G1) [NBP3-57756]

Immunohistochemistry-Frozen: Ki67/MKI67 Antibody (1E1G1) [NBP3-57756]

Confocal imaging of frozen sections of Mouse spleen tissue using NBP3-57756 (dilution 1:200) followed by a further incubation with Cy3-conjugated Goat anti-Rabbit IgG (H+L) (dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Microwave antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.
Ki67/MKI67 Antibody (1E1G1) Immunohistochemistry-Frozen: Ki67/MKI67 Antibody (1E1G1) [NBP3-57756]

Immunohistochemistry-Frozen: Ki67/MKI67 Antibody (1E1G1) [NBP3-57756]

Confocal imaging of frozen sections of Rat colon tissue using NBP3-57756 (dilution 1:200) followed by a further incubation with Cy3-conjugated Goat anti-Rabbit IgG (H+L) (dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Microwave antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.
Ki67/MKI67 Antibody (1E1G1) Immunohistochemistry-Frozen: Ki67/MKI67 Antibody (1E1G1) [NBP3-57756]

Immunohistochemistry-Frozen: Ki67/MKI67 Antibody (1E1G1) [NBP3-57756]

Confocal imaging of frozen sections of Rat spleen tissue using NBP3-57756 (dilution 1:200) followed by a further incubation with Cy3-conjugated Goat anti-Rabbit IgG (H+L) (dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Microwave antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.
Ki67/MKI67 Antibody (1E1G1) Simple Western: Ki67/MKI67 Antibody (1E1G1) [NBP3-57756]

Simple Western: Ki67/MKI67 Antibody (1E1G1) [NBP3-57756]

Simple Western analysis of lysates from HeLa cells using NBP3-57756 at 1:100 dilution. The virtual lane view (left) shows the target band (as indicated) with samples in concentrations of 0.2 mg/mL, 0.7 mg/mL and 2.0 mg/mL. The corresponding electropherogram view (right) plots chemiluminescence intensity against molecular weight along the capillary for sample concentrations of 0.2 mg/mL, 0.7 mg/mL and 2.0 mg/mL. This experiment was performed under reducing conditions on the Jess Simple Western instrument using the 66-440 kDa separation module.
Ki67/MKI67 Antibody (1E1G1) Immunohistochemistry-Paraffin: Ki67/MKI67 Antibody (1E1G1) [NBP3-57756]

Immunohistochemistry-Paraffin: Ki67/MKI67 Antibody (1E1G1) [NBP3-57756]

Immunohistochemistry analysis of paraffin-embedded Human esophagus tissue using NBP3-57756 at a dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Ki67/MKI67 Antibody (1E1G1) Immunohistochemistry-Paraffin: Ki67/MKI67 Antibody (1E1G1) [NBP3-57756]

Immunohistochemistry-Paraffin: Ki67/MKI67 Antibody (1E1G1) [NBP3-57756]

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using NBP3-57756 at a dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Ki67/MKI67 Antibody (1E1G1) Immunohistochemistry-Paraffin: Ki67/MKI67 Antibody (1E1G1) [NBP3-57756]

Immunohistochemistry-Paraffin: Ki67/MKI67 Antibody (1E1G1) [NBP3-57756]

Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using NBP3-57756 at a dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Ki67/MKI67 Antibody (1E1G1) Immunohistochemistry-Paraffin: Ki67/MKI67 Antibody (1E1G1) [NBP3-57756]

Immunohistochemistry-Paraffin: Ki67/MKI67 Antibody (1E1G1) [NBP3-57756]

Immunohistochemistry analysis of paraffin-embedded Mouse colon tissue using NBP3-57756 at a dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Ki67/MKI67 Antibody (1E1G1) Immunohistochemistry-Paraffin: Ki67/MKI67 Antibody (1E1G1) [NBP3-57756]

Immunohistochemistry-Paraffin: Ki67/MKI67 Antibody (1E1G1) [NBP3-57756]

Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using NBP3-57756 at a dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Ki67/MKI67 Antibody (1E1G1) Immunohistochemistry-Paraffin: Ki67/MKI67 Antibody (1E1G1) [NBP3-57756]

Immunohistochemistry-Paraffin: Ki67/MKI67 Antibody (1E1G1) [NBP3-57756]

Immunohistochemistry analysis of paraffin-embedded Rat spleen tissue using NBP3-57756 at a dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

Applications for Ki67/MKI67 Antibody (1E1G1)

Application
Recommended Usage

ELISA

1 ug/mL

Immunocytochemistry/ Immunofluorescence

1:200 - 1:800

Immunohistochemistry-Paraffin

1:1000 - 1:8000

Western Blot

1:1000 - 1:6000
Application Notes
For immunofluorescence on paraffin-embedded tissues, the recommended concentration is 1:200 - 1:800

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, (pH 7.3)

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Ki67/MKI67

Ki67 is a nuclear protein associated with proliferation, named after its discovery in a Hodgkin's lymphoma-derived cell line by Scholzer and Gerdes at Kiel University, Germany (1). This proliferation marker is expressed during active phases of the cell cycle (late G1, S, G2 and M), whereas the protein remains undetectable in the G0 phase. Ki67 has two main isoforms with theoretical molecular weights of 319 kDa and 359 kDa. Its abundance is tightly regulated by synthesis and degradation with an estimated half-life of 60-90 min, regardless of its position in the cell cycle. Ki67 has been shown to be involved in ribosomal biogenesis, heterochromatin maintenance, and mitotic chromosome separation (2).

Detection of Ki67 by immunostaining is commonly used as a proliferation marker in solid tumors, as well as certain hematological malignancies (3-5). The Ki67 index, which reports on positive Ki67 stained tumor cell nuclei, has been extensively studied as a prognostic biomarker in cancers such as breast cancer and cervical cancer.

References

1. Gerdes J, Schwab U, Lemke H, Stein H. (1983) Production of a mouse monoclonal antibody reactive with a human nuclear antigen associated with cell proliferation. Int J Cancer. 31:13-20. PMID: 6339421

2. Starborg M, Gell K, Brundell E and Hoog C. (1996) The murine Ki-67 cell proliferation antigen accumulates in the nucleolar and heterochromatic regions of interphase cells and at the periphery of the mitotic chromosomes in a process essential for cell cycle progression. J Cell Sci. 109:143-153. 1996

3. Karamitopoulou E, Perentes E, Tolnay M, Probst A. (1998) Prognostic significance of MIB-1, p53, and bcl-2 immunoreactivity in meningiomas. Hum Pathol. 29(2):140-5. PMID: 9490273

4. Geyer FC, Rodrigues DN, Weigelt B and Reis-Filho JS. (2012) Molecular classification of estrogen receptor-positive/luminal breast cancers. Adv Anat Pathol. 19(1):39-53. PMID: 22156833

5. Ikenberg H, Bergeron C, Schmidt D, Griesser H, Alameda F, Angeloni C, Bogers J, Dachez R, Denton K, Hariri J, Keller T, von Knebel Doeberitz M, Neumann HH, Puig-Tintore LM, Sideri M, Rehm S, Ridder R; PALMS Study Group. (2013) Screening for cervical cancer precursors with p16/Ki-67 dual-stained cytology: results of the PALMS study. J Natl Cancer Inst. 105(20):1550-7. PMID: 24096620

Long Name

Antigen Identified by Monoclonal Antibody Ki67

Alternate Names

Ki-67, KIA, MIB-1, MKI67, PPP1R105, TSG126

Gene Symbol

MKI67

Additional Ki67/MKI67 Products

Product Documents for Ki67/MKI67 Antibody (1E1G1)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Ki67/MKI67 Antibody (1E1G1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cancer Biomarkers
Cell Cycle

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Ki67/MKI67 Antibody (1E1G1)

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  • Q: Whats the concentration of this Ki67/MKI67 Antibody?

    A: The concentration is lot specific available upon request.

  • Q: Whats the concentration of this Ki67/MKI67 Antibody?

    A:  Optimal concentrations and conditions for each application should be determined by the user.

  • Q: Whats the concentration of this Ki67/MKI67 Antibody?

    A: The concentration is lot specific available upon request.

  • Q: Whats the concentration of this Ki67/MKI67 Antibody?

    A:  Optimal concentrations and conditions for each application should be determined by the user.

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