Ku70/XRCC6 Antibody (4C2-1A6) - Azide and BSA Free

Novus Biologicals | Catalog # H00002547-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Sandwich ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 4C2-1A6

Format

Azide and BSA Free
View all Ku70/XRCC6 Primary Antibodies »

Product Specifications

Immunogen

XRCC6 (AAH08343.1, 1 a.a. ~ 609 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MSGWESYYKTEGDEEAEEEQEENLEASGDYKYSGRDSLIFLVDASKAMFESQSEDELTPFDMSIQCIQSVYISKIISSDRDLLAVVFYGTEKDKNSVNFKNIYVLQELDNPGAKRILELDQFKGQQGQKRFQDMMGHGSDYSLSEVLWVCANLFSDVQFKMSHKRIMLFTNEDNPHGNDSAKASRARTKAGDLRDTGIFLDLMHLKKPGGFDISLFYRDIISIAEDEDLRVHFEESSKLEDLLRKVRAKETRKRALSRLKLKLNKDIVISVGIYNLVQKALKPPPIKLYRETNEPVKTKTRTFNTSTGGLLLPSDTKRSQIYGSRQIILEKEETEELKRFDDPGLMLMGFKPLVLLKKHHYLRPSLFVYPEESLVIGSSTLFSALLIKCLEKEVAALCRYTPRRNIPPYFVALVPQEEELDDQKIQVTPPGFQLVFLPFADDKRKMPFTEKIMATPEQVGKMKAIVEKLRFTYRSDSFENPVLQQHFRNLEALALDLMEPEQAVDLTLPKVEAMNKRLGSLVDEFKELVYPPDYNPEGKVTKRKHDNEGSGSKRPKVEYSEEELKTHISKGTLGKFTVPMLKEACRAYGLKSGLKKQELLEALTKHFQD

Specificity

XRCC6 - thyroid autoantigen 70kDa (Ku antigen)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Ku70/XRCC6 Antibody (4C2-1A6) - Azide and BSA Free

Western Blot: Ku70/XRCC6 Antibody (4C2-1A6) [H00002547-M01]

Western Blot: Ku70/XRCC6 Antibody (4C2-1A6) [H00002547-M01]

Western Blot: Ku70/XRCC6 Antibody (4C2-1A6) [H00002547-M01] - Analysis of XRCC6 expression in transfected 293T cell line by G22P1 monoclonal antibody (M01), clone 4C2-1A6.Lane 1: XRCC6 transfected lysate(70 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: Ku70/XRCC6 Antibody (4C2-1A6) [H00002547-M01]

Immunocytochemistry/ Immunofluorescence: Ku70/XRCC6 Antibody (4C2-1A6) [H00002547-M01]

Immunocytochemistry/Immunofluorescence: Ku70/XRCC6 Antibody (4C2-1A6) [H00002547-M01] - Analysis of monoclonal antibody to XRCC6 on HeLa cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: Ku70/XRCC6 Antibody (4C2-1A6) [H00002547-M01]

Immunohistochemistry-Paraffin: Ku70/XRCC6 Antibody (4C2-1A6) [H00002547-M01]

Immunohistochemistry-Paraffin: Ku70/XRCC6 Antibody (4C2-1A6) [H00002547-M01] - Analysis of monoclonal antibody to XRCC6 on formalin-fixed paraffin-embedded human breast cancer. Antibody concentration 3 ug/ml.
Western Blot: Ku70/XRCC6 Antibody (4C2-1A6) [H00002547-M01]

Western Blot: Ku70/XRCC6 Antibody (4C2-1A6) [H00002547-M01]

Western Blot: Ku70/XRCC6 Antibody (4C2-1A6) [H00002547-M01] - XRCC6 monoclonal antibody (M01), clone 4C2-1A6 Analysis of XRCC6 expression in C32.
Sandwich ELISA: Ku70/XRCC6 Antibody (4C2-1A6) [H00002547-M01] - Detection limit for recombinant GST tagged XRCC6 is approximately 0.1ng/ml as a capture antibody.

Applications for Ku70/XRCC6 Antibody (4C2-1A6) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against recominant protein, transfected lysate and cell lysate for WB. It has been used for IF, IHC-P and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Ku70/XRCC6

The p70/p80 autoantigen is a nuclear complex consisting of two subunits with molecular masses of approximately 70 and 80 kDa. The complex functions as a single-stranded DNA-dependent ATP-dependent helicase. The complex may be involved in the repair of nonhomologous DNA ends such as that required for double-strand break repair, transposition, and V(D)J recombination. High levels of autoantibodies to p70 and p80 have been found in some patients with systemic lupus erythematosus.

Long Name

Lupus Ku Autoantigen Protein 70

Alternate Names

CTC75, CTCBF, G22P1, ML8, TLAA, XRCC6

Entrez Gene IDs

2547 (Human)

Gene Symbol

XRCC6

OMIM

152690 (Human)

UniProt

AAH08343 (Human)

Additional Ku70/XRCC6 Products

Product Documents for Ku70/XRCC6 Antibody (4C2-1A6) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Ku70/XRCC6 Antibody (4C2-1A6) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Ku70/XRCC6 Antibody (4C2-1A6) - Azide and BSA Free

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Protocols

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FAQs

No product specific FAQs exist for this product.

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