Kir6.1 Antibody (S366-60) - BSA Free

Novus Biologicals | Catalog # NBP2-59324

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Gerbil

Cited:

Rat, Gerbil

Applications

Validated:

Immunohistochemistry, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # S366-60

Format

BSA Free
View all Kir6.1 Primary Antibodies »

Product Specifications

Immunogen

Fusion protein amino acids 306-424 (Cytoplasmic C-terminus) of rat Kir6.1

Reactivity Notes

Use in Gerbil reported in scientific literature (PMID:31573045).

Localization

Membrane

Specificity

Detects 45kDa.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Kir6.1 Antibody (S366-60) - BSA Free

Western Blot: Kir6.1 Antibody (S366-60) [NBP2-59324]

Western Blot: Kir6.1 Antibody (S366-60) [NBP2-59324]

Western Blot: Kir6.1 Antibody (S366-60) [NBP2-59324] - Western Blot analysis of Rat Brain showing detection of ~45 kDa Kir6.1 protein using Mouse Anti-Kir6.1 Monoclonal Antibody, Clone S366-60 (NBP2-59324). Lane 1: MW Ladder. Lane 2: Rat Brain. Load: 20 ug. Block: 2% GE Healthcare Blocker for 1 hour at RT. Primary Antibody: Mouse Anti-Kir6.1 Monoclonal Antibody (NBP2-59324) at 1:1000 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~45 kDa. Other Band(s): ~100 kDa.
Immunocytochemistry/ Immunofluorescence: Kir6.1 Antibody (S366-60) [NBP2-59324]

Immunocytochemistry/ Immunofluorescence: Kir6.1 Antibody (S366-60) [NBP2-59324]

Immunocytochemistry/Immunofluorescence: Kir6.1 Antibody (S366-60) [NBP2-59324] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Kir6.1 Monoclonal Antibody, Clone S366-60 (NBP2-59324). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-Kir6.1 Monoclonal Antibody (NBP2-59324) at 1:100 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Kir6.1 Antibody (D) Composite.
Immunocytochemistry/ Immunofluorescence: Kir6.1 Antibody (S366-60) [NBP2-59324]

Immunocytochemistry/ Immunofluorescence: Kir6.1 Antibody (S366-60) [NBP2-59324]

Immunocytochemistry/Immunofluorescence: Kir6.1 Antibody (S366-60) [NBP2-59324] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Kir6.1 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Membrane. Magnification: 60X.

Applications for Kir6.1 Antibody (S366-60) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 50% Glycerol

Format

BSA Free

Preservative

0.1% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Background: Kir6.1

The Kir6.1 (KCNJ8) gene is essential in membrane function as it encodes an inward-rectifier potassium channel protein that is 424 amino acids long and has a mass of nearly 48 kDA. Dysfunctions in the Kir6.1 gene may be a cause of J-wave syndromes and sudden infant death syndrome (SIDS). Research is investigating its role in various diseases such as retinitis, hypoxia, pancreatitis, myocardial infarction, spasticity, leiomyoma, acute myocardial infarction, and prinzmetal angina. The Kir6.1 gene plays a significant role in ATP sensitive potassium channels, SIDS susceptibility pathways, dopamine feedback into cAMP pathway, and the CFTR-dependent regulation of ion channels in airway epithelium. It interacts with other genes such as KCNJ2, ABCC8, ABCC9, and KCNJ11.

Long Name

ATP-sensitive inward rectifier potassium channel 8

Alternate Names

KCNJ8, uKATP-1

Gene Symbol

KCNJ8

Additional Kir6.1 Products

Product Documents for Kir6.1 Antibody (S366-60) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Kir6.1 Antibody (S366-60) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Kir6.1 Antibody (S366-60) - BSA Free

Customer Reviews for Kir6.1 Antibody (S366-60) - BSA Free

There are currently no reviews for this product. Be the first to review Kir6.1 Antibody (S366-60) - BSA Free and earn rewards!

Have you used Kir6.1 Antibody (S366-60) - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies