KLH Antibody (3B1) - BSA Free

Novus Biologicals | Catalog # NBP2-44049

Novus Biologicals
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Key Product Details

Species Reactivity

All Species

Applications

Immunohistochemistry, Western Blot, ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgM Clone # 3B1

Format

BSA Free
View all KLH Primary Antibodies »

Product Specifications

Immunogen

KLH Antibody (3B1) was produced in mouse by repeated immunizations with KLH (Keyhole Limpet Hemocyanin) followed by hybridoma development.

Reactivity Notes

KLH antibody is specific for KLH. Does not cross react with mouse heart or skin tissue sections. Cross-reactivity from other sources has not been determined.

Clonality

Monoclonal

Host

Mouse

Isotype

IgM

Description

This antibody has been prepared from mouse ascitic fluid by immunoaffinity chromatography

Store this antibody at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Applications for KLH Antibody (3B1) - BSA Free

Application
Recommended Usage

ELISA

1:20000-1:100000

Immunohistochemistry

1:1000-1:5000

Western Blot

1:2000-1:10000
Application Notes
This product can be used as an IgM isotype control in various assays or in ELISA assays to detect KLH or KLH conjugates. Specific conditions for reactivity should be optimized by the end user.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

0.1 M Tris Chloride, 0.5 M Sodium Chloride, pH 8.0

Format

BSA Free

Preservative

0.1% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: KLH

KLH (Keyhole Limpet Hemocyanin) is a copper containing oxygen carrier occurring freely dissolved in the hemolymph of many mollusks and arthropods. KLH is often used in molecular immunology as a carrier protein conjugated to low molecular weight molecules such as peptides, amino acids, nucleic acids, drugs or toxins to render them more immunogenic due to the size of the conjugate complex and the immunogenicity of KLH. KLH forms a large complex composed of ~50 kDa subunits.

Alternate Names

keyhole limpet hemocyanin (KLH)

Additional KLH Products

Product Documents for KLH Antibody (3B1) - BSA Free

Certificate of Analysis

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Product Specific Notices for KLH Antibody (3B1) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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