Kinesin 5A Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-76816

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Kinesin 5A Primary Antibodies »

Product Specifications

Immunogen

Antibody was raised against a 20 amino acid synthetic peptide from near the carboxy terminus of human KIF5. The immunogen is located within amino acids 880 - 930 of KIF5.

Specificity

At least three isoforms of KIF5 are known to exist; this antibody will recognize all three isoforms.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

116 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Kinesin 5A Antibody - BSA Free

Western Blot: Kinesin 5A AntibodyBSA Free [NBP1-76816]

Western Blot: Kinesin 5A AntibodyBSA Free [NBP1-76816]

Western Blot: Kinesin 5A Antibody [NBP1-76816] - K562 cell lysate with Kinesin 5A antibody at (A) 0.5 and (B) 1 ug/ml.
Western Blot: Kinesin 5A AntibodyBSA Free [NBP1-76816]

Western Blot: Kinesin 5A AntibodyBSA Free [NBP1-76816]

Western Blot: Kinesin 5A Antibody [NBP1-76816] - Human samples tested and cleaned up bands after speaking with technical service and changing wash protocol. Image submitted by a verified customer review.
Immunocytochemistry/ Immunofluorescence: Kinesin 5A Antibody - BSA Free [NBP1-76816]

Immunocytochemistry/ Immunofluorescence: Kinesin 5A Antibody - BSA Free [NBP1-76816]

Immunocytochemistry/Immunofluorescence: Kinesin 5A Antibody [NBP1-76816] - K562 cells with Kinesin 5A antibody at 5 ug/ml.
Kinesin 5A Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Kinesin 5A Antibody - BSA Free [NBP1-76816] -

Immunocytochemistry/ Immunofluorescence: Kinesin 5A Antibody - BSA Free [NBP1-76816] - Immunofluorescence of Kinesin 5A in K562 cells with Kinesin 5A antibody at 20 ug/mL.
Kinesin 5A Antibody - BSA Free

Immunohistochemistry: Kinesin 5A Antibody - BSA Free [NBP1-76816] -

Immunohistochemistry: Kinesin 5A Antibody - BSA Free [NBP1-76816] - Immunohistochemistry of Kinesin 5A in mouse brain tissue with Kinesin 5A antibody at 5 ug/ml.
Kinesin 5A Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Kinesin 5A Antibody - BSA Free [NBP1-76816] -

Immunocytochemistry/ Immunofluorescence: Kinesin 5A Antibody - BSA Free [NBP1-76816] - Immunofluorescence of Kinesin 5A in mouse brain tissue with Kinesin 5A antibody at 20 ug/mL.

Red: Kinesin 5A Antibody
Blue: DAPI staining

Applications for Kinesin 5A Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

5-20 ug/mL

Immunohistochemistry

5 ug/ml

Western Blot

0.5-1 ug/ml
Application Notes
Kinesin 5A antibody validated for WB from a verified customer review.

Reviewed Applications

Read 1 review rated 4 using NBP1-76816 in the following applications:

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Kinesin 5A

The kinesin superfamily proteins (KIFs) are microtubule-dependent motors that slide along microtubules and transport cellular organelles and mRNA to different parts of the cell. In neurons, KIF5 performs this role in an anterograde fashion from the neuronal cell body to axonal or dendritic terminals. KIF5 was initially differentiated from the ubiquitously expressed kinesin as being highly enriched in subsets of neurons and selectively concentrated in the cell body. KIF5 has at least three known isoforms; this antibody recognizes all three. Mutations in one of the isoforms of KIF5 (KIF5A) are thought to be involved in hereditary spastic paraplegias.

Alternate Names

D12S1889, KIF5A variant protein, kinesin family member 5A, kinesin heavy chain isoform 5A, Kinesin heavy chain neuron-specific 1, kinesin, heavy chain, neuron-specific, MY050, Neuronal kinesin heavy chain, NKHC1, NKHCspastic paraplegia 10 (autosomal dominant), SPG10

Gene Symbol

KIF5A

UniProt

Q12840 (Human)

Additional Kinesin 5A Products

Product Documents for Kinesin 5A Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Kinesin 5A Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for Kinesin 5A Antibody - BSA Free (1)

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Customer Images

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  • Name: Anonymous
    Application: Western Blot
    Sample Tested:
    Species: Human
    Verified Customer | Posted 02/28/2014
    Picture of bands with 1/1500 anti-kif5a antibody, secondary 1:2000, 10 minutes exposure
    Kinesin 5A Antibody - BSA Free NBP1-76816

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Protocols

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FAQs

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