KLF15 Antibody - BSA Free

Western Blot: KLF15 Antibody - BSA Free [NBP2-24635] -

BCAA catabolic enzyme expression and BCKDH enzyme activity is decreased in the SAT of cardiac surgery patients with severe obesity. (A) Free plasma BCAA levels measured by UPLC MSMS in the non-obese (n = 8), pre-obese (n = 6) and class I obese (n = 7), class II obese (n = 6) and class III obese (n = 6) cardiac surgery patients. (B) Quantification of ACADSB, BCAT2,HADHA, PPM1K, KLF15, BCKDHA, BCKDHB, and BCKDK mRNA expression corrected to beta -actin and SDHA (39) and expressed as fold change in the SAT of non-obese (n = 6), pre-obese (n = 6) and obese class I (n = 6), class II (n = 6) and class III (n = 6) cardiac surgery patients. (C) Representative immunoblot and densitometric analysis of KLF15, pBCKDE1a, BCKDH, BCKDK, and DLD protein levels in the SAT of non-obese (n = 3), pre-obese (n = 3), class I obese (n = 4) and class II obese (n = 3) cardiac surgery patients. (D) Total and actual BCKDH enzyme activity corrected to protein levels, measured at Vmax (t = 15 min) in the SAT of non-obese (n = 7), pre-obese (n = 9), class I obese (n = 9), class II obese (n = 9) and class III obese (n = 8). Statistical analysis was performed using a two-way ANOVA followed by a Tukey's multiple comparison test; *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 as indicated (40). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/32903728), licensed under a CC-BY license. Not internally tested by Novus Biologicals.