KMT1A/SUV39H1 Antibody

Novus Biologicals | Catalog # NBP1-21367

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Predicted:

Bovine (100%), Orangutan (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all KMT1A/SUV39H1 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide made to an N-terminal portion of the human KMT1A/SUV39H1 protein (between residues 1-100) [UniProt O43463]

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 28425504).

Localization

Nucleus.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for KMT1A/SUV39H1 Antibody

Western Blot: KMT1A/SUV39H1 Antibody [NBP1-21367]

Western Blot: KMT1A/SUV39H1 Antibody [NBP1-21367]

Western Blot: KMT1A/SUV39H1 Antibody [NBP1-21367] - Detection of human SUV39H1 by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-SUV39H1 antibody NBP1-21367 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 10 ug/mg lysate. SUV39H1 was also immunoprecipitated by a rabbit anti-SUV39H1 antibody recognizing a downstream epitope (B). Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).
Immunohistochemistry-Paraffin: KMT1A/SUV39H1 Antibody [NBP1-21367]

Immunohistochemistry-Paraffin: KMT1A/SUV39H1 Antibody [NBP1-21367]

Immunohistochemistry-Paraffin: KMT1A/SUV39H1 Antibody [NBP1-21367] - SUV39H1 expression in human glioma tissue grade IV. Image submitted by a verified customer review.
Western Blot: KMT1A/SUV39H1 Antibody [NBP1-21367]

Western Blot: KMT1A/SUV39H1 Antibody [NBP1-21367]

Western Blot: KMT1A/SUV39H1 Antibody [NBP1-21367] - Up:SUV39H1 expression in normal brain, human glioma tissue grades II, III and IV respectively. Down: Actin expression levels. Image submitted by a verified customer review.
Western Blot: KMT1A/SUV39H1 Antibody [NBP1-21367]

Western Blot: KMT1A/SUV39H1 Antibody [NBP1-21367]

Western Blot: KMT1A/SUV39H1 Antibody [NBP1-21367] - Up: SUV39H1 expression in C6 astrocytic cell line and human glioma cell lines GOS-3, T98G, U87MG and 1321N1. Down: Actin levels. Image submitted by a verified customer review.

Applications for KMT1A/SUV39H1 Antibody

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:1500

Immunoprecipitation

5- 10 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
IHC-P, WB reactivitiy reported in scientific literature (PMID: 23943221), and verified customer reviews. Use in ICC/IF reported in scientific literature (PMID:28425504).

Reviewed Applications

Read 4 reviews rated 3.8 using NBP1-21367 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: KMT1A/SUV39H1

SUV39H1 (suppressor of variegation 3-9 homolog 1) is a histone methyltransferase that contains a chromodomain and a C-terminal SET domain. It specifically trimethylates histone H3 at lys 9. Interaction of SUV39H1 with RB1 results in recruitment to histone H3. Trimethylation of H3 stimulates the binding of heterochromatin proteins ((CBX1, CBX3 and/or CBX5) which leads to epigenetic transcriptional repression in heterochromatin regions. Recent research has also shown that this protein interacts with oncogenes involved in AML. Treatment of AML cells with chaetocin caused induction of apotosis via direct and indirect modulation of SUV39H1 activity (PMID:21979880). This protein plays a critical role in heterochromatin organization, chromosome segregation, and mitotic progression.

Long Name

Histone-lysine N-methyltransferase SUV39H1

Alternate Names

EC 2.1.1.355, H3-K9-HMTase 1, KMT1A, SUV39H, SUV39H1

Entrez Gene IDs

6839 (Human)

Gene Symbol

SUV39H1

UniProt

O43463 (Human)

Additional KMT1A/SUV39H1 Products

Product Documents for KMT1A/SUV39H1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for KMT1A/SUV39H1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for KMT1A/SUV39H1 Antibody

Customer Reviews for KMT1A/SUV39H1 Antibody (4)

3.8 out of 5
4 Customer Ratings
5 Stars
0%
4 Stars
75%
3 Stars
25%
2 Stars
0%
1 Stars
0%

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Customer Images

Showing  1 - 4 of 4 reviews Showing All
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  • Name: Anonymous
    Application: Western Blot
    Sample Tested:
    Species: Human
    Verified Customer | Posted 01/29/2014
    Up: SUV39H1 expression in C6 astrocytic cell line and human glioma cell lines GOS-3, T98G, U87MG and 1321N1. Down: Actin levels
    KMT1A/SUV39H1 Antibody NBP1-21367
  • Name: Anonymous
    Application: Western Blot
    Sample Tested:
    Species: Human
    Verified Customer | Posted 01/29/2014
    Up:SUV39H1 expression in normal brain, human glioma tissue grades II, III and IV respectively. Down: Actin expression levels
    KMT1A/SUV39H1 Antibody NBP1-21367
  • Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested:
    Species: Human
    Verified Customer | Posted 01/29/2014
    SUV39H1 expression in human glioma tissue grade IV
    KMT1A/SUV39H1 Antibody NBP1-21367
  • Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: SUV39H1 expression in human glioma tissue grade II
    Species: Human
    Verified Customer | Posted 01/29/2014
    SUV39H1 expression in human glioma tissue grade II
    KMT1A/SUV39H1 Antibody NBP1-21367

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Showing  1 - 4 of 4 reviews Showing All

Protocols

View specific protocols for KMT1A/SUV39H1 Antibody (NBP1-21367):

Western Blot Protocol for KMT1A/SUV39H1 Antibody (NBP1-21367):
Western Blot Protocol

1. Perform SDS-PAGE on samples to be analyzed, loading 40 ug of total protein per lane.
2. Transfer proteins to membrane according to the instructions provided by the manufacturer of the membrane and transfer apparatus.
3. Stain according to standard Ponceau S procedure (or similar product) to assess transfer success, and mark molecular weight standards where appropriate.
4. Rinse the blot.
5. Block the membrane using standard blocking buffer for at least 1 hour.
6. Wash the membrane in wash buffer three times for 10 minutes each.
7. Dilute primary antibody in blocking buffer and incubate 1 hour at room temperature.
8. Wash the membrane in wash buffer three times for 10 minutes each.
9. Apply the diluted HRP conjugated secondary antibody in blocking buffer (as per manufacturers instructions) and incubate 1 hour at room temperature.
10. Wash the blot in wash buffer three times for 10 minutes each (this step can be repeated as required to reduce background).
11. Apply the detection reagent of choice in accordance with the manufacturers instructions.
Note: Tween-20 can be added to the blocking or antibody dilution buffer at a final concentration of 0.05-0.2%.


Immunohistochemistry-Paraffin Embedded Sections

Antigen Unmasking:
Bring slides to a boil in 10 mM sodium citrate buffer (pH 6.0) then maintain at a sub-boiling temperature for 10 minutes. Cool slides on bench-top for 30 minutes.

Staining:
1. Wash sections in deionized water three times for 5 minutes each.
2. Wash sections in wash buffer for 5 minutes.
3. Block each section with 100-400 ul blocking solution for 1 hour at room temperature.
4. Remove blocking solution and add 100-400 ul diluted primary antibody. Incubate overnight at 4 C.
5. Remove antibody solution and wash sections in wash buffer three times for 5 minutes each.
6. Add 100-400 ul biotinylated diluted secondary antibody. Incubate 30 minutes at room temperature.
7. Remove secondary antibody solution and wash sections three times with wash buffer for 5 minutes each.
8. Add 100-400 ul Streptavidin-HRP reagent to each section and incubate for 30 minutes at room temperature.
9. Wash sections three times in wash buffer for 5 minutes each.
10. Add 100-400 ul DAB substrate to each section and monitor staining closely.
11. As soon as the sections develop, immerse slides in deionized water.
12. Counterstain sections in hematoxylin.
13. Wash sections in deionized water two times for 5 minutes each.
14. Dehydrate sections.
15. Mount coverslips.


*The above information is only intended as a guide. The researcher should determine what protocol best meets their needs. Please follow safe laboratory procedures.

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FAQs

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